Selective culturing and genus-specific PCR detection for identification of Aeromonas in tissue samples to assist the medico-legal diagnosis of death by drowning

Forensic Science International

Volumn 221, Issue 1-3, 2012, Pages 11-15

  Huys, Geert ^a   Coopman, Vera ^b   Van Varenbergh, Dirk ^c   Cordonnier, Jan ^b,d  

^a GHENT UNIVERSITY   (Belgium)

^b Eurofins Medinet Inc   (United States)

^c VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^d Chemiphar NV   (Belgium)

Author keywords

ADA medium; Aeromonas; Bone marrow; Drowning; PCR; Sternal puncture

Indexed keywords

BACTERIAL DNA;

AEROMONAS; AEROMONAS HYDROPHILA; AEROMONAS POPOFFII; AEROMONAS VERONII; ARTICLE; AUTOPSY; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BONE MARROW BIOPSY; BONE MARROW CULTURE; CAUSE OF DEATH; CONTROLLED STUDY; CULTURE MEDIUM; DNA EXTRACTION; DROWNING; GENE AMPLIFICATION; GENUS; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; LUNG PARENCHYMA; MEDICOLEGAL ASPECT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; VALIDITY;

EID: 84864373841     PISSN: 03790738     EISSN: 18726283     Source Type: Journal    
DOI: 10.1016/j.forsciint.2012.03.017     Document Type: Article

References (39)

1. Idris A., Berg R., Bierens J., Bossaert L., Branche C., Gabrielli A., Graves S., Handley A., Hoelle R., Morley P., Papa L., Pepe P., Quan L., Szpilman D., Wigginton J., Modell J. Recommended guidelines for uniform reporting of data from drowning. The " Utstein Style" . Circulation 2003, 108:2565-2574.
2. H. Roll, Leerboek der Gerechtelijke Geneeskunde voor de scholen tot opleiding van Ind. artsen, 's-Gravenhage, Martinus Nijhoff, 1918.
3. Lunetta P., Modell J. Macroscopical, Microscopical, and Laboratory Findings in Drowning Victims. A Comprehensive Review, Forensic Pathology Reviews, Vol. 3 2005, Humana Press Inc., Totowa, New Jersey, pp. 3-77.
4. Venneman B., Brinkmann B., Tod im Wasser D. Rechtsmedizin 2003, 13:201-215.
5. Modell J., Bellefleur M., Davis J. Drowning without aspiration: is this an appropriate diagnosis?. J. Forensic Sci. 1999, 44:1119-1123.
6. Usumoto Y., Sameshima N., Hikiji W., Tsuji A., Kudo K., Inoue H., Ikeda N. Electrolyte analysis of pleural effusion as an indicator of drowning in sea water and freshwater. J. Forensic Leg. Med. 2009, 16:321-324.
7. Timperman J. The detection of diatoms in the marrow of sternum as evidence of death by drowning. J. Forensic Med. 1962, 9:134-136.
8. Peabody A.J. Diatoms and drowning. A review. Med. Sci. Law 1980, 20:254-261.
9. Pollanen M.S., Cheung C., Chiasson D.A. The diagnostic value of the diatom test for drowning, I. Utility: a retrospective analysis of 771 cases of drowning in Ontario, Canada. J. Forensic Sci. 1997, 42(2):281-285.
10. Rohn E.J., Frade P.D. The role of diatoms in medicolegal investigation I: the history, contemporary science, and application of the Datiom test for drowning. Forensic Examin. 2006, 10-15.
11. Thakar M.K., Singh R. Diatomological mapping of water bodies for the diagnosis of drowning cases. J. Forensic Leg. Med. 2010, 17(1):18-25.
12. Saukko P., Knight B. Knight's Forensic Pathology 2004, Arnold, London.
13. Kakizaki E., Kozawan S., Tashiro N., Sakai M., Yukawa N. Detection of bacterioplankton in immersed cadavers using selective agar plates. Leg. Med. 2009, 11:S350-S353.
14. Suto M., Abe S., Nakamura H., Suzuki T., Itoh T., Kochi H., Hoshiai G., Hiraiwa K. Phytoplankton gene detection in drowned rabbits. Leg. Med. (Tokyo) 2003, 5:S142-S144.
15. Kakizaki E., Kozawa S., Imamura N., Uchiyama T., Nishida S., Sakai M., Yukawa N. Detection of marine and freshwater bacterioplankton in immersed victims: post-mortem bacterial invasion does not readily occur. J. Forensic Sci. 2011, 211:9-18.
16. Kakizaki E., Kozawa S., Sakai M., Yukawa N. Bioluminescent bacteria have potential as a marker of drowning in seawater: two immersed cadavers retrieved near estuaries. Leg. Med. 2009, 11(2):91-96.
17. Kakizaki E., Takahama K., Seo Y., Kozawa S., Sakai M., Yukawa N. Marine bacteria comprise a possible indicator of drowning in seawater. Forensic Sci. Int. 2008, 176(2-3):236-247.
18. Piette M., De Letter E. Drowning: still a difficult autopsy diagnosis. Forensic Sci. Int. 2006, 163(1-2):1-9.
19. Yen Yen L., Jayaprakash P.T. Prevalence of diatom frustules in non-vegetarian foodstuffs and its implications in interpreting identification of diatom frustules in drowning cases. Forensic Sci. Int. 2007, 170:1-7.
20. Aoyagi M., Iwadate K., Matsuyama N., Nara A., Shimada R., Ito Y. An applied diagnosis of drowning by detection of the aquatic bacterium with the PCR method: a case report. Acta Crim. Jpn. 2006, 72:49-53.
21. Suto M., Kato N., Abe S., Nakamura M., Tsuchiya R., Hiraiwa K. Detection of bacterial genes by PCR and its application to practical cases of death by drowning. Res. Pract. Forens. Med. 2007, 50:73-79. (in Japanese with English abstract).
22. Lucci A., Cirnelli A. A microbiological test for the diagnosis of death by drowning. Forensic Sci. Int. 2007, 168(1):34-36.
23. Lucci A., Campobasso C.P., Cirnelli A., Lorenzini A. A promising microbiological test for the diagnosis of drowning. Forensic Sci. Int. 2008, 182(1-3):20-26.
24. Suto M., Kato N., Abe S., Nakamura M., Tsuchiya R., Hiraiwa K. PRC detection of bacterial genes provides evidence of death by drowning. Leg. Med. 2009, 11:S354-S356.
25. Miskul'skii A.M. The use of bacteriological analysis of the blood in the diagnosis of death by drowning. Sud. Med. Ekspert. 1990, 33:26-28.
26. Aoyagi M., Iwadate K., Fukui K., Abe S., Sakai K., Maebashi K., Ochiai E., Nakamura M. A novel method for the diagnosis of drowning by detection of Aeromonas sobria with PCR method. Leg. Med. (Tokyo) 2009, 11(6):257-259.
27. Holmes P., Niccolls L.M., Sartory D.A. The ecology of mesophilic Aeromonas in the aquatic environment. The Genus Aeromonas 1996, 127-150. Wiley & Sons, Chichester, England. B. Austin, M. Altwegg, P.J. Gosling, S. Joseph (Eds.).
28. Hoetzenecker K., Ankersmit H.J., Lang G., Scheed A., Marta G., Jaksch P., Klepetko W. Considerations on infectious complications using a drowned lung for transplantation. Transpl. Int. 2010, 23:e32-e34.
29. Bossi-Küpher M., Genini A., Peduzzi R., Demarta A. Tracheobronchitis caused by Aeromonas veronii biovar sobria after near-drowning. Case report. J. Med. Microbiol. 2007, 56:1563-1564.
30. Ender P., Dolan M., Dolan D., Farmer C., Melcher G. Near-drowning-associated Aeromonas pneumonia. J. Emerg. Med. 1996, 14(6):737-741.
31. Ender P., Dolan M. Pneumonia associated with near-drowning. Clin. Infect. Dis. 1997, 25:896-907.
32. Miyake M., Iga K., Izumi C., Miyagawa A., Kobashi Y., Konishi T. Rapid Progressive Pneumonia Due to Aeromonas hydrophila shortly after near-drowning. Intern. Med. 2000, 39(12):1128-1130.
33. Vehmeyer S.B.W., Bloem R.M., Petit P.L.C. Microbiological screening of post-mortem bone donors-two case reports. J. Hosp. Infect. 2001, 47:193-197.
34. Janda J.M., Abbott S.L. The Genus Aeromonas: taxonomy, pathogenicity, and infection. Clin. Microbiol. Rev. 2010, 23:35-73.
35. Havelaar A.H., During M., Versteegh J.F.M. Ampicillin-dextrin agar for the enumeration of Aeromonas species in water by membrane filtration. J. Appl. Bacteriol. 1987, 62:279-287.
36. Pitcher D.G., Saunders N.A., Owen R.J. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol. 1989, 8:151-156.
37. Cascon A., Castillo J.A., Moral C.H., Salazar M.S., Marcos J.Y., Carrasco G.N. RFLP-PCR analysis of the araA gene as a taxonomic tool for the genus Aeromonas. FEMS Microbiol. Lett. 1997, 156:199-204.
38. Dickson G., Poulter R., Maas E., Probert, Kieser J. Marine bacterial succession as a potential indicator of postmortem submersion interval. Forensic Sci. Int. 2011, 209:1-10.
39. Tamaska L. On diatom demonstration in the bone marrow of drowned dead bodies. Deutsch Ges. Gerichtl. Med. 1961, 51:398-403.