Detection of marine and freshwater bacterioplankton in immersed victims: Post-mortem bacterial invasion does not readily occur

Forensic Science International

Volumn 211, Issue 1-3, 2011, Pages 9-18

  Kakizaki, Eiji ^a   Kozawa, Shuji ^a   Imamura, Nahoko ^a   Uchiyama, Taketo ^a,b   Nishida, Sho ^a   Sakai, Masahiro ^c   Yukawa, Nobuhiro ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

^b Miyazaki Pref Police Headquarters   (Japan)

^c UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

Brackish water; Diatom test; Drowning; Estuary; Marine bacteria

Indexed keywords

FRESH WATER;

ADULT; AGED; ARTICLE; AUTOLYSIS; AUTOPSY; BACTERIOPLANKTON; BACTERIUM DETECTION; BLOOD SAMPLING; CADAVER; CAUSE OF DEATH; CLINICAL ARTICLE; CONTROLLED STUDY; DIATOM; DROWNING; ESTUARY; FRESHWATER SPECIES; HUMAN; HUMAN TISSUE; IMMERSION; MARINE SPECIES; NONHUMAN; PRIORITY JOURNAL; SALINITY; SPECIES INVASION; VICTIM;

EID: 79960628996     PISSN: 03790738     EISSN: 18726283     Source Type: Journal    
DOI: 10.1016/j.forsciint.2011.03.036     Document Type: Article

References (34)

1. Saukko P., Knight B. Knight's Forensic Pathology: Immersion Deaths 2004, Arnold, London, pp. 406-408. 3rd ed.
2. Piette M.H.A., De Letter E.A. Drowning: still a difficult autopsy diagnosis. Forensic Sci. Int. 2006, 163:1-9.
3. Kakizaki E., Takahama K., Seo Y., Kozawa S., Sakai M., Yukawa N. Marine bacteria comprise a possible indicator of drowning in seawater. Forensic Sci. Int. 2008, 176:236-247.
4. Kakizaki E., Kozawa S., Sakai M., Yukawa N. Bioluminescent bacteria have potential as a marker of drowning in seawater: two immersed cadavers retrieved near estuaries. Legal Med. 2009, 11:91-96.
5. Kakizaki E., Kozawa S., Tashiro N., Sakai M., Yukawa N. Detection of bacterioplankton in immersed cadavers using selective agar plates. Legal Med. 2009, 11:S350-S353.
6. Mishulsky A.M. Use of bacteriologic analysis of blood in diagnosis of death from drowning. Sud. Med. Ekspert. 1990, 33:26-28. (in Russian with English abstract).
7. Lucci A., Cirnelli A. A microbiological test for the diagnosis of death by drowning. Forensic Sci. Int. 2007, 168:34-36.
8. Zhu B.L., Ishikawa T., Michiue T., Quan L., Maeda H. Postmortem serum endotoxin level in relation to the causes of death. Legal Med. 2005, 7:103-109.
9. Aoyagi M., Iwadate K., Fukui K., Abe S., Sakai K., Maebashi K., Ochiai E., Nakamura M. A novel method for the diagnosis of drowning by detection of Aeromonas sobria with PCR method. Legal Med. 2009, 11:257-259.
10. Suto M., Kato N., Abe S., Nakamura M., Tsuchiya R., Hiraiwa K. Detection of bacterial genes by PCR and its application to practical cases of death by drowning. Res. Pract. Forensic Med. 2007, 50:73-79. (in Japanese with English abstract).
11. Lucci A., Campobasso C.P., Cirnelli A., Lorenzini G. A promising microbiological test for the diagnosis of drowning. Forensic Sci. Int. 2008, 182:20-26.
12. Munn C.B. Marine Microbiology: Ecology and Applications 2004, Bios Scientific Publishers, London, pp. 99-100.
13. Austin B. Marine Microbiology 1988, Cambridge University Press, Cambridge, pp. 32-42.
14. Sonnenwirth A.C. Gram-negative bacilli, vibrios, and spirilla. Gradwohl's Clinical Laboratory Methods and Diagnosis 1980, vol. 2:1731-1852. The C.V. Mosby Company, St. Louis. Eighth edition.
15. Lane D.J. 16S/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematics 1991, 115-175. John Wiley & Sons, New York. First edition. E. Stackebrandt, M. Goodfellow (Eds.).
16. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 1991, 173:697-703.
17. Suzuki M.T., Rappé M.S., Haimberger Z.W., Winfield H., Adair N., Ströbel J., Giovannoni S.J. Bacterial diversity among small-subunit rRNA gene clones and cellular isolates from the same seawater sample. Appl. Environ. Microbiol. 1997, 63:983-989.
18. Hiraishi A. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett. Appl. Microbiol. 1992, 15:210-213.
19. Hiraishi A., Shin Y.K., Ueda Y., Sugiyama J. Automated sequencing of PCR-amplified 16S rDNA on "Hydrolink" gels. J. Microbiol. Methods 1994, 19:145-154.
20. Richards G.P., Watson M.A., Crane E.J., Burt I.G., Bushek D. Shewanella and Photobacterium spp. in oysters and seawater from the Delaware Bay. Appl. Environ. Microbiol. 2008, 74:3323-3327.
21. Kakizaki E., Kozawa S., Matsuda H., Muraoka E., Uchiyama T., Sakai M., Yukawa N. In vitro study of possible microbial indicators for drowning: salinity and types of bacterioplankton proliferating in blood. Forensic Sci. Int. 2011, 204:80-87.
22. E. Kakizaki, S. Kozawa, M. Sakai, N. Yukawa, Numbers, sizes and types of diatoms around estuaries for a diatom test, Am. J. Forensic Med. Pathol., in press.
23. Kakizaki E., Kozawa S., Matsuda H., Muraoka E., Uchiyama T., Sakai M., Yukawa N. Freshwater bacterioplankton cultured from liver, kidney and lungs of a decomposed cadaver retrieved from a sandy seashore: possibility of drowning in a river and then floating out to sea. Legal Med. 2010, 12:195-199.
24. Hürlimann J., Feer P., Elber F., Niederberger K., Dirnhofer R., Wyler D. Diatom detection in the diagnosis of death by drowning. Int. J. Legal Med. 2000, 114:6-14.
25. Takeichi T., Kitamura O. Detection of diatom in formalin-fixed tissue by proteinase K digestion. Forensic Sci. Int. 2009, 190:19-23.
26. de la Grandmaison G.L., Leterreux M., Lasseuguette K., Alvarez J.-C., de Mazancourt P., Durigon M. Study of the diagnostic value of iron in fresh water drowning. Forensic Sci. Int. 2006, 157:117-120.
27. Inoue H., Ishida T., Tsuji A., Kudo K., Ikeda N. Electrolyte analysis in pleural effusion as an indicator of the drowning medium. Legal Med. 2005, 7:96-102.
28. Usumoto Y., Sameshima N., Hikiji W., Tsuji A., Kudo K., Inoue H., Ikeda N. Electrolyte analysis of pleural effusion as an indicator of drowning in seawater and freshwater. J. Forensic Legal Med. 2009, 16:321-324.
29. Maeda H., Zhu B.-L., Ishikawa T., Quan L., Michiue T., Bessho Y., Okazaki S., Kamikodai Y., Tsuda K., Komatsu A., Azuma Y. Analysis of postmortem biochemical findings with regard to the lung weight in drowning. Legal Med. 2009, 11:S269-S272.
30. Nishitani Y., Fujii K., Okazaki S., Imabayashi K., Matsumoto H. Weight ratio of the lungs and pleural effusion to the spleen in the diagnosis of drowning. Legal Med. 2006, 8:22-27.
31. Sugimura T., Kashiwagi M., Matsusue A., Hara K., Kageura M., Kubo S. Application of the drowning index to actual drowning cases. Legal Med. 2010, 12:68-72.
32. Hayashi T., Ishida Y., Mizunuma S., Kimura A., Kondo T. Differential diagnosis between freshwater drowning and saltwater drowning based on intrapulmonary aquaporin-5 expression. Int. J. Legal Med. 2009, 123:7-13.
33. An J.L., Ishida Y., Kimura A., Kondo T. Forensic application of intrarenal aquaporin-2 expression for differential diagnosis between freshwater and saltwater drowning. Int. J. Legal Med. 2010, 124:99-104.
34. Takahashi M., Kinoshita H., Nishiguchi M., Nishio H. Bromide detection in blood using energy dispersive X-ray fluorescence; a chemical marker supportive of drowning in seawater. Legal Med. 2010, 12:132-136.