Analytical variables influencing the HCV RNA determination by TaqMan real-time PCR in routine clinical laboratory practice

Molecular Biology Reports

Volumn 39, Issue 7, 2012, Pages 7421-7427

  Raza, Abida ^a   Ali, Zameer ^b   Irfan, Javaid ^a   Murtaza, Shahnaz ^a   Shakeel, Samina ^b  

^a PAKISTAN ATOMIC ENERGY COMMISSION   (Pakistan)

^b QUAID I AZAM UNIVERSITY   (Pakistan)

Author keywords

Analytical variables; Blood collection tubes; HCV; Pakistan; Real time PCR; Viral load

Indexed keywords

ANTICOAGULANT AGENT; VIRUS RNA;

ADULT; AEROSOL; ANALYTICAL PARAMETERS; ARTICLE; BLOOD SAMPLING; BLOOD STORAGE; CENTRIFUGATION; CLINICAL ARTICLE; CLINICAL LABORATORY; FREEZING; HEPATITIS C VIRUS; HUMAN; HUMAN TISSUE; LABORATORY DIAGNOSIS; LIQUID; MALE; NEEDLE; PLASMA; REAL TIME POLYMERASE CHAIN REACTION; TEMPERATURE; TREATMENT RESPONSE; VIRUS LOAD; VIRUS TITRATION;

ADULT; CLINICAL LABORATORY TECHNIQUES; HEPACIVIRUS; HEPATITIS C; HUMANS; MALE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; RNA, VIRAL; SPECIMEN HANDLING; VIRAL LOAD;

HEPATITIS C VIRUS;

EID: 84864283073     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-012-1574-3     Document Type: Article

References (32)

1. Malta FM, Medeiros-Filho JE, Azevedo RS, Goncalves L, Silva LC, Carrilho FJ et al (2010) Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis. Mem Inst Oswaldo Cruz 105(1):92-98
2. Blackham S, Baillie A, Al-Hababi F, Remlinger K, You S, Ha-matake R et al (2010) Gene expression profiling indicates the roles of host oxidative stress, apoptosis, lipid metabolism, and intracellular transport genes in the replication of hepatitis C virus. J Virol 84(10):5404-5414
3. Ferenci P (2008) Peginterferon and ribavirin in HCV: improvement of sustained viral response. Best Pract Res Clin Gastroen-terol 22(6):1109-1122
4. Aziz H, Gil ML, Waheed Y, Adeeb U, Raza A, Bilal I et al (2011) Evaluation of prognostic factors for peg interferon alfa-2b plus ribavirin treatment on HCV infected patients in Pakistan. Infect Genet Evol 11(3):640-645
5. Daniel HD, Grant PR, Garson JA, Tedder RS, Chandy GM, Abraham P (2008) Quantitation of hepatitis C virus using an in-house real-time reverse transcriptase polymerase chain reaction in plasma samples. Diagn Microbiol Infect Dis 61(4):415-420 (Pubitemid 351970649)
6. Pawlotsky JM, McHutchison JG (2004) Hepatitis C. Development of new drugs and clinical trials: promises and pitfalls. Summary of an AASLD hepatitis single topic conference, Chicago, IL, February 27-March 1 2003. Hepatology 39(2):554-567
7. Sener K, Yapar M, Bedir O, Gul C, Coskun O, Kubar A (2010) Stability of hepatitis C virus RNA in blood samples by TaqMan real-time PCR. J Clin Lab Anal 24(3):134-138
8. Chevaliez S, Bouvier-Alias M, Pawlotsky JM (2009) Performance of the Abbott real-time PCR assay using m2000sp and m2000rt for hepatitis C virus RNA quantification. J Clin Microbiol 47(6):1726-1732
9. Saldanha J, Heath A, Lelie N, Pisani G, Nubling M, Yu M (2000) Calibration of HCV working reagents for NAT assays against the HCV international standard. The collaborative study group. Vox Sang 78(4):217-224 (Pubitemid 30481252)
10. Laperche S, Bouchardeau F, Thibault V, Pozzetto B, Vallet S, Rosenberg AR et al (2007) Multicenter trials need to use the same assay for hepatitis C virus viral load determination. J Clin Microbiol 45(11):3788-3790 (Pubitemid 350156886)
11. Jose M, Curtu S, Gajardo R, Jorquera JI (2003) The effect of storage at different temperatures on the stability of hepatitis C virus RNA in plasma samples. Biologicals 31(1):1-8 (Pubitemid 36298658)
12. Wang JT, Wang TH, Sheu JC, Lin SM, Lin JT, Chen DS (1992) Effects of anticoagulants and storage of blood samples on efficacy of the polymerase chain reaction assay for hepatitis C virus. J Clin Microbiol 30(3):750-753
13. Kessler HH, Stelzl E, Raggam RB, Haas J, Kirchmeir F, Hegenbarth K et al (2001) Effects of storage and type of blood collection tubes on hepatitis C virus level in whole blood samples. J Clin Microbiol 39(5):1788-1790 (Pubitemid 32417021)
14. Pawlotsky JM (1997) Measuring hepatitis C viremia in clinical samples: can we trust the assays? Hepatology 26(1):1-4
15. Cuypers HT, Bresters D, Winkel IN, Reesink HW, Weiner AJ, Houghton M et al (1992) Storage conditions of blood samples and primer selection affect the yield of cDNA polymerase chain reaction products of hepatitis C virus. J Clin Microbiol 30(12):3220-3224
16. Quan CM, Krajden M, Zhao J, Chan AW (1993) High-performance liquid chromatography to assess the effect of serum storage conditions on the detection of hepatitis C virus by the polymerase chain reaction. J Virol Methods 43(3):299-307
17. Davis GL, Lau JY, Urdea MS, Neuwald PD, Wilber JC, Lindsay K et al (1994) Quantitative detection of hepatitis C virus RNA with a solid-phase signal amplification method: definition of optimal conditions for specimen collection and clinical application in interferon-treated patients. Hepatology 19(6):1337-1341
18. Grant PR, Kitchen A, Barbara JA, Hewitt P, Sims CM, Garson JA et al (2000) Effects of handling and storage of blood on the stability of hepatitis C virus RNA: implications for NAT testing in transfusion practice. Vox Sang 78(3):137-142 (Pubitemid 30248951)
19. Al Olaby RR, Azzazy HM (2011) Hepatitis C virus RNA assays: current and emerging technologies and their clinical applications. Expert Rev Mol Diagn 11(1):53-64
20. Berg T, Sarrazin C, Herrmann E, Hinrichsen H, Gerlach T, Zachoval R et al (2003) Prediction of treatment outcome in patients with chronic hepatitis C: significance of baseline parameters and viral dynamics during therapy. Hepatology 37(3):600-609 (Pubitemid 36258782)
21. Martinot-Peignoux M, Maylin S, Moucari R, Ripault MP, Boyer N, Cardoso AC et al (2009) Virological response at 4 weeks to predict outcome of hepatitis C treatment with pegylated interferon and ribavirin. Antiviral Ther 14(4):501-511
22. Cooper CL, MacPherson P, Cameron W (2006) Analytical and biological variables influencing quantitative hepatitis C virus (HCV) measurement in HIV-HCV coinfection. Can J Gastroenterol 20(1):31-35
23. Wang L, Zheng H, Deng W (1999) Quality control of plasma(-serum) specimen for the detection of HCV RNA with PCR. Zhonghua Gan Zang Bing Za Zhi 7(4):221-223
24. Miskovsky EP, Carrella AV, Gutekunst K, Sun CA, Quinn TC, Thomas DL (1996) Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus. J Clin Microbiol 34(8):1975-1979 (Pubitemid 26239235)
25. Hawkins A, Davidson F, Simmonds P (1997) Comparison of plasma virus loads among individuals infected with hepatitis C virus (HCV) genotypes 1, 2, and 3 by quantiplex HCV RNA assay versions 1 and 2, Roche monitor assay, and an in-house limiting dilution method. J Clin Microbiol 35(1):187-192
26. Lunel F, Cresta P, Vitour D, Payan C, Dumont B, Frangeul L et al (1999) Comparative evaluation of hepatitis C virus RNA quantitation by branched DNA, NASBA, and monitor assays. Hepatology 29(2):528-535 (Pubitemid 29088458)
27. Holodniy M, Mole L, Yen-Lieberman B, Margolis D, Starkey C, Carroll R et al (1995) Comparative stabilities of quantitative human immunodeficiency virus RNA in plasma from samples collected in vacutainer CPT, vacutainer PPT, and standard vac-utainer tubes. J Clin Microbiol 33(6):1562-1566
28. Watson J, Graves S, Ferguson J, D'Este C, Batey R (2007) Hepatitis C virus RNA quantitation and degradation studies in whole blood samples in vitro. Gut 56(2):306-307
29. Fernandes H, Morosyuk S, Abravaya K, Ramanathan M, Rainen L (2010) Evaluation of effect of specimen-handling parameters for plasma preparation tubes on viral load measurements obtained by using the Abbott real time HIV-1 load assay. J Clin Microbiol 48(7):2464-2468
30. Al-Soud WA, Radstrom P (2001) Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 39(2):485-493 (Pubitemid 32150805)
31. Pelt-Verkuil EV, Belkum AV, Hays JP (2008) Different types and varieties of nucleic acid target molecules. In: Principles and technical aspects of PCR amplification. Springer, Dordrecht, The Netherlands, pp 27-61
32. Hollingsworth RC, Sillekens P, Peter VD, Neal KR, Irving WL (1996) Serum HCV RNA levels assessed by quantitative NASBA®: stability of viral load over time, and lack of correlation with liver disease. J Hepatol 25(3):301-306 (Pubitemid 26323467)