Selection, phenotyping and identification of acid and hydrogen peroxide producing bacteria from vaginal samples of Canadian and East African women

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Schellenberg, John J ^a   Dumonceaux, Tim J ^b   Hill, Janet E ^c   Kimani, Joshua ^d   Jaoko, Walter ^d   Wachihi, Charles ^d   Mungai, Jane Njeri ^d   Lane, Margo ^a   Fowke, Keith R ^a,d   Ball, T Blake ^a,d,e   Plummer, Francis A ^a,d,e  

^a UNIVERSITY OF MANITOBA   (Canada)

^b SASKATOON RESEARCH CENTRE   (Canada)

^c UNIVERSITY OF SASKATCHEWAN   (Canada)

^d UNIVERSITY OF NAIROBI   (Kenya)

^e Public Health Agency of Canada   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ACID; CHAPERONIN; HYDROGEN PEROXIDE; MANNOSE; RIBOSOME RNA;

ADOLESCENT; AMPLICON; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BIFIDOBACTERIUM; CANADA; CAPILLARY ELECTROPHORESIS; CULTURE MEDIUM; FEMALE; GARDNERELLA; GENE SEQUENCE; HUMAN; HUMAN TISSUE; KENYA; LACTOBACILLUS; NONHUMAN; PHENOTYPE; PHENOTYPIC VARIATION; PROSTITUTION; STREPTOCOCCUS; VAGINA SMEAR;

ACIDS; ADOLESCENT; BACTERIA; CANADA; FEMALE; HIV INFECTIONS; HUMANS; HYDROGEN PEROXIDE; KENYA; PHENOTYPE; PROBIOTICS; RISK; VAGINA;

BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; GARDNERELLA; LACTOBACILLUS; STREPTOCOCCUS;

EID: 84864186753     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0041217     Document Type: Article

References (37)

1. Anukam KC, Osazuwa EO, Ahonkhai I, Reid G, (2006) Lactobacillus vaginal microbiota of women attending a reproductive health care service in Benin city, Nigeria. Sex Transm Dis 33: 59-62.
2. Beigi RH, Wiesenfeld HC, Hillier SL, Straw T, Krohn MA, (2005) Factors associated with absence of H2O2-producing Lactobacillus among women with bacterial vaginosis. J Infect Dis 191: 924-929.
3. Roberton AM, Wiggins R, Horner PJ, Greenwood R, Crowley T, et al. (2005) A novel bacterial mucinase, glycosulfatase, is associated with bacterial vaginosis. J Clin Microbiol 43: 5504-5508.
4. Westrom L, Evaldson G, Holmes KK, (1984) Taxonomy of vaginosis: bacterial vaginosis- a definition. Scand J Urol Nephrol Suppl 86: 259-264.
5. Forsum U, Hallen A, Larsson PG, (2005) Bacterial vaginosis-a laboratory and clinical diagnostics enigma. Apmis 113: 153-161.
6. Forsum U, Holst E, Larsson PG, Vasquez A, Jakobsson T, et al. (2005) Bacterial vaginosis-a microbiological and immunological enigma. Apmis 113: 81-90.
7. Larsson PG, Bergstrom M, Forsum U, Jacobsson B, Strand A, et al. (2005) Bacterial vaginosis. Transmission, role in genital tract infection and pregnancy outcome: an enigma. Apmis 113: 233-245.
8. Hillier SL, (1998) The vaginal microbial ecosystem and resistance to HIV. AIDS Res Hum Retroviruses 14 (Suppl 1): S17-21.
9. Boskey ER, Cone RA, Whaley KJ, Moench TR, (2001) Origins of vaginal acidity: high D/L lactate ratio is consistent with bacteria being the primary source. Hum Reprod 16: 1809-1813.
10. Boskey ER, Telsch KM, Whaley KJ, Moench TR, Cone RA, (1999) Acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification. Infect Immun 67: 5170-5175.
11. Ronnqvist PD, Forsgren-Brusk UB, Grahn-Hakansson EE, (2006) Lactobacilli in the female genital tract in relation to other genital microbes and vaginal pH. Acta Obstet Gynecol Scand 85: 726-735.
12. Klebanoff SJ, Coombs RW, (1991) Viricidal effect of Lactobacillus acidophilus on human immunodeficiency virus type 1: possible role in heterosexual transmission. J Exp Med 174: 289-292.
13. Fontaine EA, Taylor-Robinson D, (1990) Comparison of quantitative and qualitative methods of detecting hydrogen peroxide produced by human vaginal strains of lactobacilli. J Appl Bacteriol 69: 326-331.
14. Tao L, Pavlova S, Anzinger J, Carlson S, Jacobs A, et al. (2005) Fighting HIV with its natural enemy: Mannose-specific capture of HIV by Lactobacillus. Beneficial Microbes Conference, April 17-21, 2005, Poster Abstract #5B, Available: http://wwwasmorg/ASM/files/ccLibraryFiles/Filename/000000001988/Beneficial%20Micrboes%20Program%20and%20Abstract%20Bookpdf, Accessed:2009 June 23.
15. Reid G, (2006) Extra intestinal effects of prebiotics and probiotics. In: Gibson GR, Rastall RA, editors. Prebiotics: Development and Applications London, UK John Wiley and Sons, Ltd pp. 201-211.
16. Buffa V, Stieh D, Mamhood N, Hu Q, Fletcher P, et al. (2009) Cyanovirin-N potently inhibits human immunodeficiency virus type 1 infection in cellular and cervical explant models. J Gen Virol 90: 234-243.
17. O'Keefe BR, Vojdani F, Buffa V, Shattock RJ, Montefiori DC, et al. (2009) Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. Proc Natl Acad Sci U S A 106: 6099-6104.
18. Douek DC, Roederer M, Koup RA, (2009) Emerging concepts in the immunopathogenesis of AIDS. Annu Rev Med 60: 471-484.
19. Bolton M, van der Straten A, Cohen CR, (2008) Probiotics: potential to prevent HIV and sexually transmitted infections in women. Sex Transm Dis 35: 214-225.
20. Rabe LK, Hillier SL, (2003) Optimization of media for detection of hydrogen peroxide production by Lactobacillus species. J Clin Microbiol 41: 3260-3264.
21. Josephy PD, Eling T, Mason RP, (1982) The horseradish peroxidase-catalyzed oxidation of 3,5,3′,5′-tetramethylbenzidine. Free radical and charge-transfer complex intermediates. J Biol Chem 257: 3669-3675.
22. Al-Mushrif S, Jones BM, (1998) A study of the prevalence of hydrogen peroxide generating Lactobacilli in bacterial vaginosis: the determination of H2O2 concentrations generated, in vitro, by isolated strains and the levels found in vaginal secretions of women with and without infection. J Obstet Gynaecol 18: 63-67.
23. Song YL, Kato N, Matsumiya Y, Liu CX, Kato H, et al. (1999) Identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from Japanese women and newborn infants. J Clin Microbiol 37: 3062-3064.
24. Schellenberg JJ, Links MG, Hill JE, Dumonceaux TJ, Kimani J, et al. (2011) Molecular definition of vaginal microbiota in East African commercial sex workers. Appl Environ Microbiol 77: 4066-4074.
25. Hill JE, Penny SL, Crowell KG, Goh SH, Hemmingsen SM, (2004) cpnDB: a chaperonin sequence database. Genome Res 14: 1669-1675.
26. Hummelen R, Fernandes AD, Macklaim JM, Dickson RJ, Changalucha J, et al. (2010) Deep Sequencing of the Vaginal Microbiota of Women with HIV. PLoS One 5.
27. Schellenberg J, Blake Ball T, Lane M, Cheang M, Plummer F, (2008) Flow cytometric quantification of bacteria in vaginal swab samples self-collected by adolescents attending a gynecology clinic. J Microbiol Methods 73: 216-226.
28. Fowke KR, Dong T, Rowland-Jones SL, Oyugi J, Rutherford WJ, et al. (1998) HIV type 1 resistance in Kenyan sex workers is not associated with altered cellular susceptibility to HIV type 1 infection or enhanced beta-chemokine production. AIDS Res Hum Retroviruses 14: 1521-1530.
29. Fowke KR, Nagelkerke NJ, Kimani J, Simonsen JN, Anzala AO, et al. (1996) Resistance to HIV-1 infection among persistently seronegative prostitutes in Nairobi, Kenya. Lancet 348: 1347-1351.
30. Schellenberg J, Links MG, Hill JE, Dumonceaux TJ, Peters GA, et al. (2009) Pyrosequencing of the chaperonin-60 universal target as a tool for determining microbial community composition. Appl Environ Microbiol 75: 2889-2898.
31. Kilic AO, Pavlova SI, Alpay S, Kilic SS, Tao L, (2001) Comparative study of vaginal Lactobacillus phages isolated from women in the United States and Turkey: prevalence, morphology, host range, and DNA homology. Clin Diagn Lab Immunol 8: 31-39.
32. Ison CA, Hay PE, (2002) Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics. Sex Transm Infect 78: 413-415.
33. Nugent RP, Krohn MA, Hillier SL, (1991) Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 29: 297-301.
34. Cardinale M, Brusetti L, Quatrini P, Borin S, Puglia AM, et al. (2004) Comparison of different primer sets for use in automated ribosomal intergenic spacer analysis of complex bacterial communities. Appl Environ Microbiol 70: 6147-6156.
35. Saldanha AJ, (2004) Java Treeview-extensible visualization of microarray data. Bioinformatics 20: 3246-3248.
36. Hill JE, Paccagnella A, Law K, Melito PL, Woodward DL, et al. (2006) Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database. J Med Microbiol 55: 393-399.
37. Pearson WR, Lipman DJ, (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85: 2444-2448.