Application of label-free proteomics for differential analysis of lung carcinoma cell line A549

Methods in Molecular Biology

Volumn 893, Issue , 2012, Pages 241-248

  Sitek, Barbara ^a   Waldera Lupa, Daniel M ^a,b   Poschmann, Gereon ^a,b   Meyer, Helmut E ^a   Stühler, Kai ^b  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Label free quantification; Lung fibrosis; Peptide profiles; Transforming growth factor beta 1

Indexed keywords

CELL EXTRACT; PROTEOME; TRANSFORMING GROWTH FACTOR BETA1; TRYPSIN;

ARTICLE; CHEMISTRY; COMPUTER PROGRAM; ELECTROSPRAY MASS SPECTROMETRY; GEL CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; LUNG TUMOR; METABOLISM; PEPTIDE MAPPING; PHYSIOLOGY; PROTEIN DEGRADATION; PROTEOMICS; STATISTICAL ANALYSIS; TANDEM MASS SPECTROMETRY; TUMOR CELL LINE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

CELL EXTRACTS; CELL LINE, TUMOR; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DATA INTERPRETATION, STATISTICAL; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; LUNG NEOPLASMS; PEPTIDE MAPPING; PROTEOLYSIS; PROTEOME; PROTEOMICS; SOFTWARE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TRANSFORMING GROWTH FACTOR BETA1; TRYPSIN;

EID: 84864141891     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-885-6_16     Document Type: Article

References (2)

1. Bondarenko PV, Chelius D, Shaler TA (2002) Identification and relative quantitation of protein mixtures by enzymatic digestion followed by capillary reversed-phase liquid chromatography-tandem mass spectrometry. Anal Chem 74:4741-4749
2. Chelius D, Bondarenko PV (2002) Quantitative pro filing of proteins in complex mixtures using liquid chromatography and mass spectrometry. J Proteome Res 1:317-323