Generation of human fab libraries for phage display

Methods in Molecular Biology

Volumn 901, Issue , 2012, Pages 53-79

  Rader, Christoph ^a,b  

^a NATIONAL CANCER INSTITUTE   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Antibody fragment; Antibody libraries; Fab; Human monoclonal antibodies; Immune antibody repertoires; Library; Phage display; Phagemid

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY;

ANTIBODY AFFINITY; ARTICLE; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION;

ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION;

EID: 84864118140     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-931-0_4     Document Type: Article

References (12)

1. Beerli RR, Rader C (2010) Mining human antibody repertoires. MAbs 2:365-378
2. Webster R (2001) Filamentous phage biology. In: Barbas CF III, Burton DR, Scott JK, Silverman GJ (eds) Phage display, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
3. Sidhu SS (2001) Engineering M13 for phage display. Biomol Eng 18:57-63
4. Barbas CF III, Kang AS, Lerner RA, Benkovic SJ (1991) Assembly of combinatorial antibody libraries on phage surfaces: The gene III site. Proc Natl Acad Sci USA 88: 7978-7982
5. Hofer T, Tangkeangsirisin W, Kennedy MG, Mage RG, Raiker SJ, Venkatesh K, Lee H, Giger RJ, Rader C (2007) Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector. J Immunol Methods 318: 75-87
6. Baskar S, Suschak JM, Samija I, Srinivasan R, Childs RW, Pavletic SZ, Bishop MR, Rader C (2009) A human monoclonal antibody drug and target discovery platform for B-cell chronic lymphocytic leukemia based on allogeneic hematopoietic stem cell transplantation and phage display. Blood 114: 4494-4502
7. Andris-Widhopf J, Rader C, Barbas CF III (2001) Generation of antibody libraries: Immunization, RNA preparation, and cDNA synthesis. In: Barbas CF III, Burton DR, Scott JK, Silverman GJ (eds) Phage display, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
8. Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C (2008) Generation, affinity maturation, and characterization of a human antihuman NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J Mol Biol 384:1143-1156
9. Brown T, Mackey K, Du T (2004) Analysis of RNA by northern and slot blot hybridization. Curr Protoc Mol Biol chapter 4:unit 4 9
10. Scott JK, Barbas CF III (2001) Phage-display vectors. In: Barbas CF III, Burton DR, Scott JK, Silverman GJ (eds) Phage display, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
11. Zhu Z, Dimitrov DS (2009) Construction of a large naive human phage-displayed Fab library through one-step cloning. Methods Mol Biol 525:129-142, xv
12. Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF III (2000) Methods for the generation of chicken monoclonal antibody fragments by phage display. J Immunol Methods 242:159-181