Detection of Bacillus anthracis from spores and cells by loop-mediated isothermal amplification without sample preparation

Journal of Microbiological Methods

Volumn 90, Issue 3, 2012, Pages 280-284

  Dugan, Lawrence ^a   Bearinger, Jane ^a   Hinckley, Aubree ^a   Strout, Cheryl ^a   Souza, Brian ^a  

^a LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

Author keywords

Anthrax; Colorimetric detection; LAMP; Loop mediated isothermal amplification; Nucleic acid purification; Sample preparation; Spores

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACILLUS ANTHRACIS; BACTERIAL CELL; BACTERIAL CHROMOSOME; BACTERIAL SPORE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM CULTURE; BACTERIUM DETECTION; COLORIMETRY; CONTROLLED STUDY; DNA DETERMINATION; DNA PURIFICATION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; PLASMID; PRIORITY JOURNAL;

BACILLUS ANTHRACIS; BACTERIAL PROTEINS; DNA, BACTERIAL; LIMIT OF DETECTION; MICROBIAL VIABILITY; MOLECULAR TYPING; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SPORES, BACTERIAL;

BACILLUS ANTHRACIS;

EID: 84864110038     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.05.022     Document Type: Article

References (23)

1. Brittan H.G. The use of hydroxynaphthol blue in the ultramicro-determination of alkaline earth and lanthanide elements: an improved method. Anal. Chim. Acta 1978, 96:165-170.
2. Dukes J.P., King D.P., Alexanderson S. Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus. Arch. Virol. 2006, 151:1093-1106.
3. Francois P., Tangomo M., Hibbs J., Bonetti E.-J., Boehme C.C., Notomi T., Perkins M.D., Schrenzel J. Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications. FEMS Immunol. Med. Microbiol. 2011, 62:41-48.
4. Goto M., Honda E., Ogura A., Nomoto A., Hanaki K.-I. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxynaphthol blue. Biotech 2009, 46:167-172.
5. Haines R.B. CXCIX. The formation of bacterial proteases, especially in synthetic media. Biochem. J. 1931, 25:1851-1859.
6. Hatano B., Maki T., Obara T., Fukumoto H., Hagisawa K., Matsushita Y., Okutani A., Bazartseren B., Inoue S., Sata T., Katano H. LAMP using a disposable pocket warmer for Anthrax detection, a highly mobile and reliable method for anti-bioterrorism. Jpn. J. Infect. Dis. 2010, 63:36-40.
7. Hill G.M. Chemical factors iin the germination of spore-bearing aerobes. The effect of yeast extract on the germination of Bacillus anthracis and its replacement by adenosine. Biochem. J. 1949, 45:353-362.
8. Inglesby T.M., Henderson D.A., Bartlett J.G., Ascher M.S., Eitzen E., Friedlander A.M., Jauer J., McDade J., et al. Anthrax as a biological weapon. Medical and public health management. J. Am. Med. Assoc. 1999, 281:1735-1745.
9. Jain N., Kumar J.S., Parida M.M., Merwyn S., Rai G.P., Agarwal G.S. Real-time loop-mediated isothermal amplification assay for rapid and sensitive detection of anthrax spores in spiked soil and talcum powder. World J. Microbiol. Biotechnol. 2011, 27:1407-1413.
10. Jernigan J.A., Stephens D.S., Ashford D.A., Omenaca C., Topiel M.S., Galbraith M., Tapper M., Fisk T.L., et al. Bioterrorism-related inhalational Anthrax: the first 10 cases reported in the United States. Emerg. Infect. Dis. 2001, 7:933-944.
11. Keim P., Smith K., Keys C., Takahashi H., Kurata T., Kaufman A. Molecular investigation of the Aum Shinrykio anthrax release in Kameido, Japan. J. Clin. Microbiol. 2001, 39:4566-4567.
12. Kuboki N.N., Inoue T., Sakurai F.Di, Cello D.J., Grab H., Suzuki C.Sugimoto, Igarashi I. Loop-mediated isothermal amplification for detection of African trypanosomes. J. Clin. Microbiol. 2003, 41:5517-5524.
13. Kurosaki Y., Sakuma T., Fukuma A., Kawamoto K., Kamo N., Makin S.-l., Yasuda J. A simple and sensitive method for detection of Bacillus anthracis by loop-mediated isothermal amplification. J. Appl. Microbiol. 2009, 107:1947-1956.
14. Mikesell P., Ivins B.E., Ristroph J.D., Dreier T.M. Evidence for plasmid-mediated toxin production in Bacillus anthracis. Infect. Immun. 1983, 39:371-376.
15. Nagamine K., Hase T., Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell. Probes 2002, 16:223-229.
16. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000, 28:E63.
17. Ohtsuka K., Yanagawa K., Takatori K., Hara-Kudo Y. Detection of Salmonella enterica in naturally contaminated liquid eggs by loop-mediated isothermal amplification, and characterization of Salmonella isolates. Appl. Environ. Microbiol. 2005, 71:6730-6735.
18. Parida M., Sannarangaiah S., Dash P.K., Rao P.V.L., Morito K. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev. Med. Virol. 2008, 18:407-421.
19. Pile J.C., Malone J.D., Eitzen E.M., Friedlander A.M. Anthrax as a potential biological warfare agent. Arch. Intern. Med. 1998, 158:429-434.
20. Qiao Y.-M., Guo Y.-C., Zhang X.-E., Zhou Y.-F., Zhang Z.-P., Wei H.-P., Yang R.-F., Wang D.-B. Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores. Biotech. Lett. 2007, 29:1939-1946.
21. Read T.D., Peterson S.N., Tourasse N., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., et al. The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 2003, 423:81-86.
22. Saile E., Koehler T.M. Bacillus anthracis multiplication, persistence, and genetic exchange in the rhizosphere of grass plants. Appl. Environ. Microbiol. 2006, 72:3168-3174.
23. Wastling S.L., Picozzi K., Kakembo A.S.L., Welburn S.C. LAMP for human African trypanosomiasis: A comparative study of detection formats. PLoS Negl. Trop. Dis. 2010, 4:e865.