Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans Strain CH34

Applied and Environmental Microbiology

Volumn 78, Issue 12, 2012, Pages 4516-4518

  Rosier, Caroline ^a   Leys, Natalie ^b   Henoumont, Céline ^c   Mergeay, Max ^b   Wattiez, Ruddy ^a  

^a UNIVERSITY OF MONS   (Belgium)

^b Expert Group for Molecular and Cellular Biology   (Belgium)

^c NMR and Molecular Imaging Laboratory   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

2-BUTANONE; ACETONE CARBOXYLASE; GENOME ANALYSIS; KEY ENZYMES;

BACTERIA; BIODEGRADATION; CARBOXYLATION;

ACETONE;

ACETONE; ACETONE CARBOXYLASE; ALKANONE; CARBOXYLYASE; METHYLETHYL KETONE;

ACETONE; BACTERIUM; BIODEGRADATION; ENZYME ACTIVITY; GENOME;

ARTICLE; CHEMISTRY; CUPRIAVIDUS; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MULTIMERIZATION; PROTEIN SUBUNIT; TEMPERATURE;

ACETONE; BUTANONES; CARBOXY-LYASES; CUPRIAVIDUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; PROTEIN MULTIMERIZATION; PROTEIN SUBUNITS; SUBSTRATE SPECIFICITY; TEMPERATURE;

CUPRIAVIDUS METALLIDURANS; HELICOBACTERACEAE;

EID: 84864077228     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07974-11     Document Type: Article

References (23)

1. Birks SJ, Kelly DJ. 1997. Assay and properties of acetone carboxylase, a novel enzyme involved in acetone-dependent growth and CO2 fixation in Rhodobacter capsulatus and other photosynthetic and denitrifying bacteria. Microbiology 143:755-766.
2. Bonnet-Smits EM, Robertson LA, Van Dijken JP, Senior E, Kuenen JG. 1988. Carbon dioxide fixation as the initial step in the metabolism of acetone by Thiosphaera pantotropha. J. Gen. Microbiol. 134:2281-2289.
3. Boyd JM, Ellsworth H, Ensign SA. 2004. Bacterial acetone carboxylase is a manganese-dependent metalloenzyme. J. Biol. Chem. 279:46644-46651.
4. Brahmachary P, et al. 2008. The human gastric pathogen Helicobacter pylori has a potential acetone carboxylase that enhances its ability to colonize mice. BMC Microbiol. 8:14.
5. Chowdhury BR, Das SK. 2005. Acetone-a toxic chemical. Indian Chem. Eng. B 47:277-279.
6. Clark DD, Ensign SA. 1999. Evidence for an inducible nucleotidedependent acetone carboxylase in Rhodococcus rhodochrous B276. J. Bacteriol. 181:2752-2758.
7. Dullius CH, Chen CY, Schink B. 2011. Nitrate-dependent degradation of acetone by Alicycliphilus and Paracoccus strains and comparison of acetone carboxylase enzymes. Appl. Environ. Microbiol. 77:6821-6825.
8. Inui M, et al. 2008. Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl. Microbiol. Biotechnol. 77:1305-1316.
9. Janssen PH, Schink B. 1995. Catabolic and anabolic enzyme activities and energetics of acetone metabolism of the sulfate-reducing bacterium Desulfococcus biacutus. J. Bacteriol. 177:277-282.
10. Jobst B, Schühle K, Linne U, Heider J. 2010. ATP-dependent carboxylation of acetophenone by a novel type of carboxylase. J. Bacteriol. 192: 1387-1394.
11. Kalapos MP. 2003. On the mammalian acetone metabolism: from chemistry to clinical implications. Biochim. Biophys. Acta 1621:122-139.
12. +-dependent secondary alcohol dehydrogenase in propane metabolism by Gordonia sp. strain TY-5. J. Bacteriol. 185: 7120-7128.
13. Leys N, et al. 2009. The response of Cupriavidus metallidurans CH34 to spaceflight in the international space station. Antonie Van Leeuwenhoek 96:227-245.
14. Mergeay M, et al. 1985. Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals. J. Bacteriol. 162:328-334.
15. Nocek B, Boyd J, Ensign SA, Peters JW. 2004. Crystallization and preliminary X-ray analysis of an acetone carboxylase from Xanthobacter autotrophicus strain Py2. Acta Crystallogr. D Biol. Crystallogr. 60:385-387.
16. Schühle K, Heider J. 2012. Acetone and butanone metabolism of the denitrifying bacterium "Aromatoleum aromaticum" demonstrates novel biochemical properties of an ATP-dependent aliphatic ketone carboxylase. J. Bacteriol. 194:131-141.
17. Singh HB, et al. 1994. Acetone in the atmosphere: distribution, sources, and sinks. J. Geophys. Res. 99:1805-1819.
18. Sluis MK, Ensign SA. 1997. Purification and characterization of acetone carboxylase from Xanthobacter strain Py2. Proc. Natl. Acad. Sci. U. S. A. 94:8456-8461.
19. Sluis MK, et al. 2002. Biochemical, molecular, and genetic analyses of the acetone carboxylases from Xanthobacter autotrophicus strain Py2 and Rhodobacter capsulatus strain B10. J. Bacteriol. 184:2969-2977.
20. Sluis MK, Small FJ, Allen JR, Ensign SA. 1996. Involvement of an ATP-dependent carboxylase in a CO2-dependent pathway of acetone metabolism by Xanthobacter strain Py2. J. Bacteriol. 178:4020-4026.
21. Syldatk C, May O, Altenbuchner J, Mattes R, Siemann M. 1999. Microbial hydantoinases-industrial enzymes from the origin of life? Appl. Microbiol. Biotechnol. 51:293-309.
22. Taylor DG, Trudgill PW, Cripps RE, Harris PR. 1980. The microbial metabolism of acetone. J. Gen. Microbiol. 118:159-170.
23. Zheng YN, et al. 2009. Problems with the microbial production of butanol. J. Ind. Microbiol. Biotechnol. 36:1127-1138.