Heterogeneity of disease and clones of community-onset methicillin-resistant Staphylococcus aureus in children attending a paediatric hospital in Belgium

Clinical Microbiology and Infection

Volumn 18, Issue 8, 2012, Pages 769-777

  Vergison, A ^a   Machado, A Nobre ^a   Deplano, A ^a   Doyen, M ^a   Brauner, J ^a   Nonhoff, C ^a   de Mendonca R ^a   Mascart, G ^b   Denis, O ^a  

^a ERASME HOSPITAL   (Belgium)

^b HOP UNIV DES ENFANTS REINE FABIOLA   (Belgium)

Author keywords

Children; CO MRSA; HA MRSA; MRSA; Paediatrics

Indexed keywords

AMOXICILLIN PLUS CLAVULANIC ACID; ANTIBIOTIC AGENT; CEPHALOSPORIN; CIPROFLOXACIN; CLINDAMYCIN; COTRIMOXAZOLE; ENTEROTOXIN; PANTON VALENTINE LEUKOCIDIN;

ACUTE OTITIS MEDIA; ANTIBIOTIC RESISTANCE; ANTIBIOTIC THERAPY; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATION; BELGIUM; CHILD; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CYSTIC FIBROSIS; DISK DIFFUSION; DRUG TREATMENT FAILURE; FEMALE; GENE CASSETTE; GENETIC ANALYSIS; GENOTYPE; HEALTHCARE ASSOCIATED INFECTION; HOSPITAL ADMISSION; HUMAN; INFANT; MALE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS INFECTION; MULTILOCUS SEQUENCE TYPING; NONHUMAN; PEDIATRIC HOSPITAL; PHENOTYPE; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SCHOOL CHILD;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 84864065923     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2011.03637.x     Document Type: Article

References (45)

1. Kaplan SL, Hulten KG, Gonzalez BE et al. Three-year surveillance of community-acquired Staphylococcus aureus infections in children. Clin Infect Dis 2005; 40: 1785-1791.
2. Niniou I, Vourli S, Lebessi E et al. Clinical and molecular epidemiology of community-acquired, methicillin-resistant Staphylococcus aureus infections in children in central Greece. Eur J Clin Microbiol Infect Dis 2008: 27: 831-837.
3. Witte W. Community-acquired methicillin-resistant Staphylococcus aureus: what do we need to know? Clin Microbiol Infect 2009; 15: 17-25.
4. Daskalaki M, Rojo P, Marin-Ferrer M, Barrios M, Otero JR, Chaves F. Panton-Valentine leukocidin-positive Staphylococcus aureus skin and soft tissue infections among children in an emergency department in Madrid, Spain. Clin Microbiol Infect 2010; 16: 74-77.
5. Larsen AR, Stegger M, Bocher S, Sorum M, Monnet DL, Skov RL. Emergence and characterization of community-associated methicillin-resistant Staphyloccocus aureus infections in Denmark, 1999 to 2006. J Clin Microbiol 2009; 47: 73-78.
6. Del Giudice P, Blanc V, Durupt F et al. Emergence of two populations of methicillin-resistant Staphylococcus aureus with distinct epidemiological, clinical and biological features, isolated from patients with community-acquired skin infections. Br J Dermatol 2006; 154: 118-124.
7. Otter JA, French GL. Molecular epidemiology of community-associated meticillin-resistant Staphylococcus aureus in Europe. Lancet Infect Dis 2010; 10: 227-239.
8. Stenhem M, Ortqvist A, Ringberg H et al. Imported methicillin-resistant Staphylococcus aureus, Sweden. Emerg Infect Dis 2010; 16: 189-196.
9. Zetola N, Francis JS, Nuermberger EL, Bishai WR. Community-acquired meticillin-resistant Staphylococcus aureus: an emerging threat. Lancet Infect Dis 2005; 5: 275-286.
10. Chambers HF, Deleo FR. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol 2009; 7: 629-641.
11. David MZ, Daum RS. Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clin Microbiol Rev 2010; 23: 616-687.
12. Fang H, Hedin G, Li G, Nord CE. Genetic diversity of community-associated methicillin-resistant Staphylococcus aureus in southern Stockholm, 2000-2005. Clin Microbiol Infect 2008; 14: 370-376.
13. Denis O, Deplano A, De Beenhouwer H et al. Polyclonal emergence and importation of community-acquired methicillin-resistant Staphylococcus aureus strains harbouring Panton-Valentine leucocidin genes in Belgium. J Antimicrob Chemother 2005; 56: 1103-1106.
14. Otter JA, Havill NL, Boyce JM, French GL. Comparison of community-associated methicillin-resistant Staphylococcus aureus from teaching hospitals in London and the USA, 2004-2006: where is USA300 in the UK? Eur J Clin Microbiol Infect Dis 2009; 28: 835-839.
15. Jans B. Surveillance du MRSA dans les hôpitaux aigus belges, 2008. Available at: (last accessed 19 September 2011).
16. Lelievre H, Lina G, Jones ME et al. Emergence and spread in French hospitals of methicillin-resistant Staphylococcus aureus with increasing susceptibility to gentamicin and other antibiotics. J Clin Microbiol 1999; 37: 3452-3457.
17. Witte W, Braulke C, Cuny C, Heuck D, Kresken M. Changing pattern of antibiotic resistance in methicillin-resistant Staphylococcus aureus from German hospitals. Infect Control Hosp Epidemiol 2001; 22: 683-686.
18. Denis O, Deplano A, Nonhoff C et al. National surveillance of methicillin-resistant Staphylococcus aureus in Belgian hospitals indicates rapid diversification of epidemic clones. Antimicrob Agents Chemother 2004; 48: 3625-3629.
19. Maes N, Magdalena J, Rottiers S, De Gheldre Y, Struelens MJ. Evaluation of a triplex pcr assay to discriminate Staphylococcus aureus from coagulase-negative staphylococci and determine methicillin resistance from blood cultures. J Clin Microbiol 2002; 40: 1514-1517.
20. (CLSI) NCCLS. Performance standards for antimicrobial susceptibility testing. Twelfth information supplement. Wayne, PA: National Committee for Clinical Laboratory Standards, 2002.
21. Comité de l'Antibiogramme de la Société Française de Microbiologie, 2003. Available at: (last accessed 19 September 2011).
22. British Society for Antimicrobial Chemotherapy. BSAC methods for antimicrobial suceptibility testing, 2011. Available at: (last accessed 19 September 2011).
23. Dufour P, Gillet Y, Bes M et al. Community-acquired methicillin-resistant Staphylococcus aureus infections in France: emergence of a single clone that produces Panton-Valentine leukocidin. Clin Infect Dis 2002; 35: 819-824.
24. Deplano A, Witte W, Van Leeuwen WJ, Brun Y, Struelens MJ. Clonal dissemination of epidemic methicillin-resistant Staphylococcus aureus in Belgium and neighbouring countries. Clin Microbiol Infect 2000; 6: 239-245.
25. Denis O, Deplano A, De Ryck R, Nonhoff C, Struelens MJ. Emergence and spread of gentamicin-susceptible strains of methicillin-resistant Staphylococcus aureus in Belgian hospitals. Microb Drug Resist 2003; 9: 61-71.
26. Hallin M, Denis O, Deplano A et al. Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey. J Antimicrob Chemother 2007; 59: 465-472.
27. Kondo Y, Ito T, Ma XX et al. Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 2007; 51: 264-274.
28. Oliveira DC, de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2002; 46: 2155-2161.
29. Larsen AR, Bocher S, Stegger M, Goering R, Pallesen LV, Skov R. Epidemiology of European community-associated methicillin-resistant Staphylococcus aureus clonal complex 80 type iv strains isolated in Denmark from 1993 to 2004. J Clin Microbiol 2008; 46: 62-68.
30. Wertheim HF, Melles DC, Vos MC et al. The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis 2005; 5: 751-762.
31. Huang YC, Chao AS, Chang SD et al. Association of Staphylococcus aureus colonization in parturient mothers and their babies. Pediatr Infect Dis J 2009; 28: 742-744.
32. Peacock SJ, Justice A, Griffiths D et al. Determinants of acquisition and carriage of Staphylococcus aureus in infancy. J Clin Microbiol 2003; 41: 5718-5725.
33. Matussek A, Taipalensuu J, Einemo IM, Tiefenthal M, Lofgren S. Transmission of Staphylococcus aureus from maternity unit staff members to newborns disclosed through spa typing. Am J Infect Control 2007; 35: 122-125.
34. Stone A, Quittell L, Zhou J et al. Staphylococcus aureus nasal colonization among pediatric cf patients and their household contacts. Pediatr Infect Dis J 2009; 28: 895-899.
35. Vergison A, Denis O, Deplano A et al. National survey of molecular epidemiology of Staphylococcus aureus colonization in Belgian cystic fibrosis patients. J Antimicrob Chemother 2007; 59: 893-899.
36. Glikman D, Siegel JD, David MZ et al. Complex molecular epidemiology of methicillin-resistant Staphylococcus aureus isolates from children with cystic fibrosis in the era of epidemic community-associated methicillin-resistant S. aureus. Chest 2008; 133: 1381-1387.
37. Brook I, Gober AE. Bacteriology of spontaneously draining acute otitis media in children before and after the introduction of pneumococcal vaccination. Pediatr Infect Dis J 2009; 28: 640-642.
38. Kim ES, Song JS, Lee HJ et al. A survey of community-associated methicillin-resistant Staphylococcus aureus in Korea. J Antimicrob Chemother 2007; 60: 1108-1114.
39. McCarthy NL, Sullivan PS, Gaynes R, Rimland D. Health care-associated and community-associated methicillin-resistant Staphylococcus aureus infections: a comparison of definitions. Am J Infect Control 2010; 38: 600-606.
40. Ferry T, Bes M, Dauwalder O et al. Toxin gene content of the Lyon methicillin-resistant Staphylococcus aureus clone compared with that of other pandemic clones. J Clin Microbiol 2006; 44: 2642-2644.
41. Denis O, Deplano A, Nonhoff C et al. In vitro activities of ceftobiprole, tigecycline, daptomycin, and 19 other antimicrobials against methicillin-resistant Staphylococcus aureus strains from a national survey of Belgian hospitals. Antimicrob Agents Chemother 2006; 50: 2680-2685.
42. Kikuchi K, Takahashi N, Piao C, Totsuka K, Nishida H, Uchiyama T. Molecular epidemiology of methicillin-resistant Staphylococcus aureus strains causing neonatal toxic shock syndrome-like exanthematous disease in neonatal and perinatal wards. J Clin Microbiol 2003; 41: 3001-3006.
43. Jamart S, Denis O, Deplano A et al. Methicillin-resistant Staphylococcus aureus toxic shock syndrome. Emerg Infect Dis 2005; 11: 636-637.
44. Durand G, Bes M, Meugnier H et al. Detection of new methicillin-resistant Staphylococcus aureus clones containing the toxic shock syndrome toxin 1 gene responsible for hospital- and community-acquired infections in France. J Clin Microbiol 2006; 44: 847-853.
45. Moran GJ, Krishnadasan A, Gorwitz RJ et al. Methicillin-resistant S. aureus infections among patients in the emergency department. N Engl J Med 2006; 355: 666-674.