An Aspergillus oryzae acetyl xylan esterase: Molecular cloning and characteristics of recombinant enzyme expressed in Pichia pastoris

Journal of Biotechnology

Volumn 121, Issue 3, 2006, Pages 381-389

  Koseki, Takuya ^a,b   Miwa, Yozo ^b   Akao, Takeshi ^a   Akita, Osamu ^a   Hashizume, Katsumi ^a  

^a NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Acetyl xylan esterase; Aspergillus oryzae; Heterologous expression; Pichia pastoris

Indexed keywords

ACETIC ACID; AMINO ACIDS; CELL CULTURE; CLONING; ENZYME KINETICS; GENETIC ENGINEERING; PROTEINS;

ACETYL XYLAN ESTERASE; ASPERGILLUS ORYZAE; HETEROLOGOUS EXPRESSION; PICHIA PASTORIS;

ESTERS;

1 NAPHTHYL ACETATE; ACETIC ACID; AMINO ACID; ESTERASE; FUNGAL ENZYME; MATING HORMONE ALPHA FACTOR; SIGNAL PEPTIDE; XYLAN;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS ORYZAE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; EXPRESSED SEQUENCE TAG; HYDROLYSIS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; WESTERN BLOTTING;

ACETYLESTERASE; AMINO ACID SEQUENCE; ASPERGILLUS ORYZAE; BASE SEQUENCE; CATALYSIS; CLONING, MOLECULAR; CONSERVED SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; FUNGAL PROTEINS; GENETIC ENGINEERING; HYDROGEN-ION CONCENTRATION; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PICHIA; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE; TIME FACTORS;

ASPERGILLUS; ASPERGILLUS ORYZAE; PICHIA PASTORIS;

EID: 30944456517     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.07.015     Document Type: Article

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