Targeted single-neuron infection with rabies virus for transneuronal multisynaptic tracing

Journal of Neuroscience Methods

Volumn 209, Issue 2, 2012, Pages 367-370

  Nguyen, Tuan D ^a   Wirblich, Christoph ^b   Aizenman, Elias ^a   Schnell, Matthias J ^b   Strick, Peter L ^a,c,d   Kandler, Karl ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b THOMAS JEFFERSON UNIVERSITY   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

^d CENTER FOR THE NEURAL BASIS OF COGNITION   (United States)

Author keywords

Rabies; Retrograde labeling; Tract tracing; Transneuronal tracing; Transsynaptic tracing

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; FLUORESCENCE IMAGING; INFECTION; INOCULATION; NERVE CELL; NERVE CELL NETWORK; NEUROANATOMICAL TRACT TRACING; NONHUMAN; PRIORITY JOURNAL; RABIES VIRUS; RAT; SINGLE CELL ANALYSIS; SYNAPSE; VIRUS REPLICATION;

ANIMALS; CELLS, CULTURED; CEREBRAL CORTEX; EMBRYO, MAMMALIAN; GREEN FLUORESCENT PROTEINS; NEURONS; ORGAN CULTURE TECHNIQUES; RABIES VIRUS; RATS; RATS, SPRAGUE-DAWLEY; TRANSFECTION;

EID: 84864047404     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2012.06.019     Document Type: Article

References (15)

1. Aizenman E., Stout A.K., Hartnett K.A., Dineley K.E., McLaughlin B., Reynolds I.J. Induction of neuronal apoptosis by thiol oxidation: putative role of intracellular zinc release. J. Neurochem. 2000, 75:1878-1888.
2. Atanasiu P., Perrin P., Favre S., Sisman J. Glycosylation and isoelectric properties of complete and defective rabies viruses (author's transl). Ann. Microbiol. 1979, 130A:85-101.
3. Callaway E.M. Transneuronal circuit tracing with neurotropic viruses. Curr. Opin. Neurobiol. 2008, 18:617-623.
4. De Simoni A., Yu L.M. Preparation of organotypic hippocampal slice cultures: interface method. Nat. Protoc. 2006, 1:1439-1445.
5. Dietzschold B., Cox J.H., Schneider L.G., Wiktor T.J., Koprowski H. Isolation and purification of a polymeric form of the glycoprotein of rabies virus. J. Gen. Virol. 1978, 40:131-139.
6. Gomme E.A., Wanjalla C.N., Wirblich C., Schnell M.J. Rabies virus as a research tool and viral vaccine vector. Adv. Virus Res. 2011, 79:139-164.
7. Kelly R.M., Strick P.L. Rabies as a transneuronal tracer of circuits in the central nervous system. J. Neurosci. Methods 2000, 103:63-71.
8. Marshel J.H., Mori T., Nielsen K.J., Callaway E.M. Targeting single neuronal networks for gene expression and cell labeling in vivo. Neuron 2010, 67:562-574.
9. Rancz E.A., Franks K.M., Schwarz M.K., Pichler B., Schaefer A.T., Margrie T.W. Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics. Nat. Neurosci. 2011, 14:527-532.
10. Tan G.S., Preuss M.A., Williams J.C., Schnell M.J. The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription. Proc. Natl. Acad. Sci. U. S. A. 2007, 104:7229-7234.
11. Ugolini G. Advances in viral transneuronal tracing. J. Neurosci. Methods 2010, 194:2-20.
12. Ugolini G. Specificity of rabies virus as a transneuronal tracer of motor networks: transfer from hypoglossal motoneurons to connected second-order and higher order central nervous system cell groups. J. Comp. Neurol. 1995, 356:457-480.
13. Wickersham I.R., Finke S., Conzelmann K.K., Callaway E.M. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat. Methods 2007, 4:47-49.
14. Wickersham I.R., Lyon D.C., Barnard R.J., Mori T., Finke S., Conzelmann K.K., et al. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. Neuron 2007, 53:639-647.
15. Wirblich C., Schnell M.J. Rabies virus (RV) glycoprotein expression levels are not critical for pathogenicity of RV. J. Virol. 2011, 85:697-704.