Impaired osteoblastogenesis potential of progenitor cells in skeletal unloading is associated with alterations in angiogenic and energy metabolism profile

Bio-Medical Materials and Engineering

Volumn 22, Issue 4, 2012, Pages 219-226

  Vandamme, Katleen ^a,b   Holy, Xavier ^c   Bensidhoum, Morad ^a   Deschepper, Mickael ^a   Logeart Avramoglou, Delphine ^a   Naert, Ignace ^b   Duyck, Joke ^b   Petite, Hervé ^a  

^a UNIVERSITÉ PARIS DESCARTES   (France)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c INSTITUT DE RECHERCHE BIOMÉDICALE DES ARMÉES   (France)

Author keywords

differentiation; multi scale assessment; perfusion and energy metabolism; progenitor cells; Skeletal unloading

Indexed keywords

ADIPOGENIC; BONE FORMATION; BONE LOSS; BONE MARROW; BONE RESORPTION; BONE TISSUE; CELLULAR ENERGY; ENERGY METABOLISM; EX-VIVO; HIND LIMBS; METABOLIC STRESS; MICRO CT; MICROGRAVITY CONDITIONS; MULTISCALES; OSTEOBLASTIC ACTIVITY; OSTEOBLASTOGENESIS; OSTEOGENIC; OSTEOGENIC DIFFERENTIATION; PROGENITOR CELL; TRABECULAR ARCHITECTURE; UNDERLYING MECHANISM; VASCULARIZATION; WISTAR RAT;

ASSAYS; BIOELECTRIC POTENTIALS; CELL CULTURE; CELLS; COMPUTERIZED TOMOGRAPHY; DIFFERENTIATION (CALCULUS); METABOLISM; MICROGRAVITY; MICROGRAVITY PROCESSING; MUSCULOSKELETAL SYSTEM; POLYMERASE CHAIN REACTION; TISSUE; UNLOADING;

BONE;

RATTUS; RATTUS NORVEGICUS;

ANGIOGENESIS; ANIMAL; ARTICLE; BONE DEVELOPMENT; BONE MARROW; BONE MARROW CELL; CELL DIFFERENTIATION; CYTOLOGY; ENERGY METABOLISM; FEMALE; GENE EXPRESSION REGULATION; HINDLIMB; IMMOBILIZATION; LEG; METABOLISM; OSTEOBLAST; PHYSIOLOGY; RAT; STEM CELL; VASCULARIZATION; WISTAR RAT;

ANIMALS; BONE MARROW; BONE MARROW CELLS; CELL DIFFERENTIATION; ENERGY METABOLISM; FEMALE; GENE EXPRESSION REGULATION; HINDLIMB; HINDLIMB SUSPENSION; LEG BONES; NEOVASCULARIZATION, PHYSIOLOGIC; OSTEOBLASTS; OSTEOGENESIS; RATS; RATS, WISTAR; STEM CELLS;

EID: 84863958120     PISSN: 09592989     EISSN: None     Source Type: Journal    
DOI: 10.3233/BME-2012-0711     Document Type: Article

References (22)

1. S. Kontulainen, H. Sievänen, P. Kannus, M. Pasanen and I. Vuori, Effect of long-term impact-loading on mass, size, and estimated strength of humerus and radius of female racquet-sports players: a peripheral quantitative computed tomography study between young and old starters and controls, J. Bone Miner. Res. 18 (2003), 352-359. (Pubitemid 36125928)
2. L.F. Dietlein and R.S. Johnston, US manned space flight: the first twenty years, a biomedical status report, Acta Astronaut. 8 (1981), 893-906.
3. G. Carmeliet, G. Nys, I. Stockmans and R. Bouillon, Gene expression related to the differentiation of osteoblastic cells is altered by microgravity, Bone 22 (1998), 139S-143S. (Pubitemid 28223273)
4. L. Vico, P. Collet, A. Guignandon, M.-H. Lafage-Proust, T. Thomas, M. Rehaillia and C. Alexandre, Effects of long-term microgravity exposure on cancellous and cortical weight-bearing bones of cosmonauts, Lancet 355 (2000), 1607-1611. (Pubitemid 30240506)
5. E.R. Morey-Holton and R.K. Globus, Hindlimb unloading of growing rats: a model for predicting skeletal changes during space flight, Bone 22 (1998), 83S-88S. (Pubitemid 28223261)
6. O. Barou, D. Valentin, L. Vico, C. Tirode, A. Barbier, C. Alexandre and M.-H. Lafage-Proust, High-resolution threedimensional micro-computed tomography detects bone loss and changes in trabecular architecture early: comparison with DEXA and bone histomorphometry in a rat model of disuse osteoporosis, Invest. Radiol. 37 (2002), 40-46. (Pubitemid 34027844)
7. C. Dufour, X. Holy and P.J. Marie, Skeletal unloading induces osteoblast apoptosis and targets alpha5beta1-PI3K-Bcl-2 signaling in rat bone, Exp. Cell Res. 313 (2007), 394-403. (Pubitemid 46048823)
8. V. David, N. Laroche, B. Boudignon, M.-H. Lafage-Proust, C. Alexandre, P. Ruegsegger and L. Vico, Noninvasive in vivo monitoring of bone architecture alterations in hindlimb-unloaded female rats using novel three-dimensional microcomputed tomography, J. Bone Miner. Res. 18 (2003), 1622-1631. (Pubitemid 37022920)
9. P.J. Kostenuik, B.P. Halloran, E.R. Morey-Holton and D.D. Bikle, Skeletal unloading inhibits the in vitro proliferation and differentiation of rat osteoprogenitor cells, Am. J. Physiol. 273 (1997), E1133-E1139.
10. M. Machwate, E. Zerath, X. Holy, M. Hott, D. Modrowski, A. Malouvier and P.J. Marie, Skeletal unloading in rat decreases proliferation of rat bone and marrow-derived osteoblastic cells, Am. J. Physiol. 264 (1993), E790-E799. (Pubitemid 23156755)
11. D.D. Bikle, J. Harris, B.P. Halloran and E.R. Morey-Holton, Altered skeletal pattern of gene expression in response to spaceflight and hindlimb elevation, Am. J. Physiol. 267 (1994), E822-E827.
12. R. Zhang, S.C. Supowit, G.L. Klein, Z. Lu, M.D. Christensen, R. Lozano and D.J. Simmons, Rat tail suspension reduces messenger RNA level for growth factors and osteopontin and decreases the osteoblastic differentiation of bone marrow stromal cells, J. Bone Miner. Res. 10 (1995), 415-423.
13. P.N. Colleran, M.K. Wilkerson, S.A. Bloomfield, L.J. Suva, R.T. Turner and M.D. Delp, Alterations in skeletal perfusion with simulated microgravity: a possible mechanism for bone remodeling, J. Appl. Physiol. 89 (2000), 1046-1054. (Pubitemid 32125404)
14. X. Holy and E. Zerath, The suspension device for hindlimb unloading in the rat-results of a video monitoring study, STAL 21 (1996), 177-184. (Pubitemid 126821406)
15. C.A. McCulloch, M. Strugurescu, F. Hughes, A.H. Melcher and J.E. Aubin, Osteogenic progenitor cells in rat bone marrow stromal populations exhibit self-renewal in culture, Blood 77 (1991), 1906-1911.
16. E. Roudier, C. Gineste, A. Wazna, K. Dehghan, D. Desplanches and O. Birot, Angio-adaptation in unloaded skeletal muscle: new insights into an early and muscle type-specific dynamic process, J. Physiol. 588 (2010), 4579-4591.
17. A. Wagatsuma, Effect of hindlimb unweighting on expression of hypoxia-inducible factor-1alpha vascular endothelial growth factor, angiopoietin, and their receptors in mouse skeletal muscle, Physiol. Res. 57 (2008), 613-620.
18. J.A. Reihill, M.A. Ewart, D.G. Hardie and I.P. Salt, AMP-activated protein kinase mediates VEGF-stimulated endothelial NO production, Biochem. Biophys. Res. Commun. 354 (2007), 1084-1088. (Pubitemid 46216246)
19. G.R. Steinberg and B.C. Kemp, AMPK in health and disease, Physiol. Rev. 89 (2009), 1025-1078.
20. D.D. Bikle, B.P. Halloran, C.M. Cone, R.K. Globus and E.R. Morey-Holton, The effects of simulated weightlessness on bone maturation, Endocrinology 120 (1987), 678-684. (Pubitemid 17020423)
21. S.R. Shaw, A.C. Vailas, R.E. Grindeland and R.F. Zernicke, Effects of a 1-wk spaceflight on morphological and mechanical properties of growing bone, Am. J. Physiol. 254 (1988), R78-R83.
22. Z. Pan, J. Yang, C. Guo, D. Shi, D. Shen, Q. Zheng, R. Chen, Y. Xu, Y. Xi and J. Wang, Effects of hindlimb unloading on ex vivo growth and osteogenic/adipogenic potentials of bone marrow-derived mesenchymal stem cells in rats, Stem Cells Dev. 17 (2008), 795-804.