Engineering an improved IgG4 molecule with reduced disulfide bond heterogeneity and increased fab domain thermal stability

Journal of Biological Chemistry

Volumn 287, Issue 29, 2012, Pages 24525-24533

  Peters, Shirley J ^a,b   Smales, C Mark ^b   Henry, Alistair J ^a   Stephens, Paul E ^a   West, Shauna ^a   Humphreys, David P ^a  

^a UCB PHARMA   (Belgium)

^b UNIVERSITY OF KENT   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY STRUCTURES; BIOPHYSICAL CHARACTERIZATION; C TERMINUS; CHINESE HAMSTER OVARY CELLS; DISULFIDE BONDS; HEAVY CHAIN; IMMUNOBLOTS; LIGHT CHAIN; REGULATORY AUTHORITIES; TRANSIENT EXPRESSION;

COVALENT BONDS; MOLECULES; THERMODYNAMIC STABILITY;

ANTIBODIES;

CYSTEINE; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G4; SERINE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHO CELL; DISULFIDE BOND; FEMALE; GENE MUTATION; IMMUNOBLOTTING; IMMUNOGLOBULIN PRODUCTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN POLYMORPHISM; PROTEIN PURIFICATION; PROTEIN STABILITY; SUPERNATANT; THERMOSTABILITY; TRANSIENT EXPRESSION;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CHO CELLS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; DISULFIDES; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN ENGINEERING; PROTEIN STABILITY; SEQUENCE HOMOLOGY, AMINO ACID;

CRICETULUS GRISEUS;

EID: 84863795910     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.369744     Document Type: Article

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