New markers for old stains: Stable mRNA markers for blood and saliva identification from up to 16-year-old stains

International Journal of Legal Medicine

Volumn 123, Issue 1, 2009, Pages 71-74

  Zubakov, Dmitry ^a   Kokshoorn, Mieke ^a   Kloosterman, Ate ^b   Kayser, Manfred ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b NETHERLANDS FORENSIC INSTITUTE   (Netherlands)

Author keywords

Blood; Body fluid; Cellular origin; MRNA markers; RNA stability; Saliva

Indexed keywords

MESSENGER RNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; BLOOD STAIN; FORENSIC GENETICS; GENE EXPRESSION PROFILING; GENETIC MARKER; HUMAN; ISOLATION AND PURIFICATION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALIVA; TIME;

BLOOD STAINS; ELECTROPHORESIS, AGAR GEL; FORENSIC GENETICS; GENE EXPRESSION PROFILING; GENETIC MARKERS; HUMANS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SALIVA; TIME FACTORS;

EID: 66749104582     PISSN: 09379827     EISSN: 14371596     Source Type: Journal    
DOI: 10.1007/s00414-008-0249-z     Document Type: Article

References (8)

1. van Hoof A, Parker R (2002) Messenger RNA degradation: beginning at the end. Curr Biol 12:285-287
2. Bauer M, Kraus A, Patzelt D (1999) Detection of epithelial cells in dried blood stains by reverse transcriptase-polymerase chain reaction. J Forensic Sci 44:1232-1236
3. Juusola J, Ballantyne J (2003) Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification. Forensic Sci Int 135(2):85-96
4. Alvarez M, Juusola J, Ballantyne J (2004) An mRNA and DNA co-isolation method for forensic casework samples. Anal Biochem 335(2):289-298
5. Juusola J, Ballantyne J (2005) Multiplex mRNA profiling for the identification of body fluids. Forensic Sci Int 152(1):1-12
6. Heinrich M, Matt K, Lutz-Bonengel S, Schmidt U (2007) Successful RNA extraction from various human postmortem tissues. Int J Legal Med 121:136-142
7. Karlsson H, Guthenberg C, von Dobeln U, Kristenssson K (2003) Extraction of RNA from dried blood on filter papers after long-term storage. Clin Chem 49:979-981
8. Zubakov D, Hanekamp E, Kokshoorn M, van IJcken W, Kayser M (2008) Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of timewise degraded samples. Int J Legal Med 122(2):135-142