Evaluation of rapid mecA gene detection versus standard culture in staphylococcal chronic prosthetic joint infections

Diagnostic Microbiology and Infectious Disease

Volumn 73, Issue 4, 2012, Pages 318-321

  Titécat, Marie ^a   Loïez, Caroline ^a   Senneville, Eric ^a,b,d   Wallet, Frédéric ^a   Dezèque, Hervé ^a,c,d   Legout, Laurence ^a,b,d   Migaud, Henri ^a,c,d   Courcol, René J ^a  

^a LILLE UNIVERSITY HOSPITAL   (France)

^b UNIV LILLE   (France)

^c Dron Hospital   (France)

^d Centre de Reference des Infections Osteo Articulaires Complexes Nord Ouest Lille   (France)

Author keywords

Chronic prosthetic joint infections; MecA; Rapid detection

Indexed keywords

VANCOMYCIN;

ADULT; AGED; ANTIBIOTIC THERAPY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BIOASSAY; CLINICAL ARTICLE; FEMALE; GENETIC IDENTIFICATION; HIP PROSTHESIS; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; KNEE PROSTHESIS; LABORATORY DEVICE; MALE; MECA GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS INFECTION; NONHUMAN; PERIPROSTHETIC JOINT INFECTION; PREDICTIVE VALUE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS;

AGED; AGED, 80 AND OVER; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; COHORT STUDIES; FEMALE; HUMANS; MALE; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; OSTEOARTHRITIS; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; PROSTHESIS-RELATED INFECTIONS; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; TIME FACTORS; VANCOMYCIN;

EID: 84863774734     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.04.011     Document Type: Article

References (16)

1. Gould I.M., Cauda R., Esposito S., Gudiol F., Mazzei T., Garau J. Management of serious methicillin-resistant Staphylococcus aureus infections: what are the limits?. Int J Antimicrob Agents 2011, 37:202-209.
2. Kim S.H., Kim K.H., Kim H.B., Kim N.J., Kim E.C., Oh M.D., et al. Outcome of vancomycin treatment in patients with methicillin-susceptible Staphylococcus aureus bacteremia. Antimicrob Agents Chemother 2008, 52:192-197.
3. Kobayashi N., Inaba Y., Choe H., Iwamoto N., Ishida T., Yukizawa Y., et al. Rapid and sensitive detection of methicillin-resistant Staphylococcus periprosthetic infections using real-time polymerase chain reaction. Diagn Microbiol Infect Dis 2009, 64:172-176.
4. Kurtz S.M., Lau E., Schmier J., Ong K.L., Zhao K., Parvizi J. Infection burden for hip and knee arthroplasty in the United States. J Arthroplasty 2008, 23:984-991.
5. Nguyen D.T., Yeh E., Perry S., Luo R.F., Pinsky B.A., Lee B.P., et al. Real-time PCR testing for mecA reduces vancomycin usage and length of hospitalization for patients infected with methicillin-sensitive staphylococci. J Clin Microbiol 2010, 48:785-790.
6. Parta M., Goebel M., Thomas J., Matloobi M., Stager C., Musher D.M. Impact of an assay that enables rapid determination of Staphylococcus species and their drug susceptibility on the treatment of patients with positive blood culture results. Infect Control Hosp Epidemiol 2010, 31:1043-1048.
7. Rossney A.S., Herra C.M., Brennan G.I., Morgan P.M., O'Connell B. Evaluation of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens. J Clin Microbiol 2008, 46:3285-3290.
8. Roux A.L., Sivadon-Tardy V., Bauer T., Lortat-Jacob A., Herrmann J.L., Gaillard J.L., et al. Diagnosis of prosthetic joint infection by beadmill processing of a periprosthetic specimen. Clin Microbiol Infect 2011, 17:447-450.
9. Scanvic A., Courdavault L., Sollet J.P., Le Turdu F. Interest of real-time PCR Xpert MRSA/SA on GeneXpert® DX System in the investigation of staphylococcal bacteremia. Pathol Biol 2011, 59:67-72.
10. Senneville E., Savage C., Nallet I., Yazdanpanah Y., Giraud F., Migaud H., et al. Improved aero-anaerobe recovery from infected prosthetic joint samples taken from 72 patients and collected intraoperatively in Rosenow's broth. Acta Orthop 2006, 77:120-124.
11. Recommendations for bone and joint prosthetic device infections in clinical practice (prosthesis, implants, osteosynthesis) Société de Pathologie Infectieuse de Langue Francaise (SPILF). http://www.infectiologie.com/site/medias/_documents/consensus/inf-osseuse-long.pdf.
12. Trampuz A., Zimmerli W. Diagnosis and treatment of implant-associated septic arthritis and osteomyelitis. Curr Infect Dis Rep 2008, 10:394-403.
13. Wolk D.M., Marx J.L., Dominguez L., Driscoll D., Schifman R.B. Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in nares: diagnostic accuracy for surveillance samples with various bacterial densities. J Clin Microbiol 2009, 47:3933-3936.
14. Wolk D.M., Picton E., Johnson D., Davis T., Pancholi P., Ginocchio C.C., et al. Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares. J Clin Microbiol 2009, 47:758-764.
15. Wolk D.M., Struelens M.J., Pancholi P., Davis T., Della-Latta P., Fuller D., et al. Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays. J Clin Microbiol 2009, 47:823-826.
16. Zimmerli W., Trampuz A., Ochsner P.E. Prosthetic-joint infections. N Engl J Med 2004, 351:1645-1654.