Gene overexpression and gene silencing in birch using an agrobacterium-mediated transient expression system

Molecular Biology Reports

Volumn 39, Issue 5, 2012, Pages 5537-5541

  Zhang, Yan ^a   Wang, Yucheng ^a   Wang, Chao ^a  

^a NORTHEAST FORESTRY UNIVERSITY   (China)

Author keywords

Agrobacterium; Betula platyphylla Suk; CCR; Silencing; Transient gene expression

Indexed keywords

GREEN FLUORESCENT PROTEIN; ALDEHYDE DEHYDROGENASE; BETA GLUCURONIDASE; CINNAMOYL COA REDUCTASE;

ARTICLE; BETULA PLATYPHYLLA; BIRCH; CINNAMOYL COA REDUCTASE GENE; CONTROLLED STUDY; GENE; GENE IDENTIFICATION; GENE OVEREXPRESSION; GENE SILENCING; GENETIC TRANSFORMATION; HISTOCHEMISTRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; REAL TIME POLYMERASE CHAIN REACTION; RHIZOBIUM; RNA INTERFERENCE; RNA SEQUENCE; SEEDLING; SEQUENCE ANALYSIS; TRANSIENT EXPRESSION; ENZYMOLOGY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; TRANSGENIC PLANT;

AGROBACTERIUM; BETULA PLATYPHYLLA;

AGROBACTERIUM; ALDEHYDE OXIDOREDUCTASES; BETULA; GENE EXPRESSION; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; IMMUNOHISTOCHEMISTRY; MOLECULAR BIOLOGY; PLANTS, GENETICALLY MODIFIED; REAL-TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

EID: 84863753094     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-1357-2     Document Type: Article

References (19)

1. Koiwa H, Bressan RA, Hasegawa PM (2006) Identification of plant stress-responsive determinants in Arabidopsis by largescale forward genetic screens. J Exp Bot 57(5):1119-1128
2. Spokevicius AV, Van Beveren K, Leitch MA, Bossinger G (2005) Agrobacterium-mediated in vitro transformation of wood-producing stem segments in eucalypts. Plant Cell Rep 23(9):617-624
3. Bhaskar PB, Venkateshwaran M, Wu L, Ané JM, Jiang J (2009) Agrobacterium-mediated transient gene expression and silencing: a rapid tool for functional gene assay in potato. PLoS ONE 4(6):e5812
4. Li JF, Park E, von Arnim AG, Nebenführ A (2009) The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. Plant Methods 5:6
5. Aronen T, Hohtola A, Laukkanen H, Häggman H (1995) Seasonal changes in the transient expression of a 35S CaMV-GUS gene construct introduced into Scots pine buds. Tree Physiol 15(1):65-70
6. Ellis DD, McCabe D, Russell D, Martinell B, McCown BH (1991) Expression of inducible angiosperm promoters in a gymnosperm, Picea glauca (white spruce). Plant Mol Biol 17(1): 19-27
7. Qiao J, Ishihara Y, Kuroda H, Sakai F, Sakai H, Komano T (1997) Transient expression of goat growth hormone gene in poplar (Populus alba L.) protoplasts: a quick method for detection of foreign gene expression in mRNA level. Biosci Biotechnol Biochem 61(9):1580-1581
8. Rueda-López M, Crespillo R, Cánovas FM, Avila C (2008) Differential regulation of two glutamine synthetase genes by a single Dof transcription factor. Plant J 56(1):73-85
9. Wang C, Wang YC, Diao GP, Jiang J, Yang CP (2009) Isolation and characterization of expressed sequence tags (ESTs) from cambium tissue of Birch (Betula platyphylla Suk). Plant Mol Biol Rep 28(3):438-449
10. Kerschen A, Napoli CA, Jorgensen RA, Müller AE (2004) Effectiveness of RNA interference in transgenic plants. FEBS Lett 566(1-3):223-228
11. Kenneth JL, Thomas DS (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDC T method. Methods 25(4):402-408
12. Li JF, Nebenführ A (2010) FAST technique for Agrobacteriummediated transient gene expression in seedlings of Arabidopsis and other plant species. Cold Spring Harb Protoc 2010(5): pdb.prot5428
13. Koscianska E, Kalantidis K, Wypijewski K, Sadowski J, Tabler M (2005) Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum. Plant Mol Biol 59:647-661
14. Wang HZ, Chen YP, Chen PD (2007) Plant transient expression system in functional genomics. Sheng Wu Gong Cheng Xue Bao 23(3):367-374
15. Mohanpuria P, Rana NK, Yadav SK (2008) Transient RNAi based gene silencing of glutathione synthetase reduces glutathione content in Camellia sinensis (L.) O. Kuntze somatic embryos. Biol Plant 52(2):381-384
16. Douchkov D, Nowara D, Zierold U, Schweizer P (2005) A highthroughput gene-silencing system for the functional assessment of defense-related genes in barley epidermal cells. Mol Plant Microbe Interact 18(8):755-761
17. Smith NA, Singh SP, Wang MB, Stoutjesdijk PA, Green AG, Waterhouse PM (2000) Total silencing by intron spliced hairpin RNAs. Nature 407(6802):319-320
18. Voinnet O, Rivas S, Mestre P, Baulcombe D (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33(5):949-956
19. Johansen LK, Carrington JC (2001) Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium- mediated transient expression system. Plant Physiol 126(3): 930-938