FAST technique for agrobacterium-mediated transient gene expression in seedlings of Arabidopsis and other plant species

Cold Spring Harbor Protocols

Volumn 5, Issue 5, 2010, Pages

  Li, Jian Feng ^a,b   Nebenführ, Andreas ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SURFACTANT;

ADULT PLANT; ARABIDOPSIS; ARTICLE; BACTERIUM COLONY; BIOASSAY; CELL TRANSFORMATION; CELLULAR DISTRIBUTION; CENTRIFUGATION; CLINICAL PROTOCOL; COCULTURE; CULTURE MEDIUM; FAST AGROBACTERIUM MEDIATED SEEDLING TRANSFORMATION TECHNIQUE; GENE FUNCTION; GENETIC PROCEDURES; GERMINATION; NONHUMAN; PLANT DEVELOPMENT; PLANT GENE; PLANT GENETICS; PLANT GROWTH; PROCESS DESIGN; PROTEIN PROTEIN INTERACTION; RHIZOBIUM RADIOBACTER; SEEDLING; TRANSGENIC PLANT; TRANSIENT EXPRESSION;

AGROBACTERIUM; ARABIDOPSIS;

EID: 77956270893     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5428     Document Type: Article

References (4)

1. Campanoni P, Sutter JU, Davis CS, Littlejohn GR, Blatt MR. 2007. A generalized method for transfecting root epidermis uncovers endosomal dynamics in Arabidopsis root hairs. Plant J 51: 322-330.
2. L-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector. Mol Gen Genet 204: 383-396.
3. Li JF, Park E, von Arnim AG, Nebenführ A. 2009. The FAST technique: A simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. Plant Methods 5: 6.
4. Marion J, Bach L, Bellec Y, Meyer C, Gissot L, Faure JD. 2008. Systematic analysis of protein subcellular localization and interaction using high-throughput transient transformation of Arabidopsis seedlings. Plant J 56: 169-179.