Efficient differentiation of mouse embryonic stem cells into motor neurons

Journal of Visualized Experiments

Volumn , Issue 64, 2012, Pages

  Wu, Chia Yen ^a   Whye, Dosh ^a   Mason, Robert W ^a   Wang, Wenlan ^a  

^a NEMOURS ALFRED I DUPONT HOSPITAL FOR CHILDREN   (United States)

Author keywords

Choline acetyltransferase; Developmental biology; Hb9; Islet 1; Issue 64; Molecular biology; Motor neurons; Mouse embryonic stem cells; Neurosciences; Retinoic acid; Sonic hedgehog; Spinal cord; Stem cell biology

Indexed keywords

CARRIER PROTEIN; CHOLINE ACETYLTRANSFERASE; FIBROBLAST GROWTH FACTOR 2; FIBROBLAST GROWTH FACTOR 8; G PROTEIN COUPLED RECEPTOR; GREEN FLUORESCENT PROTEIN; INSULIN GENE ENHANCER BINDING PROTEIN ISL 1; INSULIN GENE ENHANCER BINDING PROTEIN ISL-1; LIM HOMEODOMAIN PROTEIN; NOGGIN PROTEIN; RETINOIC ACID; SONIC HEDGEHOG PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL DIFFERENTIATION; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; DRUG POTENTIATION; EMBRYONIC STEM CELL; FLUORESCENT ANTIBODY TECHNIQUE; METABOLISM; METHODOLOGY; MOTONEURON; MOUSE; TRANSGENIC MOUSE;

ANIMALS; CARRIER PROTEINS; CELL DIFFERENTIATION; CHOLINE O-ACETYLTRANSFERASE; CYTOLOGICAL TECHNIQUES; EMBRYONIC STEM CELLS; FIBROBLAST GROWTH FACTOR 2; FIBROBLAST GROWTH FACTOR 8; FLUORESCENT ANTIBODY TECHNIQUE; GREEN FLUORESCENT PROTEINS; HEDGEHOG PROTEINS; LIM-HOMEODOMAIN PROTEINS; MICE; MICE, TRANSGENIC; MOTOR NEURONS; RECEPTORS, G-PROTEIN-COUPLED; TRANSCRIPTION FACTORS; TRETINOIN;

EID: 84863732129     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3813     Document Type: Article

References (12)

1. Wichterle, H., Lieberam, I., Porter, J.A., & Jessell, T. M. Directed differentiation of embryonic stem cells into motor neurons. Cell. 110, 385-397 (2002).
2. Miles, G.B., et al. Functional properties of motoneurons derived from mouse embryonic stem cells. J. Neurosci. 24, 7848-7858 (2004).
3. Wichterle, H. & Peljto, M. Differentiation of mouse embryonic stem cells to spinal motor neurons. Curr. Protoc. Stem Cell. Biol. Chapter 1, Unit 1H.1.1-1H.1.9 (2008).
4. Kiris, E., et al. Embryonic stem cell-derived motoneurons provide a highly sensitive cell culture model for botulinum neurotoxin studies, with implications for high-throughput drug discovery. Stem Cell Res. 6, 195-205 (2011).
5. Wu, C.Y. et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci. 12, 25 (2011).
6. McMahon, J.A., et al. Noggin-mediated antagonism of BMP signaling is required for growth and patterning of the neural tube and somite. Genes Dev. 12, 1438-1452 (1998).
7. Storey, K.G., et al. Early posterior neural tissue is induced by FGF in the chick embryo. Development. 125, 473-484 (1998).
8. Launay, C., Fromentoux, V., Shi, D.L., & Boucaut, J. C. A truncated FGF receptor blocks neural induction by endogenous Xenopus inducers. Development. 122, 869-880 (1996)
9. Lamb, T.M. & Harland, R.M. Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anterior-posterior neural pattern. Development. 121, 3627-3636 (1995).
10. Sinha, S. & Chen, J.K. Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat. Chem. Biol. 2, 29-30 (2006).
11. Chen, J. K., Taipale, J., Young, K. E., Maiti, T. & Beachy, P.A. Small molecule modulation of Smoothened activity. Proc. Natl. Acad. Sci. U.S.A. 99, 14071-14076 (2002).
12. Lee, S. M., Danielian, P.S., Fritzsch, B. & McMahon, A.P. Evidence that FGF8 signaling from the midbrain-hindbrain junction regulates growth and polarity in the developing midbrain. Development. 124, 959-969 (1997).