Purification of papain using reactive green 5 attached supermacroporous monolithic cryogel

Applied Biochemistry and Biotechnology

Volumn 167, Issue 3, 2012, Pages 552-563

  Uygun, Deniz Aktaş ^a   Akduman, Begüm ^a   Uygun, Murat ^a   Akgöl, Sinan ^b   Denizli, Adil ^c  

^a ADNAN MENDERES UNIVERSITY   (Turkey)

^b EGE UNIVERSITY   (Turkey)

^c HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Carica papaya; Cryogel; Keywords Dye ligand; Papain; Reactive green 5

Indexed keywords

CARICA PAPAYA; CRYOGELS; DYE-LIGANDS; PAPAIN; REACTIVE GREEN 5;

ADSORPTION; ELECTROPHORESIS; FOURIER TRANSFORM INFRARED SPECTROSCOPY; PHEMA; SCANNING ELECTRON MICROSCOPY; SODIUM; SODIUM SULFATE; SUBSTITUTION REACTIONS;

PURIFICATION;

CROSS LINKING REAGENT; DYE; N,N' METHYLENEBISACRYLAMIDE; NUCLEOPHILE; PAPAIN; POLYMACON; REACTIVE GREEN 5; UNCLASSIFIED DRUG;

ADSORPTION; ARTICLE; CRYOGEL; ENERGY DISPERSIVE X RAY ANALYSIS; ENZYME PURIFICATION; INFRARED SPECTROSCOPY; NONHUMAN; PAPAYA; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERIZATION; POROSITY; RADICAL CRYOCOPOLYMERIZATION; SCANNING ELECTRON MICROSCOPY; SILVER STAINING; SUBSTITUTION REACTION; X RAY ANALYSIS;

ADSORPTION; BUFFERS; CARICA; CHEMICAL FRACTIONATION; COLORING AGENTS; CRYOGELS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; LISSAMINE GREEN DYES; PAPAIN; POLYAMINES; POLYHYDROXYETHYL METHACRYLATE; POROSITY; SILVER STAINING; WATER;

CARICA PAPAYA;

EID: 84863720129     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9707-7     Document Type: Article

References (42)

1. Sumantha, A., Larroche, C., & Pandey, A. (2006). Food Technology and Biotechnology, 44, 211-220.
2. Ghosh, S. (2005). Colloids and Surfaces A, 264, 6-16.
3. Vasconcellos, F. C., Goulart, G. A. S., & Beppu, M. M. (2011). Powder Technology, 205, 65-70.
4. Homaei, A. A., Sajedi, R. H., Sariri, R., Seyfzadeh, S., & Stevanato, R. (2010). Amino Acids, 38, 937-942.
5. Chen, T. X., Nie, H. L., Li, S. B., White, C. B., Su, S. N., & Zhu, L. M. (2009). Colloids and Surfaces B, 72, 25-31.
6. Kimmel, J. R., & Smith, E. L. (1954). Journal of Biological Chemistry, 207, 515-531.
7. Finkle, B. J., & Smith, E. L. (1958). Journal of Biological Chemistry, 230, 669-690.
8. Baines, B. S., & Brocklehurst, K. (1979). Biochemical Journal, 177, 541-548.
9. Nitsawang, S., Kaul, R. H., & Kanasawud, P. (2006). Enzyme and Microbial Technology, 39, 1103-1107.
10. Tombaccini, D., Mocali, A., Weber, E., & Paoletti, F. (2001). Analytical Biochemistry, 289, 231-238.
11. Govrin, E., & Levine, A. (1999). Protein Expression and Purification, 15, 247-250.
12. Nie, H. L., Chen, T. X., & Zhu, L. M. (2007). Separation and Purification Technology, 57, 121-125.
13. Yavuz, H., Duru, E., Genç, Ó;., & Denizli, A. (2003). Colloids and Surfaces A, 223, 185-193.
14. Yavuz, H., Akgöl, S., Say, R., & Denizli, A. (2006). International Journal of Biological Macromolecules, 39, 303-309.
15. Demiryas, N., Tüzmen, N., Galaev, I. Y., Pişkin, E., & Denzili, A. (2007). Journal of Applied Polymer Science, 105, 1808-1816.
16. Akgöl, S., Yavuz, H., Şenel, S., & Denizli, A. (2003). Reactive and Functional Polymers, 55, 45-51.
17. Altintaş, E. B., & Denizli, A. (2006). International Journal of Biological Macromolecules, 38, 99-106.
18. Wongchuphan, R., Tey, B. T., Tan, W. S., Taip, F. S., Kamal, S. M. M., & Ling, T. C. (2009). Biochemical Engineering Journal, 45, 232-238.
19. Lozinsky, V. I., Plieva, F. M., Galaev, I. Y., & Mattiasson, B. (2002). Bioseparation, 10, 163-188.
20. Lozinsky, V. I., Galaev, I. Y., Plieva, F. M., Savina, I. N., Jungvid, H., & Mattiasson, B. (2003). Trends in Biotechnology, 21, 445-451.
21. Plieva, F. M., Galaev, I. Y., & Mattiasson, B. (2007). Journal of Separation Science, 30, 1657-1671.
22. Avcibaşi, N., Uygun, M., Çorman, M. E., Akgöl, S., & Denizli, A. (2010). Applied Biochemistry and Biotechnology, 162, 2232-2243.
23. Derazshamshir, A., Baydemir, G., Andaç, M., Say, R., Galaev, I. Y., & Denizli, A. (2010). Macromolecular Chemistry and Physics, 6, 657-668.
24. Alkan, H., Bereli, N., Baysal, Z., & Denizli, A. (2009). Biochemical Engineering Journal, 45, 201-208.
25. Bereli, N., Andaç, M., Baydemir, G., Say, R., Galaev, I. Y., & Denizli, A. (2008). Journal of Chromatography. A, 1190, 18-26.
26. Yilmaz, F., Bereli, N., Yavuz, H., & Denizli, A. (2009). Biochemical Engineering Journal, 43, 272-279.
27. Bereli, N., Şener, G., Altindaş, E. B., Yavuz, H., & Denizli, A. (2010). Materials Science and Engineering: C-Mater, 30, 323-329.
28. Nie, H. L., & Zhu, L. M. (2007). International Journal of Biological Macromolecules, 40, 261-267.
29. Nie, H. L., Chen, T. X., & Zhu, L. M. (2007). Separation and Purification Technology, 57, 121-125.
30. Su, S. N., Nie, H. L., Zhu, L. M., & Chen, T. X. (2009). Bioresource Technology, 100, 2336-2340.
31. Perçin, I., Saĝlar, E., Yavuz, H., Aksöz, E., & Denizli, A. (2011). International Journal of Biological Macromolecules, 48, 577-582.
32. Bradford, M. M. (1976). Analytical Biochemistry, 72, 248-254.
33. Kembhavi, A. A., Kulkarni, A., & Pant, A. (1993). Applied Biochemistry and Biotechnology, 38, 83-92.
34. Laemmli, U. K. (1970). Nature, 227, 680-685.
35. Suster, C. R. L., Priolo, N. S., & Morcelle, S. R. (2011). Preparative Biochemistry and Biotechnology, 41, 107-121.
36. Monti, R., Basilio, C. A., Trevisan, H. C., & Contiero, J. (2000). Brazilian Archives of Biology and Technology, 43, 501-507.
37. Rauf, M. A., Bukallah, S. B., Hamour, F. A., & Nasir, A. S. (2008). Chemical Engineering Journal, 137, 238-243.
38. Karataş, M., Akgöl, S., Yavuz, H., Say, R., & Denizli, A. (2007). International Journal of Biological Macromolecules, 40, 254-260.
39. Burke, D. E., Lewis, S. D., & Shafer, J. A. (1974). Archives of Biochemistry and Biophysics, 164, 30-36.
40. Azarkan, M., El Moussaoui, A., van Wuytswinkel, D., Dehon, G., & Looze, Y. (2003). Journal of Chromatography B, 790, 229-238.
41. Nitsawang, S., Hatti-Kaul, R., & Kanasawud, P. (2006). Enzyme and Microbial Technology, 39, 1103-1107.
42. Llerena-Suster, C. R., Priolo, N. S., & Morcelle, S. R. (2011). Preparative Biochemistry and Biotechnology, 41, 107-121.