Pre-steady-state kinetic analysis of 1-deoxy-d-xylulose-5-phosphate reductoisomerase from mycobacterium tuberculosis reveals partially rate-limiting product release by parallel pathways

Biochemistry

Volumn 51, Issue 26, 2012, Pages 5307-5319

  Liu, Juan ^a,b   Murkin, Andrew S ^a  

^a UNIVERSITY AT BUFFALO   (United States)

^b UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINARY COMPLEXES; DRUG DISCOVERY; FLUORESCENCE MEASUREMENTS; FREE ENZYME; GLOBAL KINETICS; ISOPRENOIDS; KINETIC ISOTOPE EFFECTS; KINETIC MECHANISM; MECHANISTIC STUDIES; MYCOBACTERIUM TUBERCULOSIS; NONMEVALONATE PATHWAY; PARALLEL PATHWAYS; PRE-STEADY-STATE KINETIC ANALYSIS; PRODUCT INHIBITION; PRODUCT RELEASE; RATE LIMITING; RATE-LIMITING STEPS; REDUCTION REACTION; REDUCTOISOMERASE; STOPPED FLOW; SUBSTRATE BINDING; TERNARY COMPLEX; TRANSIENT KINETICS;

BIOCHEMISTRY; ESCHERICHIA COLI; FLUORESCENCE; ISOTOPES; REACTION KINETICS; TUBES (COMPONENTS);

KINETICS;

1 DEOXY DEXTRO XYLULOSE 5 PHOSPHATE REDUCTOISOMERASE; 2 METHYL DEXTRO ERYTHRITOL 4 PHOSPHATE; BACTERIAL ENZYME; ISOMERASE; ISOTOPE; PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BINDING KINETICS; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; MOLECULAR MODEL; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PH; PRIORITY JOURNAL; STEADY STATE; TEMPERATURE;

ALDOSE-KETOSE ISOMERASES; KINETICS; MOLECULAR STRUCTURE; MULTIENZYME COMPLEXES; MYCOBACTERIUM TUBERCULOSIS; OXIDOREDUCTASES; SIGNAL TRANSDUCTION;

ESCHERICHIA COLI; MYCOBACTERIUM TUBERCULOSIS;

EID: 84863521755     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300513r     Document Type: Article

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