Gene cloning, expression, and biochemical characterization of an alkali-tolerant β-mannanase from Humicola insolens Y1

Journal of Industrial Microbiology and Biotechnology

Volumn 39, Issue 4, 2012, Pages 547-555

  Luo, Huiying ^a   Wang, Kun ^a   Huang, Huoqing ^a   Shi, Pengjun ^a   Yang, Peilong ^a   Yao, Bin ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Mannanase; Alkali tolerance; Heterologous expression; Humicola insolens; Pichia pastoris

Indexed keywords

ARGININE; BETA MANNOSIDASE; COMPLEMENTARY DNA; FUNGAL DNA; FUNGAL ENZYME; LYSINE;

ALKALINITY; ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME STABILITY; FUNGUS; GENE EXPRESSION; HUMICOLA INSOLENS Y1; HYDROLYSIS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PICHIA PASTORIS; PROTEIN PURIFICATION;

ASCOMYCOTA; BETA-MANNOSIDASE; CLONING, MOLECULAR; HYDROGEN-ION CONCENTRATION; INDUSTRIAL MICROBIOLOGY; PICHIA; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS;

HUMICOLA INSOLENS; PICHIA PASTORIS;

EID: 84863454676     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-011-1067-8     Document Type: Article

References (38)

1. Altschul SF, Madden T, SchaVer A, Zhang J, Zhang Z, Miller W, Lipman D (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402 (Pubitemid 27359211)
2. Bendtsen JD, Nielsen H, Heijne G, Brunak S (2004) Improved prediction of signal peptides: signalP 3.0. J Mol Biol 340:783-795 (Pubitemid 38829638)
3. Benech RO, Li XM, Patton D, Powlowski J, Storms R, Bourbonnais R, Paice M, Tsang A (2007) Recombinant expression, characterization, and pulp prebleaching property of a Phanerochaete chrysosporium endo-α-1,4- mannanase. Enzyme Microbiol Technol 41:740-747 (Pubitemid 47361324)
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
5. DayhoV MO, Schwartz RM, Orcutt BC (1978) A model of evolutionary change in proteins. In: DayhoV MO (ed) Atlas of protein sequence and structure, vol 5, suppl 3. National Biomedical Research Foundation, Washington, pp 345-352
6. Dhawan S, Kaur J (2007) Microbial mannanases: an overview of production and applications. Crit Rev Biotechnol 27:197-216 (Pubitemid 351419000)
7. Filho EXF (1998) Hemicellulases and biotechnology. In: Pandalai SG (ed) Recent research developments in microbiology. Research Signpost, Trivandrum, pp 165-176
8. Granchi L, Bosco M, Vicenzini M (1999) Rapid detection and quantiWcation of yeast species during spontaneous wine fermentation by PCR-RFLP analysis of the rDNA ITS region. Appl Microbiol 87:949-956 (Pubitemid 30055710)
9. Henrissat B, Bairoch A (1993) New families in the classiWcation of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293:781-788 (Pubitemid 23244564)
10. Hilge M, Gloor SM, Rypniewski W, Sauer O, Heightman TD, Zimmermann W, Winterhalter K, Piontek K (1998) High-resolution native and complex structures of thermostableα-mannanase from Thermomonospora fusca-substrate speciWcity in glycosyl hydrolase family 5. Structure 6:1433-1444 (Pubitemid 28541886)
11. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
12. Le Nours J, Ryttersgaard C, Lo Leggio L, østergaard PR, Borchert TV, Christensen LL, Larsen S (2003) Structure of two fungalα-1,4- galactanases: searching for the basis for temperature and pH optimum. Protein Sci 12:1195-1204 (Pubitemid 36597506)
13. Liepman AH, Nairn CJ, Willats WGT, Sørensen I, Roberts AW, Keegstra K (2007) Functional genomic analysis supports conservation of function among cellulose synthase-like A gene family members and suggest diverse roles of mannans in plants. Plant Physiol 143:1881-1893 (Pubitemid 46849495)
14. Liu YG, Whittier RF (1995) Thermal asymmetric interlaced PCR: automatable ampliWcation and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25:674-681
15. Luo H, Wang Y, Wang H, Yang J, Yang Y, Huang H, Yang P, Bai Y, Shi P, Fan Y, Yao B (2009) A novel highly acidicα-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris. Appl Microbiol Biotechnol 82:453-461
16. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426-428
17. Moreira LRS, Filho EXF (2008) An overview of mannan structure and mannan-degrading enzyme systems. Appl Microbiol Biotechnol 79:165-178
18. Nicholas KB, Nicholas HB Jr. (1997) GeneDoc: a tool for editing and annotating multiple sequence alignments. (Distributed by the author. http://www.psc.edu/biomed/genedoc)
19. Nielsen JE, McCammon JA (2003) Calculating pKa values in enzyme active sites. Protein Sci 12:1894-1901 (Pubitemid 37022812)
20. Ootsuka S, Saga N, Suzuki KI, Inoue A, Ojima T (2006) Isolation and cloning of an endo-α-1,4-mannanase from PaciWc abalone Haliotis discus hannai. J Biotechnol 125:269-280 (Pubitemid 44188950)
21. Petkowicz CLO, Reicher F, Chanzy H, Taravel FR, Vuong R (2001) Linear mannan in the endosperm of Schizolobium amazonicum. Carbohydr Polym 44:107-112 (Pubitemid 32030088)
22. Regalado C, Garcia-Almendarez BE, Venegas-Barrera LM, Tellez-Jurado A, Rodriguez-Serrano G, Huerta-Ochoa S, Whitaker JR (2000) Production, partial puriWcation and properties ofα-mannanases obtained by solid substrate fermentation of spent soluble coVee wastes and copra paste using Aspergillus oryzae and Aspergillus niger. J Sci Food Agric 80:1343-1350 (Pubitemid 30411337)
23. Sabini E, Schubert H, Murshudov G, Wilson KS, Siika-Aho M, Penttilä M (2000) The three-dimensional structure of a Trichoderma reeseiα- mannanase from glycoside hydrolase family 5. Acta Crystallogr D Biol Crystallogr 56:3-13 (Pubitemid 30061536)
24. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
25. Setati ME, Ademark P, van Zyl WH, Hahn-Hägerdal B, Stålbrand H (2001) Expression of the Aspergillus aculeatus endo-α-1,4-mannanase encoding gene (man1) in Saccharomyces cerevisiae and characterization of the recombinant enzyme. Protein Expr Purif 21:105-114 (Pubitemid 32620566)
26. Shi P, Yuan T, Zhao J, Huang H, Luo H, Meng K, Wang Y, Yao B (2011) Genetic and biochemical characterization of a proteaseresistant mesophilicα-mannanase from Streptomyces sp. S27. J Ind Microbiol Biotechnol 38:451-458
27. Shimahara H, Suzuki H, Sugiyama N, Nisizawa K (1975) Partial puriWcation ofα-mannanase from the konjac tubers and their substrate speciWcity in relation to the structure of konjac glucomannan. Agric Biol Chem 39:301-312
28. Stålbrand H, Siika-Aho M, Tenkanen M, Viikari L (1993) PuriWcation and characterisation of twoα-mannanases from Trichoderma reesei. J Biotechnol 29:229-242 (Pubitemid 23178104)
29. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-1599 (Pubitemid 47236692)
30. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-speciWc gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680 (Pubitemid 24354800)
31. Tynan-Connolly BM, Nielsen JE (2007) Redesigning protein pKa values. Protein Sci 16:239-249
32. van Zyl WH, Rosea SH, Trollopeb K, Görgensb JF (2010) Fungalα-mannanases: mannan hydrolysis, heterologous production and biotechnological applications. Process Biochem 45:1203-1213
33. Wong KKY, Saddler JN (1993) Applications of hemicellulases in the food, feed and pulp and paper industries. In: Coughlan MP, Hazlewood PG (eds) Hemicellulose and hemicellulases. Portland Press, London, pp 127-143
34. Wu G, Bryant MM, Voitle RA, Roland DA (2005) EVects ofα-mannanase in corn-soy diets on commercial leghorns in secondcycle hens. Poultry Sci 84:894-897 (Pubitemid 40793727)
35. Ximenes EA, Chen H, Kataeva IA, Cotta MA, Felix CR, Ljungdahl LG, Li XL (2005) A mannanase, ManA, of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 has carbohydrate binding and docking modules. Can J Microbiol 51:559-568 (Pubitemid 41722112)
36. Yang P, Shi P, Wang Y, Bai Y, Meng K, Luo H, Yuan T, Yao B (2007) Cloning and overexpression of a Paenibacillusα-glucanase in Pichia pastoris: puriWcation and characterization of the recombinant enzyme. J Microbiol Biotechnol 17:58-66 (Pubitemid 46218106)
37. Zhao J, Shi P, Luo H, Yang P, Zhao H, Bai Y, Huang H, Wang H, Yao B (2010) An acidophilic and acid-stableα-mannanase from Phialophora sp. p13 with high mannan hydrolysis activity under simulated gastric conditions. J Agric Food Chem 58:3184-3190
38. Zhao Y, Zhang Y, Cao Y, Qi J, Mao L, Xue Y, Gao F, Peng H, Wang X, Gao GF, Ma Y (2011) Structural analysis of alkalineα-mannanase from alkaliphilic Bacillus sp. N16-5: implications for adaptation to alkaline conditions. PLoS One 6:1-12