메뉴 건너뛰기




Volumn 44, Issue 7, 2012, Pages 614-622

Analysis of reference gene expression for real-time PCR based on relative quantitation and dual spike-in strategy in the silkworm Bombyx mori

Author keywords

dual spike in strategy; normalization; real time quantitative PCR; reference gene; silkworm Bombyx mori

Indexed keywords

ACTIN; ECDYSTERONE; GLUTATHIONE TRANSFERASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INSECT PROTEIN; RIBOSOME PROTEIN; RIBOSOME PROTEIN L3; RNA 28S; RUTOSIDE; TATA BINDING PROTEIN; TUBULIN; UBIQUITIN C;

EID: 84863450247     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1093/abbs/gms040     Document Type: Article
Times cited : (25)

References (20)
  • 2
    • 77349119216 scopus 로고    scopus 로고
    • Identification of endogenous control genes for normalisation of real-time quantitative PCR data in colorectal cancer
    • Kheirelseid EA, Chang KH, Newell J, Kerin MJ and Miller N. Identification of endogenous control genes for normalisation of real-time quantitative PCR data in colorectal cancer. BMC Mol Biol 2010, 11: 12-25.
    • (2010) BMC Mol. Biol. , vol.11 , pp. 12-25
    • Kheirelseid, E.A.1    Chang, K.H.2    Newell, J.3    Kerin, M.J.4    Miller, N.5
  • 3
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • 3R: 0034.1-0034.11
    • Vandesompele J, Preter KD, Pattyn F, Poppe B, Van Roy N, De Paepe A and Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3R: 0034.1-0034.11.
    • (2002) Genome. Biol.
    • Vandesompele, J.1    Preter, K.D.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 5
    • 0036668567 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems
    • Bustin SA. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. J Mol Endocrinol 2002, 29: 23-39.
    • (2002) J. Mol. Endocrinol. , vol.29 , pp. 23-39
    • Bustin, S.A.1
  • 6
    • 27144456802 scopus 로고    scopus 로고
    • Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress
    • Nicot N, Hausman JF, Hoffmann L and Evers D. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 2005, 56: 2907-2914.
    • (2005) J. Exp. Bot. , vol.56 , pp. 2907-2914
    • Nicot, N.1    Hausman, J.F.2    Hoffmann, L.3    Evers, D.4
  • 7
    • 67349112357 scopus 로고    scopus 로고
    • A decade of improvements in quantification of gene expression and internal standard selection
    • Thellin O, ElMoualij B, Heinen E and Zorzi W. A decade of improvements in quantification of gene expression and internal standard selection. Biotechnol Adv 2009, 27: 323-333.
    • (2009) Biotechnol. Adv. , vol.27 , pp. 323-333
    • Thellin, O.1    ElMoualij, B.2    Heinen, E.3    Zorzi, W.4
  • 9
    • 0034694128 scopus 로고    scopus 로고
    • Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR
    • Schmittgen TD and Zakrajsek BA. Effect of experimental treatment on housekeeping gene expression: Validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods 2000, 46: 69-81.
    • (2000) J. Biochem. Biophys. Methods , vol.46 , pp. 69-81
    • Schmittgen, T.D.1    Zakrajsek, B.A.2
  • 11
    • 33745610748 scopus 로고    scopus 로고
    • Selection of a set of reliable reference genes for quantitative real-time PCR in normal equine skin and in equine sarcoids
    • Bogaert L, Van Poucke M, De Baere C, Peelman L, Gasthuys F and Martens A. Selection of a set of reliable reference genes for quantitative real-time PCR in normal equine skin and in equine sarcoids. BMC Biotechnol 2006, 6: 24-31.
    • (2006) BMC Biotechnol. , vol.6 , pp. 24-31
    • Bogaert, L.1    Van Poucke, M.2    De Baere, C.3    Peelman, L.4    Gasthuys, F.5    Martens, A.6
  • 12
    • 77950883616 scopus 로고    scopus 로고
    • Validation of endogenous reference genes for insecticide-induced and developmental expression profiling of Liposcelis bostsrychophila (Psocoptera: Liposcelididae)
    • Jiang HB, Liu YH, Tang PA, Zhou AW and Wang JJ. Validation of endogenous reference genes for insecticide-induced and developmental expression profiling of Liposcelis bostsrychophila (Psocoptera: Liposcelididae). Mol Biol Rep 2010, 37: 1019-1029.
    • (2010) Mol. Biol. Rep. , vol.37 , pp. 1019-1029
    • Jiang, H.B.1    Liu, Y.H.2    Tang, P.A.3    Zhou, A.W.4    Wang, J.J.5
  • 13
    • 79957856248 scopus 로고    scopus 로고
    • Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster
    • Ponton F, Chapuis MP, Pernice M, Sword GA and Simpson SJ. Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster. J Insect Physiol 2011, 57: 840-850.
    • (2011) J. Insect. Physiol. , vol.57 , pp. 840-850
    • Ponton, F.1    Chapuis, M.P.2    Pernice, M.3    Sword, G.A.4    Simpson, S.J.5
  • 14
    • 64149097786 scopus 로고    scopus 로고
    • The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments
    • Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M and Mueller R, et al. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009, 55: 611-622.
    • (2009) Clin. Chem. , vol.55 , pp. 611-622
    • Bustin, S.A.1    Benes, V.2    Garson, J.A.3    Hellemans, J.4    Huggett, J.5    Kubista, M.6    Mueller, R.7
  • 15
    • 69949096465 scopus 로고    scopus 로고
    • Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy
    • Zhang Y, Wei ZG, Li YY, Chen Y, Shen W and Lu C. Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy. Anal Biochem 2009, 394: 202-208.
    • (2009) Anal. Biochem. , vol.394 , pp. 202-208
    • Zhang, Y.1    Wei, Z.G.2    Li, Y.Y.3    Chen, Y.4    Shen, W.5    Lu, C.6
  • 16
    • 0141594606 scopus 로고    scopus 로고
    • Not just for housekeeping: Protein initiation and elongation factors in cell growth and tumorigenesis
    • Thornton S, Anand N, Purcell D and Lee J. Not just for housekeeping: Protein initiation and elongation factors in cell growth and tumorigenesis. J Mol Med 2003, 81: 536-548.
    • (2003) J. Mol. Med. , vol.81 , pp. 536-548
    • Thornton, S.1    Anand, N.2    Purcell, D.3    Lee, J.4
  • 17
    • 0037227141 scopus 로고    scopus 로고
    • Exogenous reference RNA for normalization of real-time quantitative PCR
    • Smith RD, Brown B, Ikonomi P and Schechter AN. Exogenous reference RNA for normalization of real-time quantitative PCR. Biotechnol Tech 2003, 34: 88-91.
    • (2003) Biotechnol. Tech. , vol.34 , pp. 88-91
    • Smith, R.D.1    Brown, B.2    Ikonomi, P.3    Schechter, A.N.4
  • 18
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using realtime quantitative PCR and the 22DDCT method
    • Livak KJ and Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 22DDCT method. Methods 2001, 25: 402-408.
    • (2001) Methods , vol.25 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2
  • 20
    • 34247645532 scopus 로고    scopus 로고
    • Universal reference method for real-time PCR gene expression analysis of preimplantation embryos
    • Bower NI, Moser RJ, Hill JR and Lehnert SA. Universal reference method for real-time PCR gene expression analysis of preimplantation embryos. Biotechniques 2007, 42: 199-206.
    • (2007) Biotechniques , vol.42 , pp. 199-206
    • Bower, N.I.1    Moser, R.J.2    Hill, J.R.3    Lehnert, S.A.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.