A faster immunofluorescence assay for tracking infection progress of human cytomegalovirus

Acta Biochimica et Biophysica Sinica

Volumn 44, Issue 7, 2012, Pages 597-605

  Duan, Yingliang ^a   Miao, Lingfeng ^a   Ye, Hanqing ^a   Yang, Cuiqing ^a   Fu, Bishi ^a   Schwartz, Philip H ^b   Rayner, Simon ^a   Fortunato, Elizabeth A ^c   Luo, Min Hua ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b CHILDREN S HOSPITAL OF ORANGE COUNTY   (United States)

^c UNIVERSITY OF IDAHO   (United States)

Author keywords

human cytomegalovirus; immunofluorescence assay; viral gene expression; virus entry; virus replication

Indexed keywords

CYTOMEGALOVIRUS MATRIX PROTEIN 65KDA; DNA BINDING PROTEIN; ICP36 PROTEIN, CYTOMEGALOVIRUS; IE1 PROTEIN, CYTOMEGALOVIRUS; IMMEDIATE EARLY PROTEIN; MATRIX PROTEIN; PHOSPHOPROTEIN; VIRUS PROTEIN;

ARTICLE; CELL CULTURE; COST BENEFIT ANALYSIS; CYTOLOGY; CYTOMEGALOVIRUS; ECONOMICS; FIBROBLAST; FLUORESCENCE MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; GLIOBLASTOMA; GROWTH, DEVELOPMENT AND AGING; HUMAN; LUNG; METABOLISM; METHODOLOGY; NEURAL STEM CELL; PATHOLOGY; PRENATAL DEVELOPMENT; REPRODUCIBILITY; TIME; TUMOR CELL LINE; VIROLOGY; VIRUS ENTRY;

CELL LINE, TUMOR; CELLS, CULTURED; COST-BENEFIT ANALYSIS; CYTOMEGALOVIRUS; DNA-BINDING PROTEINS; FIBROBLASTS; FLUORESCENT ANTIBODY TECHNIQUE; GLIOBLASTOMA; HUMANS; IMMEDIATE-EARLY PROTEINS; LUNG; MICROSCOPY, FLUORESCENCE; NEURAL STEM CELLS; PHOSPHOPROTEINS; REPRODUCIBILITY OF RESULTS; TIME FACTORS; VIRAL MATRIX PROTEINS; VIRAL PROTEINS; VIRUS INTERNALIZATION;

HUMAN HERPESVIRUS 5;

EID: 84863437440     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1093/abbs/gms041     Document Type: Article

References (27)

1. Didier ES, Orenstein JM, Aldras A, Bertucci D, Rogers LB and Janney FA. Comparison of three staining methods for detecting microsporidia in fluids. J Clin Microbiol 1995, 33: 3138-3145.
2. Taber LH, Brasier F, Couch RB, Greenberg SB, Jones D and Knight V. Diagnosis of herpes simplex virus infection by immunofluorescence. J Clin Microbiol 1976, 3: 309-312.
3. Lee AW, Hertel L, Louie RK, Burster T, Lacaille V, Pashine A and Abate DA, et al. Human cytomegalovirus alters localization of MHC class II and dendrite morphology in mature Langerhans cells. J Immunol 2006, 177: 3960-3971.
4. Rogers SL and Rogers GC. Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy. Nat Protoc 2008, 3: 606-611.
5. Strang BL, Boulant S and Coen DM. Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication. J Virol 2010, 84: 1771-1784.
6. Luo MH, Schwartz PH and Fortunato EA. Neonatal neural progenitor cells and their neuronal and glial cell derivatives are fully permissive for human cytomegalovirus infection. J Virol 2008, 82: 9994-10007.
7. Luo MH, Rosenke K, Czornak K and Fortunato EA. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol 2007, 81: 1934-1950.
8. Luo MH and Fortunato EA. Long-term infection and shedding of human cytomegalovirus in T98G glioblastoma cells. J Virol 2007, 81: 10424-10436.
9. Chen LY, Dai G, Wu GJ, Wang JW, Luo MH and Chen SZ. Influence of human cytomegalovirus infection on the expression of HOX genes in human embryo lung cells. Hunan Yi Ke Da Xue Xue Bao 2000, 25: 440-442.
10. Luo MH, Hannemann H, Kulkarni AS, Schwartz PH, O'Dowd JM and Fortunato EA. Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells. J Virol 2010, 84: 3528-3541.
11. Britt WJ and Vugler L. Structural and immunological characterization of the intracellular forms of an abundant 68,000 Mr human cytomegalovirus protein. J Gen Virol 1987, 68 (Pt 7): 1897-1907.
12. Casavant NC, Luo MH, Rosenke K, Winegardner T, Zurawska A and Fortunato EA. Potential role for p53 in the permissive life cycle of human cytomegalovirus. J Virol 2006, 80: 8390-8401.
13. Fortunato EA and Spector DH. p53 and RPA are sequestered in viral replication centers in the nuclei of cells infected with human cytomegalovirus. J Virol 1998, 72: 2033-2039.
14. Rosenke K and Fortunato EA. Bromodeoxyuridine-labeled viral particles as a tool for visualization of the immediate-early events of human cytomegalovirus infection. J Virol 2004, 78: 7818-7822.
15. Iwayama S, Yamamoto T, Furuya T, Kobayashi R, Ikuta K and Hirai K. Intracellular localization and DNA-binding activity of a class of viral early phosphoproteins in human fibroblasts infected with human cytomegalovirus (Towne strain). J Gen Virol 1994, 75 (Pt 12): 3309-3318.
16. Mocarski ES, Pereira L and Michael N. Precise localization of genes on large animal virus genomes: Use of lambda gt11 and monoclonal antibodies to map the gene for a cytomegalovirus protein family. Proc Natl Acad Sci USA 1985, 82: 1266-1270.
17. Penfold ME and Mocarski ES. Formation of cytomegalovirus DNA replication compartments defined by localization of viral proteins and DNA synthesis. Virology 1997, 239: 46-61.
18. Jault FM, Spector SA and Spector DH. The effects of cytomegalovirus on human immunodeficiency virus replication in brain-derived cells correlate with permissiveness of the cells for each virus. J Virol 1994, 68: 959-973.
19. Cheeran MC, Hu S, Ni HT, Sheng W, Palmquist JM, Peterson PK and Lokensgard JR. Neural precursor cell susceptibility to human cytomegalovirus diverges along glial or neuronal differentiation pathways. J Neurosci Res 2005, 82: 839-850.
20. Melnick M, Mocarski ES, Abichaker G, Huang J and Jaskoll T. Cytomegalovirus-induced embryopathology: Mouse submandibular salivary gland epithelial-mesenchymal ontogeny as a model. BMC Dev Biol 2006, 6: 42.
21. Shinmura Y, Kosugi I, Kaneta M and Tsutsui Y. Migration of virus-infected neuronal cells in cerebral slice cultures of developing mouse brains after in vitro infection with murine cytomegalovirus. Acta Neuropathol 1999, 98: 590-596.
22. Odeberg J, Wolmer N, Falci S, Westgren M, Sundtrom E, Seiger A and Soderberg-Naucler C. Late human cytomegalovirus (HCMV) proteins inhibit differentiation of human neural precursor cells into astrocytes. J Neurosci Res 2007, 85: 583-593.
23. Shinmura Y, Aiba-Masago S, Kosugi I, Li RY, Baba S and Tsutsui Y. Differential expression of the immediate-early and early antigens in neuronal and glial cells of developing mouse brains infected with murine cytomegalovirus. Am J Pathol 1997, 151: 1331-1340.
24. Ranneberg-Nilsen T, Dale HA, Luna L, Slettebakk R, Sundheim O, Rollag H and Bjoras M. Characterization of human cytomegalovirus uracil DNA glycosylase (UL114) and its interaction with polymerase processivity factor (UL44). J Mol Biol 2008, 381: 276-288.
25. Bhattacharyya D, Hammond AT and Glick BS. High-quality immunofluorescence of cultured cells. Methods Mol Biol 2010, 619: 403-410.
26. Schneider Gasser EM, Straub CJ, Panzanelli P, Weinmann O, Sassoe-Pognetto M and Fritschy JM. Immunofluorescence in brain sections: Simultaneous detection of presynaptic and postsynaptic proteins in identified neurons. Nat Protoc 2006, 1: 1887-1897.
27. Boppana SB, Ross SA, Shimamura M, Palmer AL, Ahmed A, Michaels MG and Sanchez PJ, et al. Saliva polymerase-chain-reaction assay for cytomegalovirus screening in newborns. N Engl J Med 2011, 364: 2111-2118.