LC-MS analysis of polyclonal human anti-Neu5Gc xeno-autoantibodies immunoglobulin G subclass and partial sequence using multistep intravenous immunoglobulin affinity purification and multienzymatic digestion

Analytical Chemistry

Volumn 84, Issue 6, 2012, Pages 2761-2768

  Lu, Qiaozhen ^a   Padler Karavani, Vered ^b   Yu, Hai ^c   Chen, Xi ^c   Wu, Shiaw Lin ^a   Varki, Ajit ^b   Hancock, William S ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

^b University of California San Diego   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY PURIFICATION; BINDING PATTERN; CHRONIC INFLAMMATION; DIGESTION PROCEDURE; IMMUNE RESPONSE; IMMUNOGLOBULIN G; LC-MS ANALYSIS; MULTI-STEP; N-GLYCOLYLNEURAMINIC ACID; NANO LIQUID CHROMATOGRAPHY; NANO-LC; PARTIAL SEQUENCES; PEPTIDE SEQUENCES; POLYCLONAL; POLYCLONAL IGG; SIALIC ACIDS;

ANTIGENS; CARBOHYDRATES; CARBOXYLIC ACIDS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PURIFICATION;

ANTIBODIES;

AUTOANTIBODY; IMMUNOGLOBULIN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G; N GLYCOLOYLNEURAMINIC ACID; N-GLYCOLYLNEURAMINIC ACID; NEURAMINIC ACID DERIVATIVE; TUMOR MARKER;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; HUMAN; IMMUNOLOGY; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR GENETICS; NEOPLASM; PAN TROGLODYTES; PROTEIN MICROARRAY;

AMINO ACID SEQUENCE; ANIMALS; AUTOANTIBODIES; CHROMATOGRAPHY, AFFINITY; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; IMMUNOGLOBULINS, INTRAVENOUS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NEOPLASMS; NEURAMINIC ACIDS; PAN TROGLODYTES; PROTEIN ARRAY ANALYSIS; TUMOR MARKERS, BIOLOGICAL;

EID: 84863344855     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac2030893     Document Type: Article

References (27)

1. Nguyen, D. H.; Tangvoranuntakul, P.; Varki, A. J. Immunol. 2005, 175, 228-236
2. Kelm, S.; Schauer, R. Int. Rev. Cytol. 1997, 175, 137-240
3. Traving, C.; Schauer, R. Cell. Mol. Life Sci. 1998, 54, 1330-1349
4. Varki, A. Glycobiology 1992, 2, 25-40
5. Angata, T.; Varki, A. Chem. Rev. 2002, 102, 439-469
6. Chou, H. H.; Takematsu, H.; Diaz, S.; Iber, J.; Nickerson, E.; Wright, K. L.; Muchmore, E. A.; Nelson, D. L.; Warren, S. T.; Varki, A. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 11751-11756
7. Muchmore, E. A.; Diaz, S.; Varki, A. Am. J. Phys. Anthropol. 1998, 107, 187-198
8. Tangvoranuntakul, P.; Gagneux, P.; Diaz, S.; Bardor, M.; Varki, N.; Varki, A.; Muchmore, E. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 12045-12050
9. Higashi, H.; Nishi, Y.; Fukui, Y.; Ikuta, K.; Ueda, S.; Kato, S.; Fujita, M.; Nakano, Y.; Taguchi, T.; Sakai, S. Gann 1984, 75, 1025-1029
10. Hirabayashi, Y.; Higashi, H.; Kato, S.; Taniguchi, M.; Matsumoto, M. Jpn. J. Cancer Res. (Gann) 1987, 78, 614-620
11. Nakarai, H.; Saida, T.; Shibata, Y.; Irie, R. F.; Kano, K. Int. Arch. Allergy Appl. Immunol. 1987, 83, 160-166
12. Ohashi, Y.; Sasabe, T.; Nishida, T.; Nishi, Y.; Higashi, H. Am. J. Ophthalmol. 1983, 96, 321-325
13. Marquina, G.; Waki, H.; Fernandez, L. E.; Kon, K.; Carr, A.; Valiente, O.; Perez, R.; Ando, S. Cancer Res. 1996, 56, 5165-5171
14. Kawai, T.; Kato, A.; Higashi, H.; Kato, S.; Naiki, M. Cancer Res. 1991, 51, 1242-1246
15. Devine, P. L.; Clark, B. A.; Birrell, G. W.; Layton, G. T.; Ward, B. G.; Alewood, P. F.; McKenzie, I. F. C. Cancer Res. 1991, 51, 5826-5836
16. Zhu, A.; Hurst, R. Xenotransplantation 2002, 9, 376-381
17. Padler-Karavani, V.; Yu, H.; Cao, H.; Chokhawala, H.; Karp, F.; Varki, N.; Chen, X.; Varki, A. Glycobiology 2008, 18, 818-830
18. Hedlund, M.; Padler-Karavani, V.; Varki, N.; Varki, A. Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 18936-18941
19. Padler-Karavani, V.; Hurtado-Ziola, N.; Pu, M.; Yu, H.; Huang, S.; Muthana, S.; Chokhawala, H.; Cao, H.; Secrest, P.; Friedmann-Morvinski, D.; Singer, O.; Ghaderi, D.; Verma, I. M.; Liu, Y.-T.; Messer, K.; Chen, X.; Varki, A.; Schwab, R. Cancer Res. 2011, 71, 3352-3363
20. de Costa, D.; Broodman, I.; Vanduijn, M. M.; Stingl, C.; Dekker, L. J.; Burgers, P. C.; Hoogsteden, H. C.; Sillevis Smitt, P. A.; van Klaveren, R. J.; Luider, T. M. J. Proteome Res. 2010, 9, 2937-2945
21. Jiang, H.; Wu, S.-L.; Karger, B. L.; Hancock, W. S. Anal. Chem. 2010, 82, 6154-6162
22. Hober, S.; Nord, K.; Linhult, M. J. Chromatogr., B: Anal. Technol. Biomed. Life Sci. 2007, 848, 40-47
23. Andrew, S. M.; Titus, J. A. Curr. Protoc. Immunol. 2001, Chapter 2, Unit 2.7.
24. Jacobs, D. S.; De Mott, W. R.; Oxley, D. K., Eds.; Jacobs & DeMott Laboratory Test Handbook, 5 th ed.; Lexi-Comp Inc.: Hudson, OH, 2001; p 536.
25. Barrett, D. J.; Ayoub, E. M. Clin. Exp. Immunol. 1986, 63, 127-134
26. Hammarström, L.; Persson, M. A.; Smith, C. I. Immunology 1985, 54, 821-826
27. von Gunten, S.; Smith, D. F.; Cummings, R. D.; Riedel, S.; Miescher, S.; Schaub, A.; Hamilton, R. G.; Bochner, B. S. J. Allergy Clin. Immunol. 2009, 123, 1268-1276