Intense picosecond pulsed electric fields induce apoptosis through a mitochondrial-mediated pathway in HeLa cells

Molecular Medicine Reports

Volumn 5, Issue 4, 2012, Pages 981-987

  Hua, Yuan Yuan ^a   Wang, Xiao Shu ^a   Zhang, Yu ^a   Yao, Chen Guo ^b   Zhang, Xi Ming ^b   Xiong, Zheng Ai ^a  

^a CHONGQING MEDICAL UNIVERSITY   (China)

^b CHONGQING UNIVERSITY   (China)

Author keywords

Apoptosis; HeLa cells; Intense picosecond pulsed electric fields; Mitochondrial pathway

Indexed keywords

APOPTOSIS INDUCING FACTOR; CASPASE 3; CASPASE 9; CYTOCHROME C; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53; RHODAMINE 123;

APOPTOSIS; ARTICLE; CELL PROLIFERATION; CELL ULTRASTRUCTURE; CONCENTRATION RESPONSE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOSOL; DOWN REGULATION; ENZYME ACTIVATION; GROWTH INHIBITION; HELA CELL; HUMAN; HUMAN CELL; MEMBRANE DEPOLARIZATION; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; PICOSECOND PULSED ELECTRIC FIELD; PULSED ELECTRIC FIELD; TRANSMISSION ELECTRON MICROSCOPY; UPREGULATION;

APOPTOSIS; APOPTOSIS INDUCING FACTOR; BCL-2-ASSOCIATED X PROTEIN; CASPASE 3; CASPASE 9; CYTOCHROMES C; ELECTRICITY; HELA CELLS; HUMANS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MICROSCOPY, CONFOCAL; MITOCHONDRIA; PROTO-ONCOGENE PROTEINS C-BCL-2; RHODAMINE 123; TIME FACTORS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84863269081     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2012.780     Document Type: Article

References (26)

1. Weaver JC: Electroporation: a general phenomenon for manipulating cells and tissues. J Cell Biochem 51: 426-435, 1993.
2. Okino M, Tomie H, Kanesada H, et al: Optimal electric conditions in electrical impulse chemotherapy. Jpn J Cancer Res 83: 1095-1101, 1992.
3. Hofmann GA, Dev SB, Dimmer S, et al: Electroporation therapy: a new approach for the treatment of head and neck cancer. IEEE Trans Biomed Eng 46: 752-759, 1999. (Pubitemid 29245066)
4. Dev SB, Rabussay DP, Widera G, et al: Medical applications of electroporation. IEEE Trans Plasma Sci 28: 206-223, 2000.
5. Tien HT and Ottova A: The bilayer lipid membrane (BLM) under electrical field. IEEE Trans Dielectr Electr Insul 10: 717-727, 2003.
6. Miller L, Leor J and Rubinsky B: Cancer cell ablation with irreversible electroporation. Technol Cancer Res Treat 4: 699-705, 2005. (Pubitemid 43083355)
7. Rubinsky J, Onik G, Mikus P and Rubinsky B: Optimal parameters for the destruction of prostate cancer using irreversible electroporation. J Urol 180: 2668-2674, 2008.
8. Stacey M, Stickley J, Fox P, Statler V, Schoenbach K, Beebe SJ and Buescher S: Differential effects in cells exposed to ultra-short, high intensity electric fields: cell survival, DNA damage, and cell cycle analysis. Mutat Res 542: 65-75, 2003. (Pubitemid 37486099)
9. Katsuki S, Nomura N, Koga H, et al: Biological effects of narrow band pulsed electric fields. IEEE Trans Dielectr Electr Insul 14: 663-668, 2007. (Pubitemid 46906846)
10. Chen N, Garner AL, Chen G, et al: Nanosecond electric pulses penetrate the nucleus and enhance speckle formation. Biochem Biophys Res Commun 364: 220-225, 2007. (Pubitemid 47633404)
11. Craviso GL, Chatterjee P, Maalouf G, et al: Nanosecond electric pulse-induced increase in intracellular calcium in adrenal chromaffin cells triggers calcium-dependent catecholamine release. IEEE Trans Dielectr Electr Insul 16: 1294-1301, 2009.
12. Joshi RP and Schoenbach KH: Bioelectric effects of intense ultrashort pulses. Crit Rev Biomed Eng 38: 255-304, 2010.
13. Baldwin WH, Gregory BW, Osgood CJ, et al: Membrane permeability and cell survival after nanosecond pulsed electric field exposure-significance of exposure media composition. IEEE Trans Plasma Sci 38: 2948-2953, 2010.
14. Stacey M, Fox P, Buescher S and Kolb J: Nanosecond pulsed electric field induced cytoskeleton, nuclear membrane and telomere damage adversely impact cell survival. Bioelectrochemistry 82: 131-134, 2011.
15. Chen X, Chen X, Swanson RJ, Schoenbach KH, Yin S and Zheng S: Histopathological follow-up by tissue micro-array in a survival study after melanoma treated by nanosecond pulsed electric fields (nsPEF). J Dermatolog Treat 22: 153-161, 2011.
16. Baum CE, Stone AP and Tyo JS (eds): Ultra-Wideband, Short-Pulse Electromagnetics 8. Springer Press, New York, NY, 2007.
17. Bajracharya C, Shu X, Baum CE and Schoenbach KH: Target detection with impulse radiating antenna. IEEE Antennas Wireless Propag Lett 10: 496-499, 2011.
18. Bray F, Loos AH, McCarron P, Weiderpass E, Arbyn M, Møller H, Hakama M and Parkin DM: Trends in cervical squamous cell carcinoma incidence in 13 European countries: changing risk and the effects of screening. Cancer Epidemiol Biomarkers Prev 14: 677-686, 2005. (Pubitemid 40489214)
19. Karimi Zarchi M, Mousavi A, Malekzadeh M, Dehghani A, Behnamfar Z and Godarzi A: Conservative treatment in young patients with cervical cancer: a review. Asian Pac J Cancer Prev 11: 589-594, 2010.
20. Yao C, Mo D, Li C, Sun C and Mi Y: Study of transmembrane potentials of inner and outer membranes induced by pulsed-electric-field model and simulation. IEEE Trans Plasma Sci 35: 1541-1549, 2007. (Pubitemid 47603944)
21. Yao C, Mi Y, Li C, et al: Study of transmembrane potentials on cellular inner and outer membrane-frequency response model and its filter characteristic simulation. IEEE Trans Biomed Eng 55: 1792-1799, 2008. (Pubitemid 351871648)
22. Schoenbach KH, Katsuki S, Akiyama H, et al: Biological effects of intense subnanosecond electrical pulses. Power Modulator Symposium, 2006. Conference Record of the 2006 Twenty-Seventh International: May 14-18, 573-576, 2006.
23. Ermolina I, Polevaya YL, Feldman YR, et al: Study of normal and malignant white blood cells by time domain dielectric spectroscopy. IEEE Trans Dielectr Electr Insul 8: 253-261, 2001. (Pubitemid 32444407)
24. Feldman YR, Ermolina I and Hayashi Y: Time domain dielectric spectroscopy study of biological systems. IEEE Trans Dielectr Electr Insul 10: 728-753, 2003.
25. Schoenbach KH, Shu X, Joshi RP, Camp JT, Heeren T, Kolb JF and Beebe SJ: The effect of intense subnanosecond electrical pulses on biological cells. IEEE Trans Plasma Sci 36: 414-422, 2008. (Pubitemid 351597102)
26. Camp JT, Shu X, Beebe SJ, Blackmore PF and Schoenbach KH: Bioelectric studies with subnanosecond pulsed electric fields. 2009 IEEE Pulsed Power Conference: June 28-July 2, 876-879, 2009.