Screening of cell-penetrating peptides using mRNA display

Biotechnology Journal

Volumn 7, Issue 3, 2012, Pages 387-396

  Lee, Jae Hun ^a   Song, Hyun Seok ^a   Lee, Sun Gu ^b   Park, Tae Hyun ^a   Kim, Byung Gee ^a  

^a Seoul National University   (South Korea)

^b Pusan National University   (South Korea)

Author keywords

Cell penetrating peptide; MRNA display; Peptide screening

Indexed keywords

CELL LINES; CELL PENETRATION; CELL-PENETRATING PEPTIDE; CELLULAR UPTAKE; HEK-293; HELA CELL; HUMAN EMBRYONIC KIDNEYS; IN-VITRO; IN-VIVO; MRNA-DISPLAY; PEPTIDE SCREENING; POLYARGININE; SCREENING METHODS; TARGET CELLS;

CELL CULTURE; DIAGNOSIS; PEPTIDES;

CELLS;

CELL PENETRATING PEPTIDE; MESSENGER RNA;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CELL-PENETRATING PEPTIDES; FLUORESCEIN-5-ISOTHIOCYANATE; GENETIC VECTORS; HEK293 CELLS; HELA CELLS; HUMANS; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 84863237869     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201100220     Document Type: Article

References (27)

1. Frankel, A. D., Pabo, C. O., Cellular uptake of the tat protein from human immunodeficiency virus. Cell 1988, 55, 1189-1193.
2. Green, M., Loewenstein, P. M., Autonomous functional domains of chemically synthesized human immunodeficiency virus tat trans-activator protein. Cell 1988, 55, 1179-1188.
3. Reynolds, F., Weissleder, R., Josephson, L., Protamine as an efficient membrane-translocating peptide. Bioconjug. Chem. 2005, 16, 1240-1245.
4. Boisseau, S., Mabrouk, K., Ram, N., Garmy, N. et al., Cell penetration properties of maurocalcine, a natural venom peptide active on the intracellular ryanodine receptor. Biochim. Biophys. Acta 2006, 1758, 308-319.
5. Pooga, M., Hallbrink, M., Zorko, M., Langel, U., Cell penetration by transportan. FASEB J. 1998, 12, 67-77.
6. Andaloussi, S., Johansson, H. J., Holm, T., Langel, U., A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids. Mol. Ther. 2005, 15, 1820-1826.
7. 4 peptides: structure-activity studies. Nucleic Acids Res. 2008, 36, 6343-6354.
8. Stewart, K. M., Horton, K. L., Kelley, S. O., Cell-penetrating peptides as delivery vehicles for biology and medicine. Org. Biomol. Chem. 2008, 6, 2242-2255.
9. Andaloussi, S. E., Guterstam, P., Langel, U., Assessing the delivery efficacy and internalization route of cell-penetrating peptides. Nat. Protoc. 2007, 2, 2043-2047.
10. Jones, S. W., Christison, R., Bundell, K., Voyce, C. J. et al., Characterization of cell-penetrating peptide-mediated peptide delivery. Br. J. Pharmacol. 2005, 145, 1093-1102.
11. Gao, C., Mao, S., Ditzel, H. J., Farnases, L. et al., A cell-penetrating peptide from a novel pVII-pIX phage-displayed random peptide library. Bioorg. Med. Chem. 2002, 10, 4057-4065.
12. Chen, Y., Shen, Y., Guo, X., Zhang, C. et al., Transdermal protein delivery by a coadministered peptide identified via phage display. Nat. Biotechnol. 2006, 24, 455-460.
13. Kamada, H., Okamoto, T., Kawamura, M., Shibata, H. et al., Creation of novel cell-penetrating peptides for intracellular drug delivery using systematic phage display technology originated from Tat transduction domain. Biol. Pharm. Bull. 2007, 30, 218-223.
14. Roberts, R. W., Szostak, J. W., RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc. Natl. Acad. Sci. USA 1997, 94, 12297-12302.
15. Liu, R., Barrick, J. E., Szostak, J. W., Roberts, R. W., Optimized synthesis of RNA-protein fusions for in vitro protein selection. Methods Enzymol. 2000, 318, 268-293.
16. Astriab-Fisher, A., Sergueev, D., Fisher, M., Shaw, B. R. et al., Conjugates of antisense oligonucleotides with the Tat and antennapedia cell-penetrating peptides: effects on cellular uptake, binding to target sequences, and biologic actions. Pharm. Res. 2002, 19, 744-754.
17. Muñoz, V., Serrano, L., Elucidating the folding problem of helical peptides using empirical parameters. II. Helix macrodipole effects and rational modification of the helical content of natural peptides. J. Mol. Biol. 1994, 245, 275-296.
18. Kaur, H., Garg, A., Raghava, G. P. S., PEPstr: A de novo method for tertiary structure prediction of small bioactive peptides. Protein Pept. Lett. 2007, 14, 626-631.
19. Esposito, C., Caputo, I., Mammalian transglutaminases. Identification of substrates as a key to physiological function and physiopathological relevance. FEBS J. 2005, 272, 615-631.
20. Herce, H. D., Garcia, A. E., Cell penetrating peptides: How do they do it? J. Biol. Phys. 2007, 33, 345-356.
21. Yamada, T., Fialho, A. M., Punj, V., Bratescu, L. et al., Internalization of bacterial redox protein azurin in mammalian cells: Entry domain and specificity. Cell Microbiol. 2005, 7, 1418-1431.
22. Taylor, B. N., Mehta, R. R., Yamada, T., Lekmine, F. et al., Noncationic peptides obtained from azurin preferentially enter cancer cells. Cancer Res. 2009, 69, 537-546.
23. Kamide, K., Nakakubo, H., Uno, S., Fukamizu, A., Isolation of novel cell-penetrating peptides from a random peptide library using in vitro virus and their modifications. Int. J. Mol. Med. 2010, 25, 41-51.
24. Herce, H. D., Garcia, A. E., Molecular dynamics simulations suggest a mechanism for translocation of the HIV-1 TAT peptide across lipid membranes. Proc. Natl. Acad. Sci. USA 2007, 104, 20805-20810.
25. Eguchi, A., Meade, B. R., Chang, Y-C., Fredrickson, C. T. et al., Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein. Nat. Biotechnol. 2009, 27, 567-571.
26. Lundberg, M., Wikstrom, S., Johansson, M., Cell surface adherence and endocytosis of protein transduction domains. Mol. Ther. 2003, 8, 143-150.
27. Sugahara, K. N., Teesalu, T., Karmali, P. P., Kotamraju, V. R. et al., Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs. Science 2010, 328, 1031-1035.