Functional and heterologous expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 in zebrafish

Journal of Bioscience and Bioengineering

Volumn 114, Issue 2, 2012, Pages 237-239

  Avşar Ban, Eriko ^a   Ishikawa, Hisayoshi ^a   Akiyama, Shin'ichi ^b   Manya, Hiroshi ^c   Endo, Tamao ^c   Tamaru, Yutaka ^a  

^a MIE UNIVERSITY   (Japan)

^b NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^c Tokyo Metropolitan Institute of Gerontology   (Japan)

Author keywords

Gene expression system; Hatching enzyme 1 promoter; Membrane associated protein; O mannosylation; Protein O linked mannose 1,2 N acetylglucosaminyltransferase 1 (POMGnT1); Zebrafish

Indexed keywords

EXPRESSION SYSTEM; HATCHING ENZYME 1 PROMOTER; MEMBRANE-ASSOCIATED PROTEINS; O-LINKED; O-MANNOSYLATION; ZEBRAFISH;

ESCHERICHIA COLI; GENE EXPRESSION;

PROTEINS;

ENHANCED GREEN FLUORESCENT PROTEIN; HUMAN PROTEIN O LINKED MANNOSE BETA 1,2 N ACETYLGLUCOSAMINYLTRANSFERASE 1; N ACETYLGLUCOSAMINYLTRANSFERASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; EXPRESSION VECTOR; HETEROLOGOUS EXPRESSION; HUMAN; HUMAN CELL; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; WESTERN BLOTTING; ZEBRA FISH;

ANIMALS; EMBRYO, NONMAMMALIAN; GENETIC VECTORS; HUMANS; MEMBRANE PROTEINS; N-ACETYLGLUCOSAMINYLTRANSFERASES; ZEBRAFISH;

BACTERIA (MICROORGANISMS); DANIO RERIO; ESCHERICHIA COLI;

EID: 84863198089     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2012.03.013     Document Type: Article

References (8)

1. Ellett F., Lieschke G.J. Zebrafish as a model for vertebrate hematopoiesis. Curr. Opin. Pharmacol. 2010, 10:563-570.
2. Kwan K.M., Otsuna H., Kidokoro H., Carney K.R., Saijoh Y., Chien C.B. A complex choreography of cell movements shapes the vertebrate eye. Development 2012, 139:359-372.
3. Sipes N.S., Parilla S., Knudsen T.B. Zebrafish: as an integrative model for twenty-first century toxicity testing. Birth Defects Res. C Embryo Today 2011, 93:256-267.
4. Yoshida A., Kobayashi K., Manya H., Taniguchi K., Kano H., Mizuno M., Inazu T., Mitsuhashi H., Takahashi S., Takeuchi M., other 7 authors Muscular dystrophy and neuronal migration disorder caused by mutations in a glycosyltransferase, POMGnT1. Dev. Cell 2001, 1:717-724.
5. Akasaka-Manya K., Manya H., Mizuno M., Inazu T., Endo T. Effects of length and amino acid sequence of O-mannosyl peptides on substrate specificity of protein O-linked mannose β1,2-N-acetylglucosaminyltransferase 1 (POMGnT1). Biochem. Biophys. Res. Commun. 2011, 410:632-636.
6. Voglmeir J., Kaloo S., Laurent N., Meloni M.M., Bohlmann L., Wilson I.B., Flitsch S.L. Biochemical correlation of activity of the α-dystroglycan-modifying glycosyltransferase POMGnT1 with mutations in muscle-eye-brain disease. Biochem. J. 2011, 436:447-455.
7. Moore C.J., Goh H.T., Hewitt J.E. Genes required for functional glycosylation of dystroglycan are conserved in zebrafish. Genomics 2008, 92:159-167.
8. Ichimiya T., Manya H., Ohmae Y., Yoshida H., Takahashi K., Ueda R., Endo T., Nishihara S. The twisted abdomen phenotype of Drosophila POMT1 and POMT2 mutants coincides with their heterophilic protein O-mannosyltransferase activity. J. Biol. Chem. 2004, 279:42638-42647.