W::Neo: A novel dual-selection marker for high efficiency gene targeting in Drosophila

PLoS ONE

Volumn 7, Issue 2, 2012, Pages

  Zhou, Wenke ^a   Huang, Juan ^a,b   Watson, Annie M ^a   Hong, Yang ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b NATIONAL INSTITUTE OF BIOLOGICAL SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HYBRID PROTEIN; NEOMYCIN; EYE PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; DNA INTEGRATION; DNA MODIFICATION; DROSOPHILA; EYE COLOR; GENE TARGETING; GENETIC ENGINEERING; GENETIC SELECTION; HOMOLOGOUS RECOMBINATION; KNOCKOUT GENE; MARKER GENE; NONHUMAN; ANIMAL; GENETIC MARKER; GENETICS; METHODOLOGY; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DROSOPHILA; EYE PROTEINS; GENE TARGETING; GENETIC ENGINEERING; GENETIC MARKERS; HOMOLOGOUS RECOMBINATION;

EID: 84863136795     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0031997     Document Type: Article

References (14)

1. Huang J, Zhou W, Dong W, Hong Y, (2009) Targeted engineering of the Drosophila genome. Fly (Austin) 3: 274-277.
2. Huang J, Zhou W, Dong W, Watson AM, Hong Y, (2009) Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering. Proc Natl Acad Sci U S A 106: 8284-8289.
3. Huang J, Ghosh P, Hatfull GF, Hong Y, (2011) Successive and Targeted DNA Integrations in Drosophila Genome by Bxb1 and phiC31 Integrases. Genetics 189: 391-395.
4. Gao G, McMahon C, Chen J, Rong YS, (2008) A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila. Proc Natl Acad Sci U S A 105: 13999-14004.
5. Choi CM, Vilain S, Langen M, Van Kelst S, De Geest N, et al. (2009) Conditional Mutagenesis in Drosophila. Science 324: 54-.
6. Weng R, Chen Y-W, Bushati N, Cliffe A, Cohen SM, (2009) Recombinase-Mediated Cassette Exchange Provides a Versatile Platform for Gene Targeting: Knockout of miR-31b. Genetics 183: 399-402.
7. Huang J, Zhou W, Watson AM, Jan Y-N, Hong Y, (2008) Efficient Ends-Out Gene Targeting In Drosophila. Genetics 180: 703-707.
8. Rong YS, Golic KG, (2000) Gene targeting by homologous recombination in Drosophila. Science 288: 2013-2018.
9. Gong WJ, Golic KG, (2003) Ends-out, or replacement, gene targeting in Drosophila. Proc Natl Acad Sci U S A 100: 2556-2561.
10. Beumer KJ, Trautman JK, Bozas A, Liu J-L, Rutter J, et al. (2008) Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc Natl Acad Sci U S A 105: 19821-19826.
11. Ramirez CL, Foley JE, Wright DA, Muller-Lerch F, Rahman SH, et al. (2008) Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Meth 5: 374-375.
12. Steller H, Pirrotta V, (1985) A transposable P vector that confers selectable G418 resistance to Drosophila larvae. Embo J 4: 167-171.
13. Schmucker D, Clemens JC, Shu H, Worby CA, Xiao J, et al. (2000) Drosophila Dscam Is an Axon Guidance Receptor Exhibiting Extraordinary Molecular Diversity. Cell 101: 671-684.
14. Xu T, Rubin GM, (1993) Analysis of genetic mosaics in developing and adult Drosophila tissues. Development 117: 1223-1237.