Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 8, 2012, Pages 2919-2924

  Botcherby, Edward J ^a   Smith, Christopher W ^a   Kohl, Michael M ^a,b   Deb́arre, Delphine ^c   Booth, Martin J ^a   Juškaitis, Rimas ^a   Paulsen, Ole ^a,b   Wilson, Tony ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c LABORATOIRE D OPTIQUE ET BIOSCIENCES   (France)

Author keywords

Fluorescence microscopy; Multiphoton imaging; Multiphoton microscopy; Three dimensional microscopy

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL SIZE; CONTROLLED STUDY; CYTOARCHITECTURE; MOUSE; MULTIPHOTON MICROSCOPY; NERVE CELL; NEUROIMAGING; NONHUMAN; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

ANIMALS; CEREBRAL CORTEX; IMAGING, THREE-DIMENSIONAL; MICE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; NEURONS; REPRODUCIBILITY OF RESULTS;

EID: 84863130942     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1111662109     Document Type: Article

References (23)

1. Denk W, Strickler JH, Webb WW (1990) Two-photon laser scanning fluorescence microscopy. Science 248:73-76.
2. Svoboda K, DenkW, Kleinfeld D, Tank DW (1997) In vivo dendritic calcium dynamics in neocortical pyramidal neurons. Nature 385:161-165. (Pubitemid 27034217)
3. Stosiek C, Garaschuk O, Holthoff K, Konnerth A (2003) In vivo two-photon calcium imaging of neuronal networks. Proc Natl Acad Sci USA 100:7319-7324. (Pubitemid 36706436)
4. Helmchen F, Denk W (2005) Deep tissue two-photon microscopy. Nat Methods 2:932-940. (Pubitemid 43108729)
5. Svoboda K, Yasuda R (2006) Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 50:823-839. (Pubitemid 43850693)
6. Göbel W, Kampa BM, Helmchen F (2007) Imaging cellular network dynamics in three dimensions using fast 3D laser scanning. Nat Methods 4:73-79. (Pubitemid 46029479)
7. Göbel W, Helmchen F (2007) New angles on neuronal dendrites in vivo. J Neurophysiol 98:3770-3779.
8. Grewe BF, Voigt FF, van't Hoff M, Helmchen F (2011) Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens. Biomed Opt Express 2:2035-2046.
9. Botcherby EJ, Juškaitis R, Booth MJ, Wilson T (2007) Aberration-free optical refocusing in high numerical aperture microscopy. Opt Lett 32:2007-2009. (Pubitemid 351389956)
10. Yuste R, Konnerth A (2005) Imaging in neuroscience and development. A laboratory manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York).
11. Born M, Wolf E (1999) Principles of optics: Electromagnetic theory of propagation, interference and diffraction of light (Cambridge University Press, Cambridge, UK), (7th edition).
12. Gross H, Zügge H, Peschka M, Blechinger F (2007) Handbook of Optical Systems, Volume 3, Aberration Theory and Correction of Optical Systems (Fig 33-15) (Wiley VCH, Weinhein, Germany).
13. Botcherby EJ, Juškaitis R, Booth MJ, Wilson T (2008) An optical technique for remote focusing in microscopy. Opt Commun 281:880-887.
14. Reddy GD, Kelleher K, Fink R, Saggau P (2008) Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity. Nat Neurosci 11:713-720. (Pubitemid 351748113)
15. Grewe BF, Langer D, Kasper H, Kampa BM, Helmchen F (2010) High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision. Nat Methods 7:399-405.
16. Saggau P (2006) New methods and uses for fast optical scanning. Curr Opin Neurobiol 16:543-550.
17. Kirkby PA, Naga Srinivas Nadella KM, Silver RA (2010) A compact acousto-optic lens for and 3D femtosecond based 2-photon microscopy. Opt Express 18:13720-13744.
18. Smith CW, Botcherby EJ, Booth MJ, Juškaitis R, Wilson T (2011) Agitation-free multiphoton microscopy of oblique planes. Opt Lett 36:663-665.
19. Smith CW, Botcherby EJ, Wilson T (2011) Resolution of oblique-plane images in sectioning microscopy. Opt Express 19:2662-2669.
20. Hoover EE, et al. (2010) Remote focusing for programmable multi-layer differential multiphoton microscopy. Biomed Opt Express 2:113-122.
21. Grewe BF, Helmchen F (2009) Optical probing of neuronal ensemble activity. Curr Opin Neurobiol 19:520-529.
22. Dodt HU, Zieglgänsberger W (1990) Visualizing unstained neurons in living brain slices by infrared DIC-videomicroscopy. Brain Res 537:333-336.
23. Garaschuk O, Milos RI, Konnerth A (2006) Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo. Nat Protoc 1:380-386.