Transmitochondrial cybrids: Tools for functional studies of mutant mitochondria

Methods in Molecular Biology

Volumn 837, Issue , 2012, Pages 219-230

  Vithayathil, Sajna Antony ^a   Ma, Yewei ^a   Kaipparettu, Benny Abraham ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Cybrids; Mitochondria; Mitochondrial depletion; Mitochondrial DNA mutation; Mitochondrial retrograde regulation; mtDNA; Rho0 cells; Transmitochondrial cybrids

Indexed keywords

ETHIDIUM; MITOCHONDRIAL DNA; URIDINE;

ARTICLE; CELL FUSION; GENETIC MARKER; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MITOCHONDRION; MUTATION;

CELL FUSION; DNA, MITOCHONDRIAL; ETHIDIUM; GENETIC MARKERS; HUMANS; MITOCHONDRIA; MUTATION; TRANSFECTION; URIDINE;

EID: 84863011496     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-504-6_15     Document Type: Article

References (12)

1. Chen, J. Q., Cammarata, P. R., Baines, C. P., and Yager, J. D. (2009) Regulation of mitochondrial respiratory chain biogenesis by estrogens/ estrogen receptors and physiological, pathological and pharmacological implications. Biochim Biophys Acta 1793, 1540-1570.
2. Liu, Z., and Butow, R. A. (2006) Mitochondrial retrograde signaling. Annu Rev Genet 40, 159-185. (Pubitemid 44956783)
3. Ishikawa, K., Takenaga, K., Akimoto, M., Koshikawa, N., Yamaguchi, A., Imanishi, H., et al. (2008) ROS-generating mitochondrial DNA mutations can regulate tumor cell metastasis. Science 320, 661-664. (Pubitemid 351928347)
4. Petros, J. A., Baumann, A. K., Ruiz-Pesini, E., Amin, M. B., Sun, C. Q., Hall, J., et al. (2005) mtDNA mutations increase tumorigenicity in prostate cancer. Proc Natl Acad Sci USA 102, 719-724. (Pubitemid 40282732)
5. Ma, Y., Bai, R. K., Trieu, R., and Wong, L. J. Mitochondrial dysfunction in human breast cancer cells and their transmitochondrial cybrids. Biochim Biophys Acta 1797, 29-37.
6. King, M. P., and Attardi, G. (1989) Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science 246, 500-503. (Pubitemid 19273941)
7. Hayakawa, T., Noda, M., Yasuda, K., Yorifuji, H., Taniguchi, S., Miwa, I., et al. (1998) Ethidium bromide-induced inhibition of mitochondrial gene transcription suppresses glucose-stimulated insulin release in the mouse pancreatic beta-cell line betaHC9. J Biol Chem 273, 20300-20307. (Pubitemid 28377593)
8. Zylber, E., and Penman, S. (1969) Mitochondrial-associated 4 S RNA synthesis inhibition by ethidium bromide. J Mol Biol 46, 201-204.
9. Magda, D., Lecane, P., Prescott, J., Thiemann, P., Ma, X., Dranchak, P. K., et al. (2008) mtDNA depletion confers specific gene expression profiles in human cells grown in culture and in xenograft. BMC Genomics 9, 521.
10. Bacman, S. R., and Moraes, C. T. (2007) Transmitochondrial technology in animal cells. Methods Cell Biol 80, 503-524.
11. Moraes, C. T., Dey, R., and Barrientos, A. (2001) Transmitochondrial technology in animal cells. Methods Cell Biol 65, 397-412.
12. Bayona-Bafaluy, M. P., Manfredi, G., and Moraes, C. T. (2003) A chemical enucleation method for the transfer of mitochondrial DNA to rho(o) cells. Nucleic Acids Res 31, e98.