Development of an IPTG inducible expression vector adapted for Bacteroides fragilis

Plasmid

Volumn 68, Issue 2, 2012, Pages 86-92

  Parker, Anita C ^a   Jeffrey Smith, C ^a  

^a EAST CAROLINA UNIVERSITY   (United States)

Author keywords

Bacteroides fragilis; Expression vector; LacI

Indexed keywords

ISOPROPYL THIOGALACTOSIDE; LACI PROTEIN; PROTEIN; THIOREDOXIN; UNCLASSIFIED DRUG; XYLAN 1,4 BETA XYLOSIDASE;

ARTICLE; BACTEROIDES FRAGILIS; BINDING SITE; CFXA BETA LACTAMASE GENE; CONCENTRATION RESPONSE; EXPRESSION VECTOR; GENE; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEOBACTERIA; TETQ GENE; TRANSCRIPTION REGULATION; TRXA GENE;

BACTEROIDES FRAGILIS; BASE SEQUENCE; GENE EXPRESSION REGULATION, BACTERIAL; GENE ORDER; GENES, REPORTER; ISOPROPYL THIOGALACTOSIDE; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS, GENETIC; THIOREDOXINS;

ANIMALIA; BACTEROIDES; BACTEROIDES FRAGILIS; BACTEROIDETES PHYLUM; NEGIBACTERIA; PROTEOBACTERIA;

EID: 84862987256     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2012.03.002     Document Type: Article

References (30)

1. An D., Na C., Bielawski J., Hannun Y.A., Kasper D.L. Membrane sphingolipids as essential molecular signals for Bacteroides survival in the intestine. Proc. Natl Acad. Sci. USA 2011, 108:4666-4671.
2. Bacic M., Parker A.C., Stagg J., Whitley H.P., Wells W.G., Jacob L.A., Smith C.J. Genetic and structural analysis of the Bacteroides conjugative transposon CTn341. J. Bacteriol. 2005, 187:2858-2869.
3. Barkocy-Gallagher G.A., Foley J.W., Lantz M.S. Activities of the Porphyromonas gingivalis PrtP proteinase determined by construction of prtP-deficient mutants and expression of the gene in Bacteroides species. J. Bacteriol. 1999, 181:246-255.
4. Bayley D.P., Rocha E.R., Smith C.J. Analysis of cepA and other Bacteroides fragilis genes reveals a unique promoter structure. FEMS Microbiol. Lett. 2000, 193:149-154.
5. Chen S., Bagdasarian M., Kaufman M.G., Bates A.K., Walker E.D. Mutational analysis of the ompA promoter from Flavobacterium johnsoniae. J. Bacteriol. 2007, 189:5108-5118.
6. Comstock L.E., Tzianabos A.O. Abscesses. Persistent Bacterial Infections 2000, 397-408. ASM Press, Washington DC. J.P. Nataro, M.J. Blaser, S. Cunningham-Rundles (Eds.).
7. Eckburg P.B., Bik E.M., Bernstein C.N., Purdom E., Dethlefsen L., Sargent M., Gill S.R., Nelson K.E., Relman D.A. Diversity of the human intestinal microbial flora. Science 2005, 308:1635-1638.
8. Finegold S.M., George W.L. Anaerobic Infections in Humans 1989, Academic Press, New York.
9. Karlsson F.H., Ussery D.W., Nielsen J., Nookaew I. A closer look at Bacteroides: phylogenetic relationship and genomic implications of a life in the human gut. Microb. Ecol. 2011, 61:473-485.
10. Khan S.R., Gaines J., Roop R.M., Farrand S.K. Broad-host-range expression vectors with tightly regulated promoters and their use to examine the influence of TraR and TraM expression on Ti plasmid quorum sensing. Appl. Environ. Microbiol. 2008, 74:5053-5062.
11. 4. Nature 1970, 227:680-685.
12. Lassmann B., Gustafson D.R., Wood C.M., Rosenblatt J.E. Reemergence of anaerobic bacteremia. Clin. Infect. Dis. 2007, 44:895-900.
13. Ley R.E., Turnbaugh P.J., Klein S., Gordon J.I. Microbial ecology: human gut microbes associated with obesity. Nature 2006, 444:1022-1023.
14. Lobo L.A., Smith C.J., Rocha E.R. Flavin mononucleotide (FMN)-based fluorescent protein (FbFP) as reporter for gene expression in the anaerobe Bacteroides fragilis. FEMS Microbiol. Lett. 2011, 317:67-74.
15. Mastropalol M.D., Thorson M.L., Stevens A.M. Comparison of Bacteroides thetaiotaomicron and Escherichia coli 16S rRNA gene expression signals. Microbiology 2009, 155:2683-2693.
16. Oehler S., Eismann E.R., Kramer H., Muller-Hill B. The three operators of the lac operon cooperate in repression. EMBO J. 1990, 9:973-979.
17. Parker A.C., Smith C.J. Genetic and biochemical analysis of a novel Ambler class A beta-lactamase responsible for cefoxitin resistance in Bacteroides species. Antimicrob. Agents Chemother. 1993, 37:1028-1036.
18. Paster B.J., Dewhirst F.E., Olsen I., Fraser G.J. Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria. J. Bacteriol. 1994, 176:725-732.
19. Privitera G., Dublanchet A., Sebald M. Transfer of multiple antibiotic resistance between subspecies of Bacteroides fragilis. J. Infect. Dis. 1979, 139:97-101.
20. Rasmussen B.A., Gluzman Y., Tally F.P. Escherichia coli chromosomal mutations that permit direct cloning of the Bacteroides fragilis metallo-β-lactamase gene, ccrA. Mol. Microbiol. 1991, 5:1211-1219.
21. Reott M.A., Parker A.C., Rocha E.R., Smith C.J. Thioredoxins in redox maintenance and survival during oxidative stress of Bacteroides fragilis. J. Bacteriol. 2009, 191:3384-3391.
22. Smith C.J., Rogers M.B., McKee M.L. Heterologous gene expression in Bacteroides fragilis. Plasmid 1992, 27:141-154.
23. Smith C.J., Rollins L.A., Parker A.C. Nucleotide sequence determination and genetic analysis of the Bacteroides plasmid, pBI143. Plasmid 1995, 34:211-222.
24. Smith C.J., Rocha E.R., Paster B.J. The medically important Bacteroides spp. in health and disease. The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community 2006, vol. 7:381-427. Springer Verlag, New York.
25. Spence C., Wells W.G., Smith C.J. Characterization of the primary starch utilization operon in the obligate anaerobe Bacteroides fragilis: regulation by carbon source and oxygen. J. Bacteriol. 2006, 188:4663-4672.
26. 2: the role of OxyR and its effect on survival and virulence. Mol. Microbiol. 2008, 67:129-142.
27. Vingadassalom D., Kolb A., Mayer C., Rybkine R., Collatz E., Prodglajen I. An unusual primary sigma factor in the Bacteroidetes phylum. Mol. Microbiol. 2005, 56:888-902.
28. Wang Y., Rotman E.R., Shoemaker N.B., Salyers A.A. Translational control of tetracycline resistance and conjugation in the Bacteroides Conjugative transposon CTnDOT. J. Bacteriol. 2005, 187:2673-2680.
29. Whitehead T.R. Development of a bifunctional xylosidase/arabinosidase gene as a reporter gene for the gram-negative anaerobes Bacteroides and Porphyromonas, and Escherichia coli. Curr. Microbiol. 1997, 35:282-286.
30. Wu G.D., Chen J., Hoffman C., Bittinger K., Chen Y.-Y., Keilbaugh S.A., Bewtra M., Knights D., Walters W.A., Knight R., Sinha R., Gilroy E., Gupta K., Baldassano R., Nessel L., Hongzhe L., Bushman F.D., Lweis J.D. Linking long-term dietary patterns with gut microbial enterotypes. Science 2011, 334:105-108.