Novel iron-sulfur containing NADPH-Reductase from Nocardia farcinica IFM10152 and fusion construction with CYP51 lanosterol demethylase

Biotechnology and Bioengineering

Volumn 109, Issue 3, 2012, Pages 630-636

  Choi, Kwon Young ^a   Jung, Eun Ok ^a   Jung, Da Hye ^a   Pandey, Bishnu Prasad ^a   Lee, Nahum ^a   Yun, Hyungdon ^b   Park, Hyung Yun ^a   Kim, Byung Gee ^a  

^a Seoul National University   (South Korea)

^b Yeungnam University   (South Korea)

Author keywords

CYP51; Fusion protein; Iron sulfur containing flavoprotein; Lanosterol demethylase

Indexed keywords

CYP51; CYTOCHROME P450; DEMETHYLASE; DEMETHYLATION; FUSION ENZYMES; FUSION PROTEIN; IRON-SULFUR CONTAINING FLAVOPROTEIN; KEY ENZYMES; LANOSTEROL; LINKER SEQUENCE; MONOOXYGENASES; NADPH-P450 REDUCTASE; NOCARDIA; RECOMBINANT ESCHERICHIA COLI; REDOX PARTNERS; SEQUENCE ALIGNMENTS; SPECTRAL PROPERTIES; STEROL BIOSYNTHESIS;

ALCOHOLS; BIOCHEMISTRY; ENZYME ACTIVITY; ESCHERICHIA COLI; SULFUR;

IRON;

CYTOCHROME P450; HYBRID PROTEIN; IRON SULFUR PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE FERRIHEMOPROTEIN REDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; STEROL 14ALPHA DEMETHYLASE;

ARTICLE; BACILLUS MEGATERIUM; CATALYSIS; CONTROLLED STUDY; DEMETHYLATION; ELECTRON TRANSPORT; FUSARIUM OXYSPORUM; GAS CHROMATOGRAPHY; GENE CLUSTER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; NOCARDIA FARCINICA; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS;

ESCHERICHIA COLI; GENE EXPRESSION; IRON-SULFUR PROTEINS; KINETICS; LANOSTEROL; NADP; NADPH-FERRIHEMOPROTEIN REDUCTASE; NOCARDIA; RECOMBINANT FUSION PROTEINS; SPECTRUM ANALYSIS; STEROL 14-DEMETHYLASE;

ESCHERICHIA COLI; NOCARDIA FARCINICA;

EID: 84862968306     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24359     Document Type: Article

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