SiRNA-mediated tissue factor knockdown in porcine neonatal islet cell clusters in vitro

Journal of Central South University (Medical Sciences)

Volumn 36, Issue 12, 2011, Pages 1141-1146

  Ji, Ming ^a   Yi, Shounan ^a,b   Yu, Deling ^a   Wang, Wei ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

^b UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Neonatal porcine islet cell cluster; Small interfering RNA; Tissue factor

Indexed keywords

SMALL INTERFERING RNA; THROMBOPLASTIN;

ANIMAL; ARTICLE; CELL CULTURE; CYTOLOGY; FEMALE; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; MALE; METABOLISM; NEWBORN; PANCREAS ISLET; REAL TIME POLYMERASE CHAIN REACTION; SWINE;

ANIMALS; ANIMALS, NEWBORN; CELLS, CULTURED; FEMALE; GENE KNOCKDOWN TECHNIQUES; GENE SILENCING; ISLETS OF LANGERHANS; MALE; REAL-TIME POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; SWINE; THROMBOPLASTIN; TRANSFECTION;

EID: 84862958952     PISSN: 16727347     EISSN: None     Source Type: Journal    
DOI: 10.3969/j.issn.1672-7347.2011.12.003     Document Type: Article

References (18)

1. Bennet W, Groth C G, Larsson R, et al. Isolated human islets trigger an instant blood mediated inflammatory reaction : implications for intraportal islet transplantation as a treatment for patients with type 1 diabetes[J]. Ups J Med Sci, 2000, 105 (2) : 125-133.
2. Bennet W, Sundberg B, Lundgren T, et al. Damage to porcine islets of Langerhans after exposure to human blood in vitro, or after intraportal transplantation to cynomologus monkeys : protective effects of sCRl and heparin [J]. Transplantation, 2000, 69 (5) : 711-719. (Pubitemid 30174086)
3. Cantarovich D, Blancho G, Potiron N, et al. Rapid failure of pig islet transplantation in non human primates[J]. Xenotransplantation, 2002, 9 (1) : 25-35. (Pubitemid 36358221)
4. Bennet W, Sundberg B, Groth C G, et al. Incompatibility between human blood and isolated islets of Langerhans : a finding with implications for clinical intraportal islet transplantation? [J]. Diabetes, 1999, 48 (10) : 1907-1914. (Pubitemid 29458351)
5. Moberg L. The role of the innate immunity in islet transplantation[J]. Ups J Med Sci, 2005, 110 (1) :17-55. (Pubitemid 40342459)
6. Ozmen L, Ekdahl K N, Elgue G, et al. Inhibition of thrombin abrogates the instant blood-mediated inflammatory reaction triggered by isolated human islets: possible application of the thrombin inhibitor melagatran in clinical islet transplantation [J]. Diabetes, 2002, 51 (6) : 1779-1784. (Pubitemid 34564290)
7. Cowan PJ, d'Apice A J. The coagulation barrier in xenotransplantation: incompatibilities and strategies to overcome them [J]. Curr Opin Organ Transplant, 2008, 13 (2) : 178-183. (Pubitemid 351374257)
8. Crikis S, Cowan P J, d'Apice A J. Intravascular thrombosis in discordant xenotransplantation [J]. Transplantation, 2006,82 (9) : 1119-1123. (Pubitemid 44748010)
9. Cowan P J. Coagulation and the xenograft endothelium[J]. Xenotransplantation, 2007, 14 (1) : 7-12. (Pubitemid 46051366)
10. van der Windt D J, Bottino R, Casu A, et al. Rapid loss of intraportally transplanted islets : an overview of pathophysiology and preventive strategies[J]. Xenotransplantation, 2007, 14 (4) : 288-297. (Pubitemid 47174365)
11. Moberg L, Johansson H, Lukinius A, et al. Production of tissue factor by pancreatic islet cells as a trigger of detrimental thrombotic reactions in clinical islet transplantation [J]. Lancet, 2002, 360 (9350) : 2039-2045. (Pubitemid 36026584)
12. Johansson H, Lukinius A, Moberg L, et al. Tissue factor produced by the endocrine cells of the islets of Langerhans is associated with a negative outcome of clinical islet transplantation [J]. Diabetes, 2005, 54 (6) : 1755-1762. (Pubitemid 40770765)
13. Korbutt G S, Elliott JF, Ao Z, et al. Large scale isolation, growth, and function of porcine neonatal islet Cells[J]. J Clin Invest, 1996, 97 (9) : 2119-2129. (Pubitemid 26143967)
14. [ ] Lin C C, Chen D, McVey JH, et al. Expression of tissue factor and initiation of clotting by human platelets and monocytes after incubation with porcine endothelial Cells[J]. Transplantation, 2008, 86 (5) :702-709.
15. Lin C C, Cooper D K, Dorling A. Coagulation dysregulation as a barrier to xenotransplantation in the primate[J]. Transpl Immunol, 2009, 21 (2) : 75-80.
16. Norris L A. Blood coagulation[J]. Best Pract Res Clin Obstet Gynaecol, 2003, 17 (3) :369-383. (Pubitemid 36722165)
17. Berman D M, Cabrera O, Kenyon N M, et al. Interference with tissue factor prolongs intrahepatic islet allograft survival in a nonhuman primate marginal mass model [J]. Transplantation, 2007, 84 (3) : 308-315. (Pubitemid 47258317)
18. Nagata M, Mullen Y, Matsuo S, et al. Destruction of islet isografts by severe nonspecific inflammation [J]. Transplant Proc, 1990, 22 (2) : 855-856. (Pubitemid 20160606)