A novel plant cell bioproduction platform for high-yield secretion of recombinant proteins

Methods in Molecular Biology

Volumn 824, Issue , 2012, Pages 483-500

  Xu, Jianfeng ^a   Kieliszewski, Marcia J ^b  

^a ARKANSAS STATE UNIVERSITY   (United States)

^b OHIO UNIVERSITY   (United States)

Author keywords

Enhanced green fluorescence protein; Hydroxyproline O glycosylation; Interferon 2; Plant cell culture; Recombinant protein; Secreted protein

Indexed keywords

ALPHA INTERFERON; ENHANCED GREEN FLUORESCENT PROTEIN; GLYCOPROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; HYDROXYPROLINE; OLIGONUCLEOTIDE; PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; CELL CULTURE; CHEMICAL STRUCTURE; CYTOLOGY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETIC TRANSFORMATION; GENETICS; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; RHIZOBIUM RADIOBACTER; SECRETION; SOLUBILITY; TOBACCO; WESTERN BLOTTING;

AGROBACTERIUM TUMEFACIENS; AMINO ACID SEQUENCE; BASE SEQUENCE; BIOTECHNOLOGY; BLOTTING, WESTERN; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLYCOPROTEINS; GLYCOSYLATION; GREEN FLUORESCENT PROTEINS; HYDROXYPROLINE; INTERFERON-ALPHA; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PEPTIDES; PLASMIDS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TOBACCO; TRANSFORMATION, GENETIC;

MAMMALIA;

EID: 84862946867     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-433-9_26     Document Type: Article

References (21)

1. Ma, J. K. C., Drake, P. M. W., and Christou, P. (2003) The production of recombinant pharmaceutical proteins in plants, Nature Reviews Genetics 4, 794-805. (Pubitemid 37193271)
2. Fischer, R., Stoger, E., Schillberg, S., et al., (2004) Plant-based production of biopharmaceuticals, Current Opinion in Plant Biology 7, 152-158. (Pubitemid 38308050)
3. Streatfi eld, S. J. (2007) Approaches to achieve high-level heterologous protein production in plants, Plant Biotechnology Journal 5, 2-15. (Pubitemid 46006523)
4. Doran, P. M. (2000) Foreign protein production in plant tissue cultures, Current Opinion in Biotechnology 11, 199-204. (Pubitemid 30182316)
5. Hellwig, S., Drossard, J., Twyman, R. M., et al., (2004) Plant cell cultures for the production of recombinant proteins, Nature Biotechnology 22, 1415-1422. (Pubitemid 39482861)
6. Georgiev, M. I., Weber, J., and Maciuk, A. (2009) Bioprocessing of plant cell cultures for mass production of targeted compounds, Applied Microbiology and Biotechnology 83, 809-823.
7. Xu, J. F., Tan, L., Goodrum, K. J., et al., (2007) High-yields and extended serum half-life of human interferon alpha 2b expressed in tobacco cells as Arabinogalactan-protein fusions, Biotechnology and Bioengineering 97, 997-1008. (Pubitemid 47195298)
8. Xu, J. F., Okada, S., Tan, L., et al., (2010) Human growth hormone expressed in tobacco cells as an arabinogalactan-protein fusion glycoprotein has a prolonged serum life, Transgenic Res. In press, doi: 10.1007/s11248-11010- 19367-11248.
9. Tan, L., Leykam, J. F., and Kieliszewski, M. J. (2003) Glycosylation motifs that direct arabinogalactan addition to arabinogalactan-proteins, Plant Physiology 132, 1362-1369. (Pubitemid 36872886)
10. Kieliszewski, M. J. (2001) The latest hype on Hyp-O-glycosylation codes, Phytochemistry 57, 319-323. (Pubitemid 32448443)
11. Kieliszewski, M. J., and Shpak, E. (2001) Synthetic genes for the elucidation of glycosylation codes for arabinogalactan-proteins and other hydroxyproline-rich glycoproteins, Cellular and Molecular Life Sciences 58, 1386-1398. (Pubitemid 32964410)
12. Zhao, Z. D., Tan, L., Showalter, A. M., et al., (2002) Tomato LeAGP-1 arabinogalactanprotein purifi ed from transgenic tobacco corroborates the Hyp contiguity hypothesis, Plant Journal 31, 431-444. (Pubitemid 34989851)
13. Xu, J. F., Tan, L., Lamport, D. T. A., et al., (2008) The O-Hyp glycosylation code in tobacco and Arabidopsis and a proposed role of Hyp-glycans in secretion, Phytochemistry 69, 1631-1640.
14. Shpak, E., Barbar, E., Leykam, J. F., et al., (2001) Contiguous hydroxyproline residues direct hydroxyproline arabinosylation in Nicotiana tabacum, Journal of Biological Chemistry 276, 11272-11278. (Pubitemid 38089315)
15. Shpak, E., Leykam, J. F., and Kieliszewski, M. J. (1999) Synthetic genes for glycoprotein design and the elucidation of hydroxyproline- O-glycosylation codes, Proceedings of the National Academy of Sciences of the United States of America 96, 14736-14741. (Pubitemid 30019709)
16. De Loose, M., Gheysen, G., Tire, C., et al.,(1991) The extensin signal peptide allows secretion of a heterologous protein from protoplasts, Gene 99, 95-100.
17. Murashige, T., and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco cultures, Physiol. Plant 15, 473-497.
18. Schenk, R. U., and Hildebrandt, A. C. (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures, Can. J. Bot. 50, 199-204.
19. Mayo, K. J., Gonzales, B. J., and Mason, H. S. (2006) Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens, Nature Protocols 1, 1105-1111.
20. Osborn, B. L., Olsen, H. S., Nardelli, B., et al., (2002) Pharmacokinetic and pharmacodynamic studies of a human serum albumin-interferonalpha fusion protein in cynomolgus monkeys, Journal of Pharmacology and Experimental Therapeutics 303, 540-548. (Pubitemid 35231224)
21. Knight Jr, E., and Fahey, D. (1981) Human fi broblast interferon. An improved purifi cation, Journal of Biological Chemistry 256, 3609-3611. (Pubitemid 11089523)