Production, purification, and characterization of the cecropin from Plutella xylostella, pxCECA1, using an intein-induced self-cleavable system in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 94, Issue 4, 2012, Pages 1031-1039

  Wang, Hong ^a,b   Meng, Xiao Lin ^a   Xu, Jin Ping ^a   Wang, Jian ^a   Wang, Hua ^a   Ma, Chun Wei ^b  

^a WUHAN UNIVERSITY   (China)

^b ZHONGNAN HOSPITAL OF WUHAN UNIVERSITY   (China)

Author keywords

Antimicrobial activity; Cecropin; Plutella xylostella; Self cleavage

Indexed keywords

ANTI-MICROBIAL ACTIVITY; ANTIMICROBIAL DRUG; ANTIMICROBIAL PEPTIDE; C-TERMINUS; CECROPIN; CELL EXTRACTS; CHITIN-BINDING DOMAINS; COST-EFFECTIVE METHODS; E. COLI; EXPRESSION SYSTEM; FAT BODY; FUSION PROTEINS; GRAM-NEGATIVE BACTERIA; IMMUNE DEFENSE; INFECTIOUS AGENTS; MICROBICIDAL AGENTS; OPTIMAL CONDITIONS; PH-DEPENDENT; PLUTELLA XYLOSTELLA; SELF-CLEAVAGE; STAPHYLOCOCCUS AUREUS; THERAPEUTIC APPLICATION;

BACTERIA; CELL CULTURE; CHITIN; CLONING; COST EFFECTIVENESS; GENE ENCODING; PURIFICATION; VIRUSES;

ESCHERICHIA COLI;

CATALASE; CECROPIN; CELL EXTRACT; CHITIN; HYBRID PROTEIN; INTEIN; MICROBICIDE; POLYPEPTIDE ANTIBIOTIC AGENT;

ANTIMICROBIAL ACTIVITY; BIOASSAY; BIOLOGICAL PRODUCTION; CHITIN; COLIFORM BACTERIUM; COST-BENEFIT ANALYSIS; GENE EXPRESSION; IMMUNE RESPONSE; MOTH; PATHOGEN; PEPTIDE; PH; POLYMERASE CHAIN REACTION; PROTEIN; PURIFICATION; RECOMBINATION; TEMPERATURE EFFECT;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM CULTURE; COST EFFECTIVENESS ANALYSIS; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE SEQUENCE; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PROTEIN BINDING; PROTEIN EXPRESSION; PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; TEMPERATURE;

ANIMALS; CECROPINS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; HYDROGEN-ION CONCENTRATION; INTEINS; LEPIDOPTERA; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

ESCHERICHIA COLI; FUNGI; HEXAPODA; NEGIBACTERIA; PLUTELLA XYLOSTELLA; STAPHYLOCOCCUS AUREUS;

EID: 84862906434     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3863-5     Document Type: Article

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