Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC)

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 900, Issue , 2012, Pages 71-78

  Orr, Valerie ^a   Scharer, Jeno ^a   Moo Young, Murray ^a   Honeyman, C Howie ^b   Fenner, Drew ^b,d   Crossley, Lisa ^b,d   Suen, Shing Yi ^c   Chou, C Perry ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

^b Natrix Separations Inc   (Canada)

^c NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^d BioVenturesca   (Canada)

Author keywords

Anion exchange membrane chromatography; Extracellular secretion; One step purification; Penicillin G acylase; Tangential flow filtration

Indexed keywords

ANION EXCHANGE MEMBRANE; EXTRACELLULAR SECRETION; ONE-STEP PURIFICATION; PENICILLIN G ACYLASE; TANGENTIAL FLOW;

BIOLOGICAL MEMBRANES; CELL CULTURE; CLARIFICATION; CLARIFIERS; ESCHERICHIA COLI; IONS; MEMBRANES; MICROFILTRATION; PURIFICATION; RECOMBINANT PROTEINS;

CHROMATOGRAPHY;

ESCHERICHIA COLI PROTEIN; PENICILLIN AMIDASE; RECOMBINANT PROTEIN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACTERIUM CULTURE; BIOREACTOR; CROSSFLOW FILTRATION; ENZYME BINDING; ESCHERICHIA COLI; FILTRATION; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; TANGENTIAL FLOW FILTRATION;

ANIONS; BIOREACTORS; CHROMATOGRAPHY, ION EXCHANGE; ESCHERICHIA COLI; PENICILLIN AMIDASE; RECOMBINANT PROTEINS; ULTRAFILTRATION;

ESCHERICHIA COLI;

EID: 84862899840     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2012.05.039     Document Type: Article

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