Understanding and correcting for carbon nanotube interferences with a commercial LDH cytotoxicity assay

Toxicology

Volumn 299, Issue 2-3, 2012, Pages 99-111

  Wang, Gang ^a,b   Zhang, Jianping ^b   Dewilde, Abiche H ^b   Pal, Anoop K ^a   Bello, Dhimiter ^a   Therrien, Joel M ^c   Braunhut, Susan J ^b   Marx, Kenneth A ^a  

^a UNIVERSITY OF MASSACHUSETTS LOWELL   (United States)

^b University of Massachusetts Lowell   (United States)

^c University of Massachusetts   (United States)

Author keywords

LDH assay; Macrophage; Nanomaterials; Nanotoxicity; SWCNH ox; SWCNT

Indexed keywords

BOVINE SERUM ALBUMIN; HYDROGEN PEROXIDE OXIDIZED SINGLE WALLED CARBON NANOHORN; LACTATE DEHYDROGENASE; SINGLE WALLED NANOTUBE; UNCLASSIFIED DRUG;

ACCURACY; ANALYTICAL ERROR; ARTICLE; ARTIFACT; CHROMATOPHORE; CONCENTRATION RESPONSE; CYTOTOXICITY TEST; ENZYME ACTIVITY; MACROPHAGE; MOLECULAR PROBE; PRIORITY JOURNAL; SPECTROPHOTOMETRY;

ANIMALS; CELL LINE; DOGS; L-LACTATE DEHYDROGENASE; MACROPHAGES; MICROSCOPY, ELECTRON, TRANSMISSION; NANOTUBES, CARBON; SPECTROPHOTOMETRY, ULTRAVIOLET; TOXICITY TESTS;

EID: 84862851582     PISSN: 0300483X     EISSN: 18793185     Source Type: Journal    
DOI: 10.1016/j.tox.2012.05.012     Document Type: Article

References (46)

1. Andre N.D., Barbosa D.S., Munhoz E., Estevão D., Cecchini R., Watanabe M.A. Measurement of cytotoxic activity in experimental cancer. J. Clin. Lab. Anal. 2004, 18:27-30.
2. Asakura M., Sasaki T., Sugiyama T., Takaya M., Koda S., Nagano K., Arito H., Fukushima S. Genotoxicity and cytotoxicity of multi-wall carbon nanotubes in cultured Chinese hamster lung cells in comparison with chrysotile A fibers. J. Occup. Health 2010, 52:155-166.
3. Ayres J.G., Borm P., Cassee F.R., Castranova V., Donaldson K., Ghio A. Evaluating the toxicity of airborne particulate matter and nanoparticles by measuring oxidative stress potential - a workshop report and consensus statement. Inhal. Toxicol. 2008, 20:75-99.
4. Bello D., Hsieh S.-F., Schmidt D., Rogers E. Nanomaterials properties vs. biological oxidative damage: implications for toxicity screening and exposure assessment. Nanotoxicology 2009, 3:249-261.
5. Blauer G., King T.E. Interactions of bilirubin with bovine serum albumin in aqueous solution. J. Biol. Chem. 1970, 245:372-381.
6. Casey A., Davoren M., Herzog E., Lyng F.M., Byrne H.J., Chambers G. Probing the interaction of single walled carbon nanotubes within cell culture medium as a precursor to toxicity testing. Carbon 2007, 45:34-40.
7. Casey A., Herzog E., Davoren M., Lyng F.M., Byrne H.J., Chambers G. Spectroscopic analysis confirms the interactions between single walled carbon nanotubes and various dyes commonly used to assess cytotoxicity. Carbon 2007, 45:1425-1432.
8. Casey A., Herzog E., Lyng F., Byrne M., Chambers H.J., Davoren G.M. Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells. Toxicol. Lett. 2008, 179:78-84.
9. Curran S.A., Ellis A.V., Vijayaraghavan A., Ajayan P.M. Functionalization of carbon nanotubes using phenosafranin. J. Chem. Phys. 2004, 120:4886-4889.
10. Decker T., Lohmann-Matthes M.L. A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J. Immunol. Methods 1988, 115:61-69.
11. Doak S.H., Griffiths S.M., Manshian B., Singh N., Williams P.M., Brown A.P., Jenkins G.J. Confounding experimental considerations in nanogenotoxicology. Mutagenesis 2009, 24:285-293.
12. Gerloff K., Albrecht C., Boots A.W., Forster I., Schins R.P.F. Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells. Nanotoxicology 2009, 3:355-364.
13. Han S.G., Andrews R., Gairola C.G., Bhalla D.K. Acute pulmonary effects of combined exposure to carbon nanotubes and ozone in mice. Inhal. Toxicol. 2008, 20:391-398.
14. Han X., Gelein R., Corson N., Wade-Mercer P., Jiang J., Biswas P., Finkelstein J.N., Elder A., Oberdörster G. Validation of an LDH assay for assessing nanoparticle toxicity. Toxicology 2011, 287:99-104.
15. Hansen S.F., Larsen B.H., Stig I., Olsen S.I., Anders B.A. Categorization framework to aid hazard identification of nanomaterials. Nanotoxicology 2007, 1:243-250.
16. Jos A., Pichardo S., Puerto M., Sanchez E., Grilo A., Camean A.M. Cytotoxicity of carboxylic acid functionalized single wall carbon nanotubes on the human intestinal cell line Caco-2. Toxicol. In Vitro 2009, 23:1491-1496.
17. Kane A.B., Hurt R.H. Nanotoxicology: the asbestos analogy revisited. Nat. Nanotechnol. 2008, 3:378-379.
18. Kim J.S., Song K.S., Joo H.J., Lee J.H., Yu I.J. Determination of cytotoxicity attributed to multiwall carbon nanotubes (MWCNT) in normal human embryonic lung cell (WI-38) line. J. Toxicol. Environ. Health A 2010, 73:1521-1529.
19. Li P.R., Wei J.C., Chiu Y.F., Su H.L., Peng F.C., Lin J.J. Evaluation on cytotoxicity and genotoxicity of the exfoliated silicate nanoclay. ACS Appl. Mater. Inter. 2010, 2:1608-1613.
20. Li Y.S., Li Y.F., Li Q.N., Li J.G., Li J., Huang Q., Li W.X. The acute pulmonary toxicity in mice induced by multiwall carbon nanotubes, benzene, and their combination. Environ. Toxicol. 2010, 25:409-417.
21. Lin Z.Q., Xi Z.G., Chao F.H., Yang D.F., Zhang H.S., Lin B.C., Zhang W., Liu H.L., Sun X. ICAM-1 and VCAM-1 expression in rat aortic endothelial cells after single-walled carbon nanotube exposure. J. Nanosci. Nanotechnol. 2010, 10:8562-8574.
22. Loboa A.O., Corat M.A.F., Antunesa E.F., Palma M.B.S., Pacheco-Soares C., Corat E.J. Cytotoxicity analysis of vertically aligned multi-walled carbon nanotubes by colorimetric assays. Synth. Met. 2009, 159:2165-2166.
23. MacCormack T.J., Clark R.J., Dang M.K.M., Ma G., Kelly J.A., Veinot J.G.C., Goss G.G. Inhibition of enzyme activity by nanomaterials: potential mechanisms and implications for nanotoxicity testing. Nanotoxicology 2011, 1-12.
24. Maynard A.D. Oversight of engineered nanomaterials in the workplace. J. Law Med. Ethics 2009, 37:651-658.
25. Monteiro-Riviere N.A., Inman A.O., Zhang L.W. Limitations and relative utility of screening assays to assess engineered nanoparticle toxicity in a human cell line. Toxicol. Appl. Pharmacol. 2009, 234:222-235.
26. Nassimi M., Schleh C., Lauenstein H.D., Hussein R., Hoymann H.G., Koch W., Pohlmann G., Krug N., Sewald K., Rittinghausen S., Braun A., Muller-Goymann C. A toxicological evaluation of inhaled solid lipid nanoparticles used as a potential drug delivery system for the lung. Eur. J. Pharm. Biopharm. 2010, 75:107-116.
27. Pal, A.K., Bello, D., Budhlall, B., Rogers, E., Milton, D.K. Screening for oxidative stress elicited by engineered nanomaterials: evaluation of acellular DCFH assay. Dose-Response, submitted for publication.
28. Reddy A.R.N., Reddy Y.N., Himabindu V., Krishna D.R. Induction of oxidative stress and cytotoxicity by carbon nanomaterials is dependent on physical properties. Toxicol. Ind. Health 2010, 27:3-10.
29. Reddy A.R.N., Reddy Y.N., Krishna D.R., Himabindu V. Multi wall carbon nanotubes induce oxidative stress and cytotoxicity in human embryonic kidney (HEK293) cells. Toxicology 2010, 272:11-16.
30. Roberts J.E., Wielgus A.R., Boyes W.K., Andley U., Chignell C.F. Phototoxicity and cytotoxicity of fullerol in human lens epithelial cells. Toxicol. Appl. Pharmacol. 2008, 228:49-58.
31. Rogers E.J., Hsieh S.F., Organti N., Schmidt D.D.B. A high throughput in vitro analytical approach to screen for oxidative stress potential exerted by nanomaterials using a biologically relevant matrix: human blood serum. Toxicol. In Vitro 2008, 22:1639-1647.
32. Sun Y., Wilson S.R., Schuster D.I. High dissolution and strong light emission of carbon nanotubes in aromatic amine solvents. J. Am. Chem. Soc. 2001, 123:5348-5349.
33. Vorotnikova E., Rosenthal R., Tries M., Doctrow S., Braunhut S.J. Novel synthetic SOD mimetics can mitigate capillary endothelial cell apoptosis caused by ionizing radiation. Radiat. Res. 2010, 173:748-759.
34. Wako K., Kotani Y., Hirose A., Doi T., Hamada S. Effects of preparation methods for multi-wall carbon nanotube (MWCNT) suspensions on MWCNT induced rat pulmonary toxicity. J. Toxicol. Sci. 2010, 35:437-446.
35. Walker V.G., Li Z., Hulderman T., Schwegler-Berry D., Kashon M.L., Simeonova P.P. Potential in vitro effects of carbon nanotubes on human aortic endothelial cells. Toxicol. Appl. Pharmcol. 2009, 236:319-328.
36. Wang G., Dewilde A.H., Zhang J., Pal A.K., Vashist M., Bello D., Marx K.A., Braunhut S.J., Therrien J.M. A living cell quartz crystal microbalance biosensor for continuous monitoring of cytotoxic responses of macrophages to single-walled carbon nanotubes. Part Fibre Toxicol. 2011, 8:4.
37. Wang J.X., Zhou G.Q., Chen C.Y., Yu H.W., Wang T.C., Ma Y.M., Jia G., Gao Y.X., Li B., Sun J., Li Y.F., Jiao F., Zhao Y.L., Chai Z.F. Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. Toxicol. Lett. 2007, 168:176-185.
38. 2. Toxicol in Vitro 2011, 25:2147-2151.
39. Wang X., Zang J.J., Wang H., Nie H., Wang T.C., Deng X.Y., Gu Y.Q., Liu Z.H., Jia G. Pulmonary toxicity in mice exposed to low and medium doses of water-soluble multi-walled carbon nanotubes. J. Nanosci. Nanotechnol. 2010, 10:8516-8526.
40. Wielgus A.R., Zhao B., Chignell C.F., Hu D.N., Roberts J.E. Phototoxicity and cytotoxicity of fullerol in human retinal pigment epithelial cells. Toxicol. Appl. Pharmcol. 2010, 242:79-90.
41. Wohlleben W., Kolle S.N., Hasenkamp L.-C., Böser A., Vogel S., Vacano B.v., Ravenzwaay B.v., Landsiedel R. Artifacts by marker enzyme adsorption on nanomaterials in cytotoxicity assays with tissue cultures. J. Phys. Conf. Ser. 2011, 304:012061.
42. Worle-Knirsch J.M., Pulskamp K., Krug H.F. Oops they did it again! Carbon nanotubes hoax scientists in viability assays. Nano Lett. 2006, 6:1261-1268.
43. Yang S.T., Wang X., Jia G., Gu Y.Q., Wang T.C., Nie H.Y., Ge C.C., Wang H.F., Liu Y.F. Long-term accumulation and low toxicity of single-walled carbon nanotubes in intravenously exposed mice. Toxicol. Lett. 2008, 181:182-189.
44. Yudasaka M., Iijima S., Crespi V. Single-wall carbon nanohorns and nanocones. Carbon Nanotubes 2008, Springer, Berlin/Heidelberg, pp. 605-629.
45. Zhang J., Dewilde A.J., Chinn P., Barry S., Kanne D., Braunhut S.J. Herceptin-directed nanoparticles activated by an alternating magnetic field selectively kill Her-2 positive human breast in vitro, hyperthermia. Int. J. Hyperthermia 2011, 27:682-697.
46. Zhang J., Lee J.K., Wu Y., Murray R.W. Photoluminescence and electronic interaction of anthracene derivatives adsorbed on sidewalls of single-walled carbon nanotubes. Nano Lett. 2003, 3:403-407.