New homogeneous high-throughput assays for inhibitors of β-catenin/Tcf protein-protein interactions

Analytical Biochemistry

Volumn 424, Issue 1, 2012, Pages 57-63

  Zhang, Min ^a   Huang, Zheng ^a   Yu, Binxun ^a   Ji, Haitao ^a  

^a UNIVERSITY OF UTAH   (United States)

Author keywords

AlphaScreen; Fluorescence polarization; High throughput screening; Wnt signaling; Catenin Tcf interactions

Indexed keywords

DRUG PRODUCTS; FLUORESCENCE; POLARIZATION;

ALPHASCREEN; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT ASSAYS; PROTEIN-PROTEIN INTERACTIONS; SELECTIVE INHIBITION; TRANSCRIPTIONAL ACTIVATORS; WNT SIGNALING;

PROTEINS;

BETA CATENIN; T CELL FACTOR PROTEIN;

ALPHASCREEN; AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CHEMICAL ANALYSIS; CONCENTRATION (PARAMETERS); FLUORESCENCE POLARIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION;

EID: 84862824396     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.02.018     Document Type: Article

References (31)

1. P. Morin, A.B. Sparks, V. Korinek, N. Barker, H. Clevers, B. Vogelstein, and K.W. Kinzler Activation of β-catenin-Tcf signaling in colon cancer by mutations in β-catenin or APC Science 275 1997 1787 1790
2. A. de La Coste, B. Romagnolo, P. Billuart, C. Renard, M. Buendia, O. Soubrane, M. Fabre, J. Chelly, C. Beldjord, A. Kahn, and C. Perret Somatic mutations of the β-catenin gene are frequent in mouse and human hepatocellular carcinomas Proc. Natl. Acad. Sci. USA 95 1998 8847 8851
3. C. Scheel, E.N. Eaton, S.H. Li, C.L. Chaffer, F. Reinhardt, K. Kah, G. Bell, W. Guo, J. Rubin, A.L. Richardson, and R.A. Weinberg Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast Cell 145 2011 926 940
4. D. Lu, Y. Zhao, R. Tawatao, H.B. Cottam, M. Sen, L.M. Leoni, T.J. Kipps, M. Corr, and D.A. Carson Activation of the Wnt signaling pathway in chronic lymphocytic leukemia Proc. Natl. Acad. Sci. USA 101 2004 3118 3123
5. K. Sukhdeo, M. Mani, Y. Zhang, J. Dutta, H. Yasui, M.D. Rooney, D.E. Carrasco, M. Zheng, H. He, Y. Tai, C. Mitsiades, K.C. Anderson, and D.R. Carrasco Targeting the β-catenin/TCF transcriptional complex in the treatment of multiple myeloma Proc. Natl. Acad. Sci. USA 104 2007 7516 7521
6. W.R. Henderson Jr., E.Y. Chi, X. Ye, C. Nguyen, Y. Tien, B. Zhou, Z. Borok, D.A. Knight, and M. Kahn Inhibition of Wnt/β-catenin/CREB binding protein (CBP) signaling reverses pulmonary fibrosis Proc. Natl. Acad. Sci. USA 107 2010 14309 14314
7. J.H. Cheng, H. She, Y. Han, J. Wang, S. Xiong, K. Asahina, and H. Tsukamoto Wnt antagonism inhibits hepatic stellate cell activation and liver fibrosis Am. J. Physiol. Gastrointest. Liver Physiol. 294 2008 G39 G49
8. M.A. Lancaster, C.M. Louie, J.L. Silhavy, L. Sintasath, M. Decambre, S.K. Nigam, K. Willert, and J.G. Gleeson Impaired Wnt-β-catenin signaling disrupts adult renal homeostasis and leads to cystic kidney ciliopathy Nat. Med. 15 2009 1046 1054
9. H. Clevers Wnt/β-catenin signaling in development and disease Cell 127 2006 469 480
10. P. Polakis The many ways of Wnt in cancer Curr. Opin. Genet. Dev. 17 2007 45 51
11. -/- colon carcinoma Science 275 1997 1784 1787
12. T.-C. He, A.B. Sparks, C. Rago, H. Hermeking, L. Zawel, L.T. da Costa, P.J. Morin, B. Vogelstein, and K.W. Kinzler Identification of c-MYC as a target of the APC pathway Science 281 1998 1509 1512
13. O. Tetsu, and F. McCormick β-Catenin regulates expression of cyclin D1 in colon carcinoma cells Nature 398 1999 422 426
14. M. Shtutman, J. Zhurinsky, I. Simcha, C. Albanese, M. D'Amico, R. Pestell, and A. Ben-Ze'ev The cyclin D1 gene is a target of the β-catenin/LEF-1 pathway Proc. Natl. Acad. Sci. USA 96 1999 5522 5527
15. J.C. Tapia, V.A. Torres, D.A. Rodriguez, L. Leyton, and A.F. Quest Casein kinase 2 (CK2) increases surviving expression via enhanced β-catenin-T cell factor/lymphoid enhancer binding factor-dependent transcription Proc. Natl. Acad. Sci. USA 103 2006 15079 15084
16. F. Poy, M. Lepourcelet, R.A. Shivdasani, and M.J. Eck Structure of a human Tcf4-β-catenin complex Nat. Struct. Biol. 8 2001 1053 1057
17. T.A. Graham, D.M. Ferkey, F. Mao, D. Kimelman, and W. Xu Tcf4 can specifically recognize β-catenin using alternative conformations Nat. Struct. Biol. 8 2001 1048 1052
18. J. Sampietro, C.L. Dahlberg, U.S. Cho, T.R. Hinds, D. Kimelman, and W. Xu Crystal structure of a β-catenin/BCL9/Tcf4 complex Mol. Cell 24 2006 293 300
19. C.A. Omer, P.J. Miller, R.E. Diehl, and A.M. Kral Identification of Tcf4 residues involved in high-affinity β-catenin binding Biochem. Biophys. Res. Commun. 256 1999 584 590
20. S. Knapp, M. Zamai, D. Volpi, V. Nardese, N. Avanzi, J. Breton, S. Plyte, M. Flocco, M. Marconi, A. Isacchi, and V.R. Caiolfa Thermodynamics of the high-affinity interaction of TCF4 with β-catenin J. Mol. Biol. 306 2001 1179 1189
21. J.P. von Kries, G. Winbeck, C. Asbrand, T. Schwarz-Romond, N. Sochnikova, A. Dell'Oro, J. Behrens, and W. Birchmeier Hot spots in β-catenin for interactions with LEF-1, conductin and APC Nat. Struct. Biol. 7 2000 800 807
22. R. Gail, R. Frank, and A. Wittinghofer Systematic peptide array-based delineation of the differential β-catenin interaction with Tcf4, E-cadherin, and adenomatous polyposis coli J. Biol. Chem. 280 2005 7107 7117
23. Y. Xing, K.-I. Takemaru, J. Liu, J.D. Berndt, J.J. Zheng, R.T. Moon, and W. Xu Crystal structure of a full-length β-catenin Structure 16 2008 478 487
24. J. Sun, and W.I. Weis Biochemical and structural characterization of β-catenin interactions with nonphosphorylated and CK2-phosphorylated Lef-1 J. Mol. Biol. 405 2011 519 530
25. F.C. Gonsalves, K. Klein, B.B. Carson, S. Katz, L.A. Ekas, S. Evans, R. Nagourney, T. Cardozo, A.M. Brown, and R. DasGupta An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway Proc. Natl. Acad. Sci. USA 108 2011 5954 5963
26. M. Lepourcelet, Y.P. Chen, D.S. France, H. Wang, P. Crews, F. Petersen, C. Bruseo, A.W. Wood, and R.A. Shivdasani Small-molecule antagonists of the oncogenic Tcf/β-catenin protein complex Cancer Cell 5 2004 91 102
27. W. Tian, Y. Xu, X. Han, S. Duggineni, X. Han, Z. Huang, and J. An Development of a novel fluorescence polarization-based assay for studying the β-catenin/Tcf4 interaction J. Biomol. Screen. 17 2012 10.1177/ 1087057111429745
28. R.M. Eglen, T. Reisine, P. Roby, N. Rouleau, C. Illy, R. Bossé, and M. Bielefeld The use of AlphaScreen technology in HTS: current status Curr. Chem. Genomics 1 2008 2 10
29. J. Zhang, T.D.Y. Chung, and K.R. Oldenburg A simple statistical parameter for use in evaluation and validation of high throughput screening assays J. Biomol. Screen. 4 1999 67 73
30. N.J. Moerke Fluorescence polarization (FP) assays for monitoring peptide-protein or nucleic acid-protein binding Curr. Protoc. Chem. Biol. 1 2009 1 15
31. S. Kadhodayan, L.O. Elliott, G. Mausisa, H.A. Wallweber, K. Deshayes, B. Feng, and W.J. Fairbrother Evaluation of assay technologies for the identification of protein-protein interaction antagonists Assay Drug Dev. Technol. 5 2007 501 513