The small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells

Biochemical and Biophysical Research Communications

Volumn 420, Issue 2, 2012, Pages 417-421

  Sato, Mai ^a   Kitaguchi, Tetsuya ^b   Numano, Rika ^c   Ikematsu, Kazuya ^d   Kakeyama, Masaki ^e   Murata, Masayuki ^a   Sato, Ken ^a   Tsuboi, Takashi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b WASEDA UNIVERSITY   (Japan)

^c TOYOHASHI UNIVERSITY OF TECHNOLOGY   (Japan)

^d NAGASAKI UNIVERSITY   (Japan)

^e UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cdc42; Dense core vesicle; Exocytosis; GTPase; Live cell imaging; Neuroendocrine cells

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; NEURAL WISKOTT ALDRICH SYNDROME PROTEIN; PROTEIN CDC42; RAC PROTEIN; TUBA PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL FUSION; CELL STRAIN; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EXOCYTOSIS; IMMUNOCYTOCHEMISTRY; INHIBITION KINETICS; MEMBRANE VESICLE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; WILD TYPE;

DERRIS TRIFOLIATA;

EID: 84862813671     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.03.010     Document Type: Article

References (18)

1. Jahn R., Lang T., Sudhof T.C. Membrane fusion. Cell 2003, 112:519-533.
2. Lang T., Wacker I., Wunderlich I., Rohrbach A., Giese G., Soldati T., Almers W. Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. Biophys. J. 2000, 78:2863-2877.
3. Pendleton A., Koffer A. Effects of latrunculin reveal requirements for the actin cytoskeleton during secretion from mast cells. Cell Motil. Cytoskeleton 2001, 48:37-51.
4. Gasman S., Chasserot-Golaz S., Malacombe M., Way M., Bader M.F. Regulated exocytosis in neuroendocrine cells: a role for subplasmalemmal Cdc42/N-WASP-induced actin filaments. Mol. Biol. Cell 2004, 15:520-531.
5. Gasman S., Chasserot-Golaz S., Popoff M.R., Aunis D., Bader M.F. Involvement of Rho GTPases in calcium-regulated exocytosis from adrenal chromaffin cells. J. Cell Sci. 1999, 112:4763-4771.
6. Daniel S., Noda M., Cerione R.A., Sharp G.W. A link between Cdc42 and syntaxin is involved in mastoparan-stimulated insulin release. Biochemistry 2002, 41:9663-9671.
7. Nevins A.K., Thurmond D.C. A direct interaction between Cdc42 and vesicle-associated membrane protein 2 regulates SNARE-dependent insulin exocytosis. J. Biol. Chem. 2005, 280:1944-1952.
8. Shaner N.C., Campbell R.E., Steinbach P.A., Giepmans B.N., Palmer A.E., Tsien R.Y. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 2004, 22:1567-1572.
9. Nagai T., Ibata K., Park E.S., Kubota M., Mikoshiba K., Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 2002, 20:87-90.
10. Kovacs E.M., Makar R.S., Gertler F.B. Tuba stimulates intracellular N-WASP-dependent actin assembly. J. Cell Sci. 2006, 119:2715-2726.
11. Sato M., Mori Y., Matsui T., Aoki R., Oya M., Yanagihara Y., Fukuda M., Tsuboi T. Role of the polybasic sequence in the Doc2alpha C2B domain in dense-core vesicle exocytosis in PC12 cells. J. Neurochem. 2010, 114:171-181.
12. Kodani A., Kristensen I., Huang L., Sutterlin C. GM130-dependent control of Cdc42 activity at the Golgi regulates centrosome organization. Mol. Biol. Cell 2009, 20:1192-1200.
13. Malacombe M., Ceridono M., Calco V., Chasserot-Golaz S., McPherson P.S., Bader M.F., Gasman S. Intersectin-1L nucleotide exchange factor regulates secretory granule exocytosis by activating Cdc42. EMBO J. 2006, 25:3494-3503.
14. Nakata T., Hirokawa N. Organization of cortical cytoskeleton of cultured chromaffin cells and involvement in secretion as revealed by quick-freeze, deep-etching, and double-label immunoelectron microscopy. J. Neurosci. 1992, 12:2186-2197.
15. Neco P., Giner D., Viniegra S., Borges R., Villarroel A., Gutierrez L.M. New roles of myosin II during vesicle transport and fusion in chromaffin cells. J. Biol. Chem. 2004, 279:27450-27457.
16. Giner D., Lopez I., Villanueva J., Torres V., Viniegra S., Gutierrez L.M. Vesicle movements are governed by the size and dynamics of F-actin cytoskeletal structures in bovine chromaffin cells. Neuroscience 2007, 146:659-669.
17. Luna A., Matas O.B., Martinez-Menarguez J.A., Mato E., Duran J.M., Ballesta J., Way M., Egea G. Regulation of protein transport from the Golgi complex to the endoplasmic reticulum by CDC42 and N-WASP. Mol. Biol. Cell 2002, 13:866-879.
18. Matas O.B., Martinez-Menarguez J.A., Egea G. Association of Cdc42/N-WASP/Arp2/3 signaling pathway with Golgi membranes. Traffic 2004, 5:838-846.