Construction of an expression system for the secretory production of recombinant α-agarase in yeast

Biotechnology Letters

Volumn 34, Issue 6, 2012, Pages 1041-1049

  Seok, Ji Hwan ^a   Kim, Hye Soo ^a   Hatada, Yuji ^b   Nam, Soo Wan ^a   Kim, Yeon Hee ^a  

^a Dong Eui University   (South Korea)

^b JAPAN AGENCY FOR MARINE EARTH SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Agarase; Overexpression system; Pichia pastoris; Saccharomyces cerevisiae; Signal sequence

Indexed keywords

AGARO-OLIGOSACCHARIDES; AGAROSE; EXPRESSION SYSTEM; EXTRACELLULAR; INDUCIBLE EXPRESSION; INDUSTRIAL USE; OVER-EXPRESSION; PICHIA PASTORIS; SIGNAL SEQUENCE;

GENE ENCODING; GENE EXPRESSION; INDUSTRIAL APPLICATIONS;

YEAST;

AGARASE; GLYCOSIDASE; RECOMBINANT PROTEIN;

ARTICLE; ENZYMOLOGY; GAMMAPROTEOBACTERIA; GENE EXPRESSION; GENE VECTOR; GENETICS; PICHIA; PLASMID; SACCHAROMYCES CEREVISIAE; SECRETION;

GAMMAPROTEOBACTERIA; GENE EXPRESSION; GENETIC VECTORS; GLYCOSIDE HYDROLASES; PICHIA; PLASMIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

PICHIA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; THALASSOMONAS;

EID: 84862813531     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-0864-0     Document Type: Article

References (44)

1. Araki CH (1937) Acetylation of agar like substance of Gelidium amansii. J Chem Soc Jpn 58: 1338-1350.
2. Araki T, Hayakawa M, Zhang L, Karita S, Morishita T (1998) Purification and characterization of agarases from a marine bacterium, Vibrio sp. PO-303. J Mar Biotechnol 6: 260-265.
3. Armisén R, Galatas F, Hispanagar SA (2000) Agar. In: Phillips GO, Williams PA (eds) Handbook of hydrocolloids. Woodhead Publishing Ltd, Cambridge, pp 21-40.
4. Bae MC, Kim JO, Shim HS, Byun DS, Cho DM, Kim HR (2003) Purification and characterization of the α-agarase from Pseudomonas sp. AP5333. In: Second international symposium on fisheries sciences and technology for academic exchange between Hokkaido University and Pukyong National University, November 5, Hakodate, Japan.
5. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
6. Do JH (1997) Extraction and purification of agar from Gelidium amansii. J Korean Fish Soc 30: 423-427.
7. Duckworth M, Yaphe W (1971) The structure of agar. I. Fractionation of a complex mixture of polysaccharides. Carbohydr Res 16: 189-197.
8. Emr SD, Schekman R, Flessel MC, Thorner J (1983) An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast. Proc Natl Acad Sci USA 80: 7080-7084.
9. Feng Z, Zhang L, Han X, Zhang Y (2010) Codon optimization of the calf prochymosin gene and its expression in Kluyveromyces lactis. World J Microbiol Biotechnol 26: 895-901.
10. Gietz RD, Schiestl RH, Willems AR, Woods RA (1995) Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11: 355-360.
11. Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein expression. Trends Biotechnol 22: 346-353.
12. Hassairi I, Ben Amar R, Nonus M, Gupta BB (2001) Production and separation of α-agarase from Altermonas agarlyticus GJ1B. Bioresour Technol 79: 47-51.
13. Hatada Y, Ohta Y, Horikoshi K (2006) Hyperproduction and application of alpha-agarase to enzymatic enhancement of antioxidant activity of porphyran. J Agric Food Chem 54: 9895-9900.
14. Holmes WJ, Darby RA, Wilks MD, Smith R, Bill RM (2009) Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime. Microb Cell Factories 8: 35-48.
15. Hosoda A, Sakai M, Kanazawa S (2003) Isolation and characterization of agar-degrading Paenibacillus spp. associated with the rhizosphere of spinach. Biosci Biotechnol Biochem 67: 1048-1055.
16. Ikemura T (1981) Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes. J Mol Biol 146: 1-21.
17. Kato I (2000) Antioxidative and antitumorigenic properties of agarooligosaccharide. Bioindustry 17: 13-19.
18. Kim BJ, Kim HJ, Ha SD, Hwang SH, Byun DS, Lee TH, Kong JY (1999) Purification and characterization of β-agarase from marine bacterium Bacillus cereus ASK202. Biotechnol Lett 21: 1011-1105.
19. Kim MJ, Kim SH, Lee JH, Seo JH, Lee JH, Kim JH, Kim YH, Nam SW (2008) High-level secretory expression of human procarboxypeptidase B by Fed-Batch cultivation of Pichia pastoris and its partial characterization. J Microbiol Biotechnol 18: 1938-1944.
20. Kim MJ, Nam SW, Tamano K, Machida M, Kim SK, Kim YH (2011) Optimization for production of exo-β-1, 3-glucanase (laminarinase) from Aspergillus oryzae in Saccharomyces cerevisiae. KSBB J 26: 427-432.
21. Kirimura K, Masuda N, Iwasaki Y, Nakagawa H, Kobayashi R, Usami S (1999) Purification and characterization of a novel β-agarase from an alkalophilic bacterium, Alteromonas sp. E-1. J Biosci Bioeng 87: 436-441.
22. Kobayashi R, Takimasa M, Suzuki T, Kirimura K, Usami S (1997) Neoagarobiose as a novel moisturizer with whitening effect. Biosci Biotechnol Biochem 61: 162-163.
23. Kohno T, Kitagawa H, Hiraga T (1990) Production of hetero-oligosaccharides. In: Gijutsu, Kenkyu, Kukami (eds) Shokuhin sangyo bioreactor system, Jissen bioreactor. Shokuhin Kagaku Shimbunsa, Tokyo, pp 87-105.
24. Korec E, Korcová J, Palková Z, Vondrejs V, Korínek V, Reinis M, Bichko VV, Hlozánek I (1989) Expression of hepatitis B virus large envelope protein in Escherichia coli and Saccharomyces cerevisiae. Folia Biol (Praha) 35: 315-327.
25. Kwon MJ, Park SL, Kim SK, Nam SW (2002) Overproduction of Bacillus macerans cyclodextrin glucanotransferase in E. coli by coexpression of GroEL/ES chaperone. J Microbiol Biotechnol 12: 1002-1005.
26. Lakshmikanth M, Manohar S, Patnakar J, Vaishampayan P, Shouche Y, Lalitha J (2006) Optimization of culture conditions for the production of extracellular agarases from newly isolated Pseudomonas aeruginosa AG LSL-11. World J Microbiol Biotechnol 22: 531-537.
27. Latchinian-Sadek L, Thomas DY (1993) Expression, purification, and characterization of the yeast KEX1 gene product, a polypeptide precursor processing carboxypeptidase. J Biol Chem 268: 534-540.
28. Lee DG, Park GT, Kim NY, Lee EJ, Jang MK, Shin YG, Park GS, Kim TM, Lee JH, Lee JH, Kim SJ, Lee SH (2006) Cloning, expression, and characterization of a glycoside hydrolase family 50 β-agarase from a marine Agarivorans isolate. Biotechnol Lett 28: 1925-1932.
29. Lee DG, Jang MK, Lee OH, Kim NY, Ju SA, Lee SH (2008) Over-production of a glycoside hydrolase family 50 beta-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product. Biotechnol Lett 30: 911-918.
30. Lim MY, Lee JW, Lee JH, Kim YH, Seo JH, Nam SW (2007) Secretory overexpression of Clostridium endoglucanase A in Saccharomyces cerevisiae using GAL10 promoter and exoinulinase signal sequence. Korean J Life Sci 17: 1248-1254.
31. Marlatt NM, Spratt DE, Shaw GS (2010) Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins. Protein Expr Purif 73: 58-64.
32. Ohta Y, Nogi Y, Miyazaki M, Li Z, Hatada Y, Ito S, Horikoshi K (2004) Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from the novel marine isolate, JAMB-A94. Biosci Biotechnol Biochem 68: 1073-1081.
33. Ohta Y, Hatada Y, Miyazaki M, Nogi Y, Ito S, Horikoshi K (2005) Purification and characterization of a Novel α-agarase from a Thalassomonas sp. Curr Microbiol 50: 212-216.
34. Potin P, Richard C, Rochas C, Kloareg B (1993) Purification and characterization of the α-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B. Eur J Biochem 214: 599-607.
35. Routledge SJ, Hewitt CJ, Bora N, Bill RM (2011) Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield. Microb Cell Factories 10: 17-27.
36. Seok JH, Park HG, Lee SH, Nam SW, Jeon SJ, Kim JH, Kim YH (2010) High-level secretory expression of recombinant β-agarase from Zobellia galactanivorans in Pichia pastoris. Korean J Microbiol Biotechnol 38: 40-45.
37. Shi C, Lu X, Ma C, Ma Y, Fu X, Yu W (2008) Enhancing the thermostability of a novel beta-agarase AgaB through directed evolution. Appl Biochem Biotechnol 151: 51-59.
38. Shin EJ, Lee JW, Kim JH, Jeon SJ, Kim YH, Nam SW (2010) Overexpression, purification, and characterization of β-subunit group II chaperonin from hyperthermophilic Aeropyrum pernix K1. J Microbiol Biotechnol 20: 542-549.
39. Sugano Y, Terada I, Arita M, Noma M (1993) Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107. Appl Environ Microbiol 59: 1549-1554.
40. Villalobos A, Ness JE, Gustafsson C, Minshull J, Govindarajan S (2006) Gene designer: a synthetic biology tool for constructing artificial DNA segments. BMC Bioinformatics 7: 285.
41. Wang J, Jiang X, Mou H, Guan H (2004) Anti-oxidation of agar oligosaccharides produced by agarase from a marine bacterium. J Appl Phycol 16: 333-340.
42. Wang X, Li X, Zhang Z, Shen X, Zhong F (2010) Codon optimization enhances secretory expression of Pseudomonas aeruginosa exotoxin A in E. coli. Protein Expr Purif 72: 101-106.
43. Winston F, Dollard C, Ricupero-Hovasse SL (1995) Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11: 53-55.
44. Zhou Z, Schnake P, Xiao L, Lal AA (2004) Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization. Protein Expr Purif 34: 87-94.