Enhancing the thermostability of a novel β-agarase AgaB through directed evolution

Applied Biochemistry and Biotechnology

Volumn 151, Issue 1, 2008, Pages 51-59

  Shi, Chao ^a,b   Lu, Xinzhi ^a   Ma, Cuiping ^a,b   Ma, Yiming ^a   Fu, Xiaoyan ^a   Yu, Wengong ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Agarase; Clear zone screening; Directed evolution; DNA shuffling; Thermostability

Indexed keywords

AGARASE; CLEAR ZONE SCREENING; DIRECTED EVOLUTION; DNA SHUFFLING; THERMOSTABILITY;

ALGAE; CLUSTER ANALYSIS; DICHROISM; ENZYMES; GENES; HYDROPHOBICITY; MELTING POINT; NUCLEIC ACIDS; ORGANIC ACIDS; POLYSACCHARIDES;

DNA;

AGAB ENZYME; AGAR; GLYCOSIDASE; UNCLASSIFIED DRUG; AGARASE;

ARTICLE; CIRCULAR DICHROISM; DNA SHUFFLING; ENZYME METABOLISM; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN FOLDING; THERMOSTABILITY; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; GENETICS; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING; TEMPERATURE;

DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; GLYCOSIDE HYDROLASES; PROTEIN ENGINEERING; TEMPERATURE;

EID: 53749107844     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-008-8169-4     Document Type: Article

References (17)

1. Percival, E., & McDowell, R. H. (1967). Chemistry and enzymoloyy of marine algal polysaccharides pp. 88-98, 127-137. London: Academic.
2. M. Duckworth J. R. Turvey 1969 Biochemical Journal 113 693 697
3. J. Allouch M. Jam W. Helbert 2003 Journal of Biological Chemistry 278 47171 47180
4. Y. Ohta Y. Hatada Y. Nogi 2004 Applied Microbiology and Biotechnology 64 505 514
5. D. C. Schroeder M. A. Jaffer V. E. Coyne 2003 Microbiology 149 2919 2929
6. Y. Sugano T. Matsumoto H. Kodama 1993 Applied and Environmental Microbiology 59 3750 3756
7. R. Kobayashi M. Takisada T. Suzuki 1997 Bioscience, Biotechnology, and Biochemistry 61 162 163
8. Y. Yoshizawa A. Ametani J. Tsunehiro 1995 Bioscience, Biotechnology, and Biochemistry 59 1933 1937
9. X. Zhao C. Xue Q. Xu 2002 Journal of Fish Science China 9 280 282
10. T. Araki M. Hayakawa Z. Lu 1998 Journal of Marine Biotechnology 6 260 265
11. J. K. Yano T. L. Poulos 2003 Current Opinion in Biotechnology 14 360 365
12. C. P. Ma X. Z. Lu C. Shi 2007 Journal of Biological Chemistry 282 6 3747 3754
13. G. L. Miller 1959 Analytical Chemistry 31 426 428
14. O. A. Bateman R. Sarra S. T. van Genesen 2003 Experimental Eye Research 77 409 422
15. B. K. Shoichet W. A. Baase R. Kuroki 1995 Proceedings of the National Academy of Sciences of the United States of America 92 452 456
16. I. Callebaut G. Labesse P. Durand 1997 Cellular and Molecular Life Sciences 53 621 645
17. L. Lemesle-Varloot B. Henrissat C. Gaboriaud 1990 Biochimie 72 555 574