Isolation and characterization of Pseudomonas brassicacearum J12 as an antagonist against Ralstonia solanacearum and identification of its antimicrobial components

Microbiological Research

Volumn 167, Issue 7, 2012, Pages 388-394

  Zhou, Tiantian ^a   Chen, Da ^a   Li, Chunyu ^a   Sun, Qian ^a   Li, Lingzhi ^a   Liu, Fang ^a   Shen, Qirong ^a   Shen, Biao ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

2,4 DAPG; Biocontrol; PhlACBD gene cluster; Pseudomonas brassicacearum; Ralstonia solanacearum

Indexed keywords

16S RRNA GENE SEQUENCE; 2 ,4-DIACETYLPHLOROGLUCINOL; 2,4-DAPG; ANTAGONISTIC ACTIVITY; ANTIMICROBIAL COMPOUNDS; BACTERIAL STRAINS; CARBON AND NITROGEN; GENE CLUSTERS; GREENHOUSE EXPERIMENTS; HPLC-ESI MS; HYDROGEN CYANIDE; ISOLATION AND CHARACTERIZATION; PHYTOPATHOGENIC BACTERIA; PSEUDOMONAS BRASSICACEARUM; RALSTONIA SOLANACEARUM; RHIZOSPHERE SOILS; SIDEROPHORES; TOMATO PLANTS; TRYPTONE;

BIOCONTROL; ESCHERICHIA COLI; FRUITS; GENES; GLUCOSE; RNA; SOILS;

BACTERIA;

2,4 DIACETYLPHLOROGLUCINOL; 2,4-DIACETYLPHLOROGLUCINOL; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; DRUG DERIVATIVE; PHLOROGLUCINOL;

ANTAGONISM; ANTIMICROBIAL ACTIVITY; BACTERIAL DISEASE; BACTERIUM; BIOLOGICAL CONTROL; BIOTECHNOLOGY; DISEASE CONTROL; ENZYME ACTIVITY; GREENHOUSE ECOSYSTEM; GROWTH REGULATOR; INHIBITION; ORGANIC COMPOUND; PATHOGEN; RHIZOSPHERE; VEGETABLE;

ANTIBIOSIS; ARTICLE; BIOLOGICAL PEST CONTROL; CHEMISTRY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; PHYSIOLOGY; PLANT DISEASE; PSEUDOMONAS; RALSTONIA SOLANACEARUM; TOMATO;

ANTI-BACTERIAL AGENTS; ANTIBIOSIS; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; LYCOPERSICON ESCULENTUM; MOLECULAR SEQUENCE DATA; PEST CONTROL, BIOLOGICAL; PHLOROGLUCINOL; PHYLOGENY; PLANT DISEASES; PSEUDOMONAS; RALSTONIA SOLANACEARUM; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LYCOPERSICON ESCULENTUM; PSEUDOMONAS; PSEUDOMONAS BRASSICACEARUM; RALSTONIA SOLANACEARUM;

EID: 84862789334     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2012.01.003     Document Type: Article

References (59)

1. Amico E.D., Cavalca L., Andreoni V. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal resistant, potentially plant growth-promoting bacteria. FEMS Microbiol Ecol 2005, 52:153-162.
2. Alexander D.B., Zuberer D.A. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Fertil Soils 1991, 12:39-45.
3. Bakker A.W., Schippers B. Microbial cyanide production in therhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biol Biochem 1987, 19:451-457.
4. Bordoloi G., Kumari B., Guha A., Bordoloi M.J., Roy M.K., Bora T.C. Isolation and structural elucidation of a new antifungal and antibacterial antibiotic produced by Streptomyces sp. 201. Biotechnol Biochem 2001, 65:1856-1858.
5. Broadbent D., Mabelis R.P., Spenser H. C-Acetyphloroglucinols from Pseudomonas fluorescens. Phytochemistry 1976, 15:1785.
6. Bowden K., Broadbent J.L., Ross W.J. Some simple anthelmintics. Br J Pharmacol 1965, 24:714-724.
7. Chin-A-Woeng T.F.C., Bloemberg G.V., Lugtenberg B.J.J. Phenazines and their role in biocontrol by Pseudomonas bacteria. New Phytol 2003, 157:503-523.
8. Clough S., Flavier A., Schell M., Denny T. Differential expression of virulence genes and motility in Ralstonia (Pseudomonas) solanacearum during exponential growth. Appl Environ Microbiol 1997, 63:844-850.
9. Defago G., Haas D. Pseudomonads as antagonists of soil borne plant pathogens: mode of action and genetic analysis. Soil Biochem 1990, 6:249-291.
10. De Souza J.T., Arnould C., Deulvot C., Lemanceau P., Gianinazzi-Pearson V., Raaijmakers J.M. Effect of 2, 4-diacetylphloroglucinol on pythium: cellular responses and variation in sensitivity among propagules and species. Phytopathology 2003, 93:966-975.
11. Duffy B.K., Défago G. Zinc improves biocontrol of Fusarium crown and root rot of tomato by Pseudomonas fluorescens and represses the production of pathogen metabolites inhibitory to bacterial antibiotic biosynthesis. Phytopathology 1997, 87:1250-1257.
12. Eden P.A., Schmidt T.M., Blakemore R.P., Pace N.R. Phylogenetic analysis of Aquaspirillum magnetotacticum using polymerase chain reaction-amplified 16S rRNA specific DNA. Int J Syst Bacteriol 1991, 41:324-325.
13. Fravel D.R. Role of antibiosis in the biocontrol of plant diseases. Annu Rev Phytopathol 1988, 26:75-91.
14. Garagulya A.D., Kiprianova E.A., Boiko O.I. Antibiotic effect of bacteria from the genus Pseudomonas on phytopathogenic fungi. Microbiol Zh (Kiev) 1974, 36:197-202.
15. Gleeson O., O'Gara F., Morrissey J.P. The Pseudomonas fluorescens secondary metabolite 2,4 diacetylphloroglucinol impairs mitochondrial function in Saccharomyces cerevisiae. Anton Van Leeuw 2010, 97:261-327. 3.
16. Gurusiddaiah S., Weller D.M., Sarkar A., Cook R.J. Characterization of an antibiotic produced by a strain of Pseudomonas fluorescens inhibitory to Gaeumannomyces graminis var. tritici and Pythium spp. Antimicrob Agents Chemother 1986, 29:488-499.
17. Haas D., Defago G. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat Rev Microbiol 2005, 3:307-319.
18. Hayward H.C. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol 1991, 29:65-87.
19. Howie W.J., Suslow T.V. Role of antibiotic biosynthesis in the inhibition of Pythium ultimum in the cotton spermosphere and rhizosphere by Pseudomonas fluorescens. Mol Plant Microbe Interact 1991, 4:393-399.
20. Howell C.R., Stipanovic R.D. Control of Rhizoctonia solani on cotton seedlings with Pseudomonas fluorescens and with an antibiotic produced by the bacterium. Phytopathology 1979, 69:480-482.
21. Howell C.R., Stipanovic R.D. Suppression of Pythium ultimum-induced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 1980, 70:712-715.
22. Huang J., Xu Y., Zhang H., Li Y., Huang X., Ren B., et al. Temperature-dependent expression of phzM and its regulatory genes lasI and ptsP in rhizosphere isolate Pseudomonas sp. strain M185. Appl Environ Microbiol 2009, 75:6568-6580.
23. Hu H.B., Xu Y.Q., Cheng F., Zhang X.H., Hur B. Isolation and characterization of a new Pseudomonas strain produced both phenazine 1-carboxylic acid and pyoluteorin. J Microbiol Biotechnol 2005, 15:86-90.
24. Jousset A., Lara E., Wall L.G., Valverde C. Secondary metabolites help biocontrol strain Pseudomonas fluorescens CHA0 to escape protozoan grazing. Appl Environ Microbiol 2006, 72:7083-7090.
25. Raaijmakers J.M., Bonsall Robert F., Weller D.M. Effect of Population Density of Pseudomonas fluorescens on Production of 2,4-Diacetylphloroglucinol in the Rhizosphere of Wheat. Appl Environ Microbiol 1999, 89:470-475.
26. Raaijmakers J.M., Vlami M., de Souza J.T. Antibiotic production by bacterial biocontrol agents. Anton Van Leeuw 2002, 81:537-547.
27. Katar'yan B.T., Torgashova G.G. Spectrum and activity of the herbicidal effect of 2,4-diacetylphloroglucinol. Dokl Akad Nauk Arm SSR 1976, 63:109-112.
28. Keel C., Wirthner P., Oberhansli T., Voisard C., Burger U., Haas D., et al. Pseodomonas as antagonists of plant pathogens in the rhizosphere, role of the antibiotic 2,4-diacetylphloroglucinols in the suppression of black root rot of tobacco. Symbiosis 1990, 9:327-341.
29. King E.O., Ward M.K., Raney D.E. Two simple media for demonstration of pyocyanin and fluorescein. J Lab Clin Med 1954, 44:301-307.
30. Kloepper J.W., Leong J., Teintze M., Schroth N.M. Enhanced plant growth by siderophores produced by plant growth promoting rhizobacteria. Nature (London) 1980, 286:885-886.
31. Lam B.S., Strobel G.A., Harrison L.A., Lam S.T. Transposon mutagenesis and tagging of fluorescent Pseudomonas: antimycotic production is necessary for control of Dutch elm disease. Proc Nat Acad Sci (USA) 1987, 84:6447-6451.
32. Leisinger T., Margraff R. Secondary metabolites of fluorescent pseudomonas. Microbiol Rev 1979, 43:422-442.
33. Leong J. Siderophores: their biochemistry and possible role in biocontrol of plant pathogens. Annu Rev Phytopathol 1986, 24:187-209.
34. Mavrodi D.V., Bonsall R.F., Delaney S.M., Soule M.J., Phillips G., Thomashow L.S. Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1. J Bacteriol 2001, 183:6454-6465.
35. Mavrodi O.V., Gardener B.B.M., Mavrodi D.V., Bonsall R.F., Weller D.M., Thomashow L.S. Genetic diversity of phlD from 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. Phytopathology 2001, 91:35-43.
36. Morrissey J.P., Walsh U.F., O'Donnell A., Moenne-Loccoz Y., O'Gara F. Exploitation of genetically modified inoculants for industrial ecology applications. Anton Van Leeuw 2002, 81:599-606.
37. Neiland J.B., Leong S.A. Siderophores in relation to plant disease. Annu Rev Plant Physiol 1986, 37:187-208.
38. O'Sullivan D.J., O'Gara F. Traits of Fluorescent Pseudomonas spp. involved in suppression of plant pathogens. Microbiol Rev 1992, 56:662-672.
39. Patton T., Barrett J., Brennan J., Moran N. Use of aspectrophotometric bioassay for determination of microbial sensitivity to manuka honey. J Microbiol Methods 2006, 64:84-95.
40. Picard C., Bosco M. Heterozygosis drives maize hybrids to select elite 2,4-diacethylphloroglucinol-producing Pseudomonas strains among resident soil populations. FEMS Microbiol Ecol 2006, 58:193-204.
41. Pikovskaya R.I. Mobilization of phosphorous in soil in connection with vital activity of some microbial species. Mikrobiologiya 1948, 17:363-370.
42. Raaijmakers J., Weller D.M., Thomashow L.S. Frequency of antibiotic producing Pseudomonas spp. in natural environments. Appl Environ Microbiol 1997, 63:881-887.
43. Rajkumar M., Lee W.H., Lee K.J. Screening of bacterial antagonists for biological control of Phytophthora blight of pepper. J Basic Microbiol 2005, 45:55-63.
44. Ramette A., Frapolli M., Defago G., Monenne Y. Phylogeny of HCN synthase-encoding hcnBC genes in biocontrol fluorescent pseudomonas and its relationship with host plant species and HCN synthesis ability. Mol Plant Microbe Interact 2003, 16:525-535.
45. Reddi T.K., Borovkov A.V. Antibiotic properties of 2,4-diacetylphloroglucinol produced by Pseudomonas fluorescens strain 26-0. Antibiotiki (Moscow) 1970, 15:19-21.
46. Rezzonico F., Zala M., Keel C., Duffy B., Moenne-Loccoz Y., Defago G. Is the ability of biocontrol fluorescent pseudomonads to produce the antifungal metabolite 2,4-diacetylphloroglucinol really synonymous with higher plant protection?. New Phytol 2007, 173:861-872.
47. Saddiqui I.A., Shaukat S.S. Liquid culture carbon, nitrogen and inorganic phosphate source regulate nematicidal activity by fluorescent pseudomonads in vitro. Lett Appl Microbiol 2004, 38:185-190.
48. Saile E., McGarvey J., Schell M., Denny T. Role of extracellular polysaccharide and endoglucanase in root invasion and colonization of tomato plants by Ralstonia solanacearum. Phytopathology 1997, 87:1264-1271.
49. Sakthivel N., Gnanamanicham S.S. Evaluation of Pseudomonas fluorescens for suppression of sheath rot disease and for enhancement of grain yields in rice (Oryza sativa L.). Appl Environ Microbiol 1987, 53:2056-2059.
50. Shanahan P., O'Sullivan D.J., Simpson P., Glennon J.D., O'Gara F. Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production. Appl Environ Microbiol 1992, 58:353-358.
51. Smibert R.M., Krieg N.R. Phenotypic characterization. Methods for general and molecular bacteriology 1994, 607-654. American Society of Microbiology, Washington, DC. P. Gerhardt, R.G.E. Murray, W.A. Wood, N.R. Krieg (Eds.).
52. Soliveri J., Arias M.E., Laborda E. PA5 and Pa7 pantane and heptane macrolide antibiotics produced by a new isolate of Streptoverticillium from Spanish soil. Appl Microbiol Biotechnol 1987, 25:366-371.
53. Stainier R.Y., Palleroni N.J., Doudoroff M. The aerobic Pseudomonads: a taxonomic study. J Gen Microbiol 1966, 41:159-271.
54. Tada M., Takakuwa T., Nagai M., Antiviral Takao Y. antimicrobial activity of 2,4-diacetylphloroglucinols, 2-acylcyclohexane-1, 3-diones and 2-carboxymidocyclohexane-1,3-diones. Agric Biol Chem 1990, 54:3061-3063.
55. Thomashow L.S., Weller D.M. Current concepts in the use of introduced bacteria for biological disease control: mechanisms and antifungal metabolites. Plant microbe interactions 1995, 187-235. Chapman and Hall, New York. G. Stacey, N. Keen (Eds.).
56. Wei H.L., Zhang L.Q. Quorum-sensing system influences root colonization and biological control ability in Pseudomonas fluorescens 2P24. Anton Van Leeuw 2006, 89:267-280.
57. Weller D.M. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 1988, 26:379-407.
58. Xu G.W., Gross D.C. Selection of fluorescent pseudomonads antagonistic to Erwinia cartovora and suppressive of potato seed piece decay. Phytopathology 1986, 76:414-422.
59. Zhou HY. The genetic regulation of biosynthesis 2,4-diacetylphloroglucinol in Pseudomonas fluorescence 2P24, PhD thesis. China Agricultural University; 2005.