Cultivation at 6-10°C is an effective strategy to overcome the insolubility of recombinant proteins in Escherichia coli

Protein Expression and Purification

Volumn 82, Issue 2, 2012, Pages 297-301

  Song, Jung Min ^a   An, Young Jun ^a   Kang, Mee Hye ^a   Lee, Youn Ho ^a   Cha, Sun Shin ^a  

^a Korea Research Institute of Ships and Ocean Engineering   (South Korea)

Author keywords

E. coli; Extremely low temperature; Protein expression

Indexed keywords

BACTERIAL PROTEIN; CELLULASE; INSECT PROTEIN; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; COLD; CULTURE TECHNIQUE; ESCHERICHIA COLI; GROWTH, DEVELOPMENT AND AGING; METABOLISM; SOLUBILITY;

BACTERIAL PROTEINS; CELLULASE; COLD TEMPERATURE; CULTURE TECHNIQUES; ESCHERICHIA COLI; INSECT PROTEINS; LIPASE; RECOMBINANT PROTEINS; SOLUBILITY;

CRYPTOPYGUS; ESCHERICHIA COLI;

EID: 84862785163     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.01.020     Document Type: Article

References (20)

1. H.P. Sørensen, and K.K. Mortensen Advanced genetic strategies for recombinant protein expression in Escherichia coli J. Biotechnol. 115 2005 113 128
2. R.J. Gouka, P.J. Punt, and C. Van den Hondel Efficient production of secreted proteins by Aspergillus: progress, limitations and prospects Appl. Microbiol. Biotechnol. 47 1997 1 11
3. G.E. Smith, M.D. Summers, and M.J. Fraser Production of human beta interferon in insect cells infected with a baculovirus expression vector Mol. Cell. Biol. 3 1983 2156
4. G. Gellissen Production of recombinant proteins: novel microbial and eukaryotic expression systems 2005 Wiley-VCH
5. F. Baneyx Recombinant protein expression in Escherichia coli Curr. Opin. Biotechnol. 10 1999 411 421
6. C.H. Schein, Production of soluble recombinant proteins in bacteria. Bio/technology(New York, N. Y. 1983) 7 (1989) 1141-1149.
7. G. Feller, O. Le Bussy, and C. Gerday Expression of psychrophilic genes in mesophilic hosts: assessment of the folding state of a recombinant α-amylase Appl. Environ. Microbiol. 64 1998 1163 1165
8. F. Baneyx, and M. Mujacic Recombinant protein folding and misfolding in Escherichia coli Nat. Biotechnol. 22 2004 1399 1408
9. M.K. Shaw, and J.L. Ingraham Synthesis of macromolecules by Escherichia coli near the minimal temperature for growth J. Bacteriol. 94 1967 157 164
10. R.J. Broeze, C.J. Solomon, and D.H. Pope Effects of low temperature on in vivo and in vitro protein synthesis in Escherichia coli and Pseudomonas fluorescens J. Bacteriol. 134 1978 861 874
11. M.K. Shaw, A.G. Marr, and J.L. Ingraham Determination of the minimal temperature for growth of Escherichia coli J. Bacteriol. 105 1971 683 684
12. M. Ferrer, T.N. Chernikova, M.M. Yakimov, P.N. Golyshin, and K.N. Timmis Chaperonins govern growth of Escherichia coli at low temperatures Nat. Biotechnol. 21 2003 1266 1267
13. R.E. Joseph, and A.H. Andreotti Bacterial expression and purification of Interleukin-2 Tyrosine kinase: single step separation of the chaperonin impurity Prot. Expr. Puri. 60 2008 194 197
14. T. Annamalai, N. Dani, B. Cheng, and Y.C. Tse-Dinh Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol BMC Biochem. 10 2009 18 26
15. A. Helaly, T. Jiang, and D.R. Bell Expression of the C. elegans aryl hydrocarbon receptor ligand-binding domain Toxicology 253 2008 25-25
16. P.H. Bessette, F. Aslund, J. Beckwith, and G. Georgiou Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm Proc. Natl. Acad. Sci. USA 1999 13703 13708
17. M.M. Bradford A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
18. H. Stalbrand, M. Siika-aho, M. Tenkanen, and L. Viikari Purification and characterization of two β-mananase from Trichoderma reesei J. Biotechnol. 29 1993 229 242
19. F.W. Studier, and B.A. Moffatt Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes J. Mol. Biol. 189 1986 113 130
20. M. Strocchi, M. Ferrer, K.N. Timmis, and P.N. Golyshin Low temperature-induced systems failure in Escherichia coli: insights from rescue by cold-adapted chaperones. Proteomics 6 2006 193 206