Comparison of two expression systems using COS7 cells and yeast cells for expression of heart/muscle-type carnitine palmitoyltransferase 1

Protein Expression and Purification

Volumn 82, Issue 1, 2012, Pages 192-196

  Hada, Takuya ^a   Kato, Yumiko ^a   Obana, Eriko ^a   Yamamoto, Atsushi ^a   Yamazaki, Naoshi ^a   Hashimoto, Mitsuru ^b   Yamamoto, Takenori ^a   Shinohara, Yasuo ^a  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b MATSUYAMA UNIVERSITY   (Japan)

Author keywords

Carnitine palmitoyltransferase (CPT1); Expression system; Mitochondria

Indexed keywords

CARNITINE PALMITOYLTRANSFERASE; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CELL STRAIN COS1; CERCOPITHECUS; COMPARATIVE STUDY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; HEART MUSCLE; METABOLISM; MITOCHONDRION; RABBIT; RAT; SACCHAROMYCES CEREVISIAE;

ANIMALS; CARNITINE O-PALMITOYLTRANSFERASE; CERCOPITHECUS AETHIOPS; COS CELLS; GENE EXPRESSION; MITOCHONDRIA; MYOCARDIUM; RABBITS; RATS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

RATTUS;

EID: 84862783900     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.01.006     Document Type: Article

References (20)

1. J.D. McGarry, and N.F. Brown The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis Eur. J. Biochem. 244 1997 1 14 (Pubitemid 27079689)
2. R.R. Ramsay, R.D. Gandour, and F.R. van der Leij Molecular enzymology of carnitine transfer and transport Biochim. Biophys. Acta 1546 2001 21 43 (Pubitemid 32217800)
3. D.W. Foster The role of the carnitine system in human metabolism Ann. NY Acad. Sci. 1033 2004 1 16 (Pubitemid 39656734)
4. V.A. Zammit Carnitine palmitoyltransferase 1: central to cell function UBMB Life 60 2008 347 354
5. V. Esser, C.H. Britton, B.C. Weis, D.W. Foster, and J.D. McGarry Cloning, sequencing, and expression of a cDNA encoding rat liver carnitine palmitoyltransferase I. Direct evidence that a single polypeptide is involved in inhibitor interaction and catalytic function J. Biol. Chem. 268 1993 5817 5822 (Pubitemid 23090912)
6. N. Yamazaki, Y. Shinohara, A. Shima, and H. Terada High expression of a novel carnitine palmitoyltransferase I like protein in rat brown adipose tissue and heart: isolation and characterization of its cDNA clone FEBS Lett. 363 1995 41 45
7. N. Price, F. van der Leij, V. Jackson, C. Corstorphine, R. Thomson, A. Sorensen, and V. Zammit A novel brain-expressed protein related to carnitine palmitoyltransferase I Genomics 80 2002 433 442 (Pubitemid 35300742)
8. K.F. Woeltje, M. Kuwajima, D.W. Foster, and J.D. McGarry Characterization of the mitochondrial carnitine palmitoyltransferase enzyme system. II. Use of detergents and antibodies J. Biol. Chem. 262 1987 9822 9827
9. N.F. Brown, V. Esser, D.W. Foster, and J.D. McGarry Expression of a cDNA for rat liver carnitine palmitoyltransferase I in yeast establishes that catalytic activity and malonyl-CoA sensitivity reside in a single polypeptide J. Biol. Chem. 269 1994 26438 26442 (Pubitemid 24328467)
10. V. Esser, N.F. Brown, A.T. Cowan, D.W. Foster, and J.D. McGarry Expression of a cDNA isolated from rat brown adipose tissue and heart identifies the product as the muscle isoform of carnitine palmitoyltransferase I (M-CPT I). M-CPT I is the predominant CPT I isoform expressed in both white (epididymal) and brown adipocytes J. Biol. Chem. 271 1996 6972 6977
11. S.T. Swanson, D.W. Foster, J.D. McGarry, and N.F. Brown Roles of the N- and C-terminal domains of carnitine palmitoyltransferase I isoforms in malonyl-CoA sensitivity of the enzymes: insights from expression of chimaeric proteins and mutation of conserved histidine residues Biochem. J. 335 1998 513 519
12. Y. de Vries, D.N. Arvidson, H.R. Waterham, J.M. Cregg, and G. Woldegiorgis Functional characterization of mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris Biochemistry 36 1997 5285 5292 (Pubitemid 27199746)
13. J. Shi, H. Zhu, D.N. Arvidson, and G. Woldegiorgis The first 28 N-terminal amino acid residues of human heart muscle carnitine palmitoyltransferase I are essential for malonyl CoA sensitivity and high-affinity binding Biochemistry 39 2000 712 717 (Pubitemid 30065040)
14. T. Matsuo, A. Yamamoto, T. Yamamoto, K. Otsuki, N. Yamazaki, M. Kataoka, H. Terada, and Y. Shinohara Replacement of C305 in heart/muscle-type isozyme of human carnitine palmitoyltransferase I with aspartic acid and other amino acids Biochem. Genet. 48 2010 193 201
15. H. Liu, G. Zheng, M. Treber, J. Dai, and G. Woldegiorgis Cysteine-scanning mutagenesis of muscle carnitine palmitoyltransferase I reveals a single cysteine residue (Cys-305) is important for catalysis J. Biol. Chem. 280 2005 4524 4531 (Pubitemid 40288620)
16. H. Niwa, K. Yamamura, and J. Miyazaki Efficient selection for high-expression transfectants with a novel eukaryotic vector Gene 108 1991 193 199
17. Y. Shinohara, M.R. Almofti, T. Yamamoto, T. Ishida, F. Kita, H. Kanzaki, M. Ohnishi, K. Yamashita, S. Shimizu, and H. Terada Permeability transition-independent release of mitochondrial cytochrome c induced by valinomycin Eur. J. Biochem. 269 2002 5224 5230 (Pubitemid 35340829)
18. N. Yamazaki, T. Matsuo, M. Kurata, M. Suzuki, T. Fujiwaki, S. Yamaguchi, H. Terada, and Y. Shinohara Substitutions of three amino acids in human heart/muscle type carnitine palmitoyltransferase I caused by single nucleotide polymorphisms Biochem. Genet. 46 2008 54 63
19. M. Hashimoto, Y. Shinohara, E. Majima, T. Hatanaka, N. Yamazaki, and H. Terada Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers Biochim. Biophys. Acta 1409 1999 113 124 (Pubitemid 29044061)
20. 2+-induced permeability transition can be observed even in yeast mitochondria under optimized experimental conditions Biochim. Biophys. Acta 1787 2009 1486 1491