Identification of bacterial pathogens in ascitic fluids from patients with suspected spontaneous bacterial peritonitis by use of broad- range PCR (16S PCR) coupled with high-resolution melt analysis

Journal of Clinical Microbiology

Volumn 50, Issue 7, 2012, Pages 2428-2432

  Hardick, Justin ^a   Won, Helen ^a   Jeng, Kevin ^b   Hsieh, Yu Hsiang ^a   Gaydos, Charlotte A ^a   Rothman, Richard E ^a   Yang, Samuel ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; RNA 16S;

ALPHA HEMOLYTIC STREPTOCOCCUS; ARTICLE; ASCITES FLUID; BACTERIAL PERITONITIS; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BETA HEMOLYTIC STREPTOCOCCUS; BROAD RANGE 16S POLYMERASE CHAIN REACTION; COAGULASE NEGATIVE STAPHYLOCOCCUS; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC TEST; ENTEROBACTER AEROGENES; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; HIGH RESOLUTION MELT ANALYSIS; HUMAN; KLEBSIELLA; MAJOR CLINICAL STUDY; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; OUTCOME ASSESSMENT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SENSITIVITY AND SPECIFICITY; SERRATIA MARCESCENS; SPECIES DIFFERENTIATION; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INTERMEDIUS; STREPTOCOCCUS ANGINOSUS; STREPTOCOCCUS CONSTELLATUS;

ASCITIC FLUID; BACTERIA; BACTERIAL INFECTIONS; DNA, BACTERIAL; DNA, RIBOSOMAL; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; PERITONITIS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; TRANSITION TEMPERATURE;

EID: 84862726713     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00345-12     Document Type: Article

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