Characterization of human lung cancer-associated fibroblasts in three-dimensional in vitro co-culture model

Biochemical and Biophysical Research Communications

Volumn 423, Issue 1, 2012, Pages 158-163

  Horie, Masafumi ^a   Saito, Akira ^a   Mikami, Yu ^a   Ohshima, Mitsuhiro ^b   Morishita, Yasuyuki ^a   Nakajima, Jun ^a   Kohyama, Tadashi ^a   Nagase, Takahide ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b OHU UNIVERSITY   (Japan)

Author keywords

SMA; Smooth muscle actin; CAFs; Cancer associated fibroblast; DMEM; ECM; FBS; Gel contraction assay; NFs; NSCLC; Primary culture; Three dimensional co culture

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN GEL; EOSIN; HEMATOXYLIN; VIMENTIN;

ARTICLE; CANCER ASSOCIATED FIBROBLAST; CANCER CELL; CELL INVASION; CELL PROLIFERATION; CELL STRUCTURE; COCULTURE; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; LUNG NON SMALL CELL CANCER; LUNG PARENCHYMA; LUNG RESECTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ACTINS; CELL SEPARATION; COCULTURE TECHNIQUES; FIBROBLASTS; HUMANS; LUNG NEOPLASMS; MYOFIBROBLASTS; STROMAL CELLS; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 84862700312     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.05.104     Document Type: Article

References (35)

1. Siegel R., Naishadham D., Jemal A. Cancer statistics, 2012. CA Cancer J. Clin. 2012, 62:10-29.
2. Haviv I., Polyak K., Qiu W., Hu M., Campbell I. Origin of carcinoma associated fibroblasts. Cell Cycle 2009, 8:589-595.
3. Kalluri R., Zeisberg M. Fibroblasts in cancer. Nat. Rev. Cancer 2006, 6:392-401.
4. Mueller M.M., Fusenig N.E. Friends or foes - bipolar effects of the tumour stroma in cancer. Nat. Rev. Cancer 2004, 4:839-849.
5. östman A., Augsten M. Cancer-associated fibroblasts and tumor growth-bystanders turning into key players. Curr. Opin. Genet. Dev. 2009, 19:67-73.
6. Saito R.A., Micke P., Paulsson J., Augsten M., Peña C., Jönsson P., Botling J., Edlund K., Johansson L., Carlsson P., Jirström K., Miyazono K., östman A. Forkhead box F1 regulates tumor-promoting properties of cancer-associated fibroblasts in lung cancer. Cancer Res. 2010, 70:2644-2654.
7. Gabbiani G., Ryan G.B., Majne G. Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction. Experientia 1971, 27:549-550.
8. Joyce J.A. Therapeutic targeting of the tumor microenvironment. Cancer Cell 2005, 7:513-520.
9. Micke P., östman A. Tumour-stroma interaction: cancer-associated fibroblasts as novel targets in anti-cancer therapy?. Lung Cancer 2004, 45(Suppl 2):S163-S175.
10. Gonda T.A., Varro A., Wang T.C., Tycko B. Molecular biology of cancer-associated fibroblasts: can these cells be targeted in anti-cancer therapy?. Semin. Cell Dev. Biol. 2010, 21:2-10.
11. Navab R., Strumpf D., Bandarchi B., Zhu C.Q., Pintilie M., Ramnarine V.R., Ibrahimov E., Radulovich N., Leung L., Barczyk M., Panchal D., To C., Yun J.J., Der S., Shepherd F.A., Jurisica I., Tsao M.S. Prognostic gene-expression signature of carcinoma-associated fibroblasts in non-small cell lung cancer. Proc. Nat. Acad. Sci. U.S.A. 2011, 108:7160-7165.
12. Sadlonova A., Bowe D.B., Novak Z., Mukherjee S., Duncan V.E., Page G.P., Frost A.R. Identification of molecular distinctions between normal breast-associated fibroblasts and breast cancer-associated fibroblasts. Cancer Microenviron. 2009, 2:9-21.
13. Bauer M., Su G., Casper C., He R., Rehrauer W., Friedl A. Heterogeneity of gene expression in stromal fibroblasts of human breast carcinomas and normal breast. Oncogene 2010, 29:1732-1740.
14. Nakagawa H., Liyanarachchi S., Davuluri R.V., Auer H., Martin E.W., de la Chapelle A., Frankel W.L. Role of cancer-associated stromal fibroblasts in metastatic colon cancer to the liver and their expression profiles. Oncogene 2004, 23:7366-7377.
15. Zhang C., Fu L., Fu J., Hu L., Yang H., Rong T.H., Li Y., Liu H., Fu S.B., Zeng Y.X., Guan X.Y. Fibroblast growth factor receptor 2-positive fibroblasts provide a suitable microenvironment for tumor development and progression in esophageal carcinoma. Clin. Cancer Res. 2009, 15:4017-4027.
16. Anderberg C., Pietras K. On the origin of cancer-associated fibroblasts. Cell Cycle 2009, 8:1461-1462.
17. Sugimoto H., Mundel T.M., Kieran M.W., Kalluri R. Identification of fibroblast heterogeneity in the tumor microenvironment. Cancer Biol. Ther. 2006, 5:1640-1646.
18. Choe M.M., Sporn P.H., Swartz M.A. An in vitro airway wall model of remodeling. Am. J. Physiol. Lung Cell. Mol. Physiol. 2003, 285:L427-L433.
19. Choe M.M., Tomei A.A., Swartz M.A. Physiological 3D tissue model of the airway wall and mucosa. Nat. Protoc. 2006, 1:357-362.
20. Vaughan M.B., Ramirez R.D., Wright W.E., Minna J.D., Shay J.W. A three-dimensional model of differentiation of immortalized human bronchial epithelial cells. Differentiation 2006, 74:141-148.
21. Dolznig H., Rupp C., Puri C., Haslinger C., Schweifer N., Wieser E., Kerjaschki D., Garin-Chesa P. Modeling colon adenocarcinomas in vitro a 3D co-culture system induces cancer-relevant pathways upon tumor cell and stromal fibroblast interaction. Am. J. Pathol. 2011, 179:487-501.
22. Commandeur S., Ho S.H., de Gruijl F.R., Willemze R., Tensen C.P., El Ghalbzouri A. Functional characterization of cancer-associated fibroblasts of human cutaneous squamous cell carcinoma. Exp. Dermatol. 2011, 20:737-742.
23. Pageau S.C., Sazonova O.V., Wong J.Y., Soto A.M., Sonnenschein C. The effect of stromal components on the modulation of the phenotype of human bronchial epithelial cells in 3D culture. Biomaterials 2011, 32:7169-7180.
24. Mio T., Adachi Y., Romberger D.J., Ertl R.F., Rennard S.I. Regulation of fibroblast proliferation in three-dimensional collagen gel matrix. In Vitro Cell. Dev. Biol. Anim. 1996, 32:427-433.
25. Ikebe D., Wang B., Suzuki H., Kato M. Suppression of keratinocyte stratification by a dominant negative JunB mutant without blocking cell proliferation. Genes Cells 2007, 12:197-207.
26. Ohshima M., Yamaguchi Y., Matsumoto N., Micke P., Takenouchi Y., Nishida T., Kato M., Komiyama K., Abiko Y., Ito K., Otsuka K., Kappert K. TGF-β signaling in gingival fibroblast-epithelial interaction. J. Dent. Res. 2010, 89:1315-1321.
27. Olumi A.F., Grossfeld G.D., Hayward S.W., Carroll P.R., Tlsty T.D., Cunha G.R. Carcinoma-associated fibroblasts direct tumor progression of initiated human prostatic epithelium. Cancer Res. 1999, 59:5002-5011.
28. Serini G., Gabbiani G. Mechanisms of myofibroblast activity and phenotypic modulation. Exp. Cell Res. 1999, 250:273-283.
29. Hinz B., Celetta G., Tomasek J.J., Gabbiani G., Chaponnier C. Alpha-smooth muscle actin expression upregulates fibroblast contractile activity. Mol. Biol. Cell 2001, 12:2730-2741.
30. Martin B., Paesmans M., Mascaux C., Berghmans T., Lothaire P., Meert A.P., Lafitte J.J., Sculier J.P. Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis. Br. J. Cancer 2004, 91:2018-2025.
31. Karnoub A.E., Dash A.B., Vo A.P., Sullivan A., Brooks M.W., Bell G.W., Richardson A.L., Polyak K., Tubo R., Weinberg R.A. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Nature 2007, 449:557-563.
32. Mueller L., Goumas F.A., Affeldt M., Sandtner S., Gehling U.M., Brilloff S., Walter J., Karnatz N., Lamszus K., Rogiers X., Broering D.C. Stromal fibroblasts in colorectal liver metastases originate from resident fibroblasts and generate an inflammatory microenvironment. Am. J. Pathol. 2007, 171:1608-1618.
33. Radisky D.C., Kenny P.A., Bissell M.J. Fibrosis and cancer: do myofibroblasts come also from epithelial cells via EMT?. J. Cell. Biochem. 2007, 101:830-839.
34. Direkze N.C., Hodivala-Dilke K., Jeffery R., Hunt T., Poulsom R., Oukrif D., Alison M.R., Wright N.A. Bone marrow contribution to tumor-associated myofibroblasts and fibroblasts. Cancer Res. 2004, 64:8492-8495.
35. Zeisberg E.M., Potenta S., Xie L., Zeisberg M., Kalluri R. Discovery of endothelial to mesenchymal transition as a source for carcinoma-associated fibroblasts. Cancer Res. 2007, 67:10123-10128.