Preparation and characterization of agarose-gelatin blend hydrogels as a cell encapsulation matrix: An in-vitro study

Journal of Macromolecular Science, Part B: Physics

Volumn 51, Issue 8, 2012, Pages 1606-1616

  Imani, Rana ^a,b   Emami, Shahriar Hojjati ^a   Moshtagh, Parisa Rahnama ^a,b   Baheiraei, Nafiseh ^a   Sharifi, Ali Mohammad ^b  

^a AMIRKABIR UNIVERSITY OF TECHNOLOGY   (Iran)

^b TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

agarose; blending; cell therapy; encapsulation; gelatin; hydrogel

Indexed keywords

AGAROSE; BLEND HYDROGELS; CELL ATTACHMENTS; CELL ENCAPSULATIONS; CELL THERAPY; CELL VIABILITY; COMPLEX VISCOSITY; GEL POINT; GELATIN; IMMUNOSUPPRESSANTS; IN-VITRO; MICROCAPSULES; MTT ASSAYS; NONVIRAL; NOVEL MATERIALS; PHOSPHATE BUFFER SOLUTIONS; PHYSIOLOGICAL CONDITION; RHEOLOGICAL BEHAVIORS; SHEAR FORCE; SOL-GEL TRANSITIONS; WEIGHT PERCENTAGES; WEIGHT RATIOS;

BLENDING; BROMINE COMPOUNDS; CELLS; CYTOLOGY; ENCAPSULATION; RHEOLOGY; SOL-GELS;

HYDROGELS;

EID: 84862682800     PISSN: 00222348     EISSN: 1525609X     Source Type: Journal    
DOI: 10.1080/00222348.2012.657110     Document Type: Article

References (22)

1. Murua, A.; Portero, A.; Orive, G.; Hernández, R.M.; Castro, M.; Pedraz, J.L. Cell microencapsulation technology: Towards clinical application. J. Control. Release 2008, 132, 76.
2. Baroli, B. Hydrogels for tissue engineering and delivery of tissue inducing substances. J. Pharm. Sci. 2007, 96, 2197.
3. Ruel-Garíepy, E.; Leroux, J. In situ forming hydrogels-review of temperature sensitive systems. Eur. J. Pharm. Biopharm. 2004, 58, 409.
4. Changez, M.; Burugapalli, K.; Koul, V.; Choudhary, V. The effect of composition of poly(acrylic acid)-gelatin hydrogel on gentamicin sulphate release: In vitro. Biomaterials 2003, 24, 527.
5. Bae, Y.H.; Kim, S.W. Hydrogel delivery systems based on polymer blends, block co-polymers or interpenetrating networks. Adv. Drug Delivery Rev. 1999, 11, 109.
6. Sung, H.W.; Huang, D.M.; Chang, W.H.; Huang, R.N.; Hsu, J.C. Evaluation of gelatin hydrogel crosslinked with various crosslinking agents as bioadhesives: In vitro study. J. Biomed. Mater. Res. 1999, 46, 520.
7. Federovich, N.E.; Alblas, J.; Dewijn, J.R.; Hennink, W.E. Hydrogels as extracellular matrices for skeletal tissue engineering: State-of-The-Art and novel application in organ printing. Tissue Eng. 2007, 13, 1905.
8. Sakai, S.; Kawabata, K.; Ono, T.; Ijima, H.; Kawakami, K. Development of mammalian cellenclosing subsieve size agarose capsules (< 100 μm) for cell therapy. Biomaterials. 2005,26,4786.
9. Gruber, H.E.; Hoelscher, G.L.; Leslie, K.; Ingram, J.A.; Hanley, E.N. Three-dimensional culture of human disc cells within agarose or a collagen sponge: Assessment of proteoglycan production. Biomaterials 2007, 27, 371.
10. Borkenhagen, M.; Clemence, J.F.; Sigrist, H.; Aebischer, P. Three-dimensional extracellular matrix engineering in the nervous system. J. Biomed. Mater. Res. 1998, 40, 392.
11. Batorsky, A.; Liao, J.; Lund, A.W.; Plopper, G.E.; Stegemann, J.P. Encapsulation of adult human mesenchymal stem cells within collagen-Agarose microenvironments. Biotechnol. Bioeng. 2005, 92, 492.
12. Sakai, S.; Hashimoto, I.; Kawakami, K. Agarose-gelatin conjugate for adherent cell-enclosing capsules. Biotechnol. Lett. 2007, 29, 731.
13. Landers, R.; Ubner, H.; Schmelzeisen, U.; Rolf, R.; Ulhaupt, M. Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. Biomaterials 2002, 23, 4437.
14. Verma, V.P.; Kar, S.; Ray, P.R.; Ray, A. Fabrication of agar-gelatin hybrid scaffolds using a novel entrapment method for in-vitro tissue engineering applications. Biotechnol. Bioengin. 2007, 96, 392.
15. Lu, H.F.; Targonsky, E.D.; Wheeler, M.B.; Cheng, Y.L. Thermally induced gelable polymer networks for living cell encapsulation. Biotechnol. Bioengin. 2007, 96, 146.
16. Wang, M.; Li, Y.;Wu, J.; Xu, F.; Zuo, Y.; Jansen, J.A. In vitro and in vivo study to the biocompatibility and biodegradation of hydroxyapatite/ poly(vinyl alcohol)/gelatin composite. J. Biomed. Mater. Res. A 2008, 85, 418.
17. Yin, C.; Chia, S.M.; Quek, C.H.; Yu, H.; Zhuo, R.X.; Leong, K.W.; Mao, H.Q. Microcapsules with improved mechanical stability for hepatocyte culture. Biomaterials 2003, 24, 1771.
18. Zhu, J.H.; Wang, X.W.; Ng, S.; Quek, C.H.; Ho, H.T.; Lao, X.J.; Yu, H. Encapsulating live cells with water-soluble chitosan in physiological conditions. J. Biotecnol. 2005, 117, 355.
19. Jeong, B.; Kim, S.W.; Bae, Y.H. Thermosensitive sol-gel reversible hydrogels. Adv. Drug Delivery Rev. 2002, 54, 37.
20. China Standard, GB/T 16886.5-1997, Biological Evaluation of Medical Devices-Part 5: Tests for Cytotoxicity: In vitro methods, 1997.
21. Santinath Singh, S.; Aswal, V.K.; Bohidar, H.B. Structural studies of agar-gelatin complex coacervates by small angle neutron scattering, rheology and differential scanning calorimetry. Int. J. Biol. Macromol. 2007, 41, 301.
22. Baruch, L.; Machluf, M. Alginate-chitosan complex coacervation for cell encapsulation: Effect on mechanical properties and on long-Term viability. Biopolymers. 2006, 82, 570.