A multiplex immunoassay gives different results than singleplex immunoassays which may bias epidemiologic associations

Clinical Biochemistry

Volumn 45, Issue 10-11, 2012, Pages 848-851

  de Koning, Lawrence ^a,b   Liptak, Cary ^b   Shkreta, Aida ^b   Bradwin, Gary ^b   Hu, Frank B ^a,c   Pradhan, Aruna Das ^c   Rifai, Nader ^b,d   Kellogg, Mark D ^b,d  

^a HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Immunoassay; Inflammation; Multiplex; Sugar sweetened beverage

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 1BETA; INTERLEUKIN 6; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BEVERAGE; COHORT ANALYSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOASSAY; INTERMETHOD COMPARISON; MULTIPLEX IMMUNOASSAY; PRIORITY JOURNAL; SUGAR SWEETENED BEVERAGE;

BEVERAGES; BIAS (EPIDEMIOLOGY); ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOASSAY; INTERFERON-GAMMA; INTERLEUKIN-1BETA; INTERLEUKIN-6; REPRODUCIBILITY OF RESULTS; SWEETENING AGENTS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84862578078     PISSN: 00099120     EISSN: 18732933     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2012.04.006     Document Type: Article

References (11)

1. Dupont N.C., Wang K., Wadhwa P.D., Culhane J.F., Nelson E.L. Validation and comparison of luminex multiplex cytokine analysis kits with elisa: determinations of a panel of nine cytokines in clinical sample culture supernatants. J Reprod Immunol 2005, 66:175-191.
2. Dossus L., Becker S., Achaintre D., Kaaks R., Rinaldi S. Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with Elisa. J Immunol Methods 2009, 350:125-132.
3. Martos-Moreno G.A., Burgos-Ramos E., Canelles S., Argente J., Barrios V. Evaluation of a multiplex assay for adipokine concentrations in obese children. Clin Chem Lab Med 2010, 48:1439-1446.
4. Ray C.A., Bowsher R.R., Smith W.C., Devanarayan V., Willey M.B., Brandt J.T., et al. Development, validation, and implementation of a multiplex immunoassay for the simultaneous determination of five cytokines in human serum. J Pharm Biomed Anal 2005, 36:1037-1044.
5. Ridker P.M., Danielson E., Fonseca F.A., Genest J., Gotto A.M., Kastelein J.J., et al. Rosuvastatin to prevent vascular events in men and women with elevated c-reactive protein. N Engl J Med 2008, 359:2195-2207.
6. Deal A.M., Pate V.W., El Rouby S. Paper cc-014-a sas® macro for deming regression. SouthEast SAS® User Group (SESUG) Conference. Birmingham, AL 2009.
7. de Koning L., Malik V.S., Kellogg M.D., Rimm E.B., Willett W.C., Hu F.B. Sweetened beverage consumption, incident coronary heart disease and biomarkers of risk in men. Circulation 2012, 125(14):1735-1741.
8. Esposito K., Nappo F., Marfella R., Giugliano G., Giugliano F., Ciotola M., et al. Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress. Circulation 2002, 106:2067-2072.
9. Lee J.W., Devanarayan V., Barrett Y.C., Weiner R., Allinson J., Fountain S., et al. Fit-for-purpose method development and validation for successful biomarker measurement. Pharm Res 2006, 23:312-328.
10. Backen A.C., Cummings J., Mitchell C., Jayson G., Ward T.H., Dive C. 'fit-for-purpose' validation of searchlight multiplex elisas of angiogenesis for clinical trial use. J Immunol Methods 2009, 342:106-114.
11. McCullough M.L., Feskanich D., Stampfer M.J., Giovannucci E.L., Rimm E.B., Hu F.B., et al. Diet quality and major chronic disease risk in men and women: moving toward improved dietary guidance. Am J Clin Nutr 2002, 76:1261-1271.