Proteomic Strategy for Identifying Mollusc Shell Proteins Using Mild Chemical Degradation and Trypsin Digestion of Insoluble Organic Shell Matrix: A Pilot Study on Haliotis tuberculata

Marine Biotechnology

Volumn 14, Issue 4, 2012, Pages 446-458

  Bédouet, Laurent ^a   Marie, Arul ^a   Berland, Sophie ^a   Marie, Benjamin ^a,d   Auzoux Bordenave, Stéphanie ^b,c   Marin, Frédéric ^d   Milet, Christian ^a  

^a MUSÉUM NATIONAL D'HISTOIRE NATURELLE   (France)

^b INSTITUT DE RECHERCHE POUR LE DÉVELOPPEMENT   (France)

^c SORBONNE UNIVERSITÉ   (France)

^d UNIVERSITÉ DE BOURGOGNE   (France)

Author keywords

Abalone; Ladder sequencing; Mollusc shell proteomics; Trifluoroacetic acid; Trypsin

Indexed keywords

ABALONE; CHEMICAL DEGRADATION; CHEMICAL DIGESTION; CLEAVAGE SPECIFICITY; DATABASE ANNOTATION; EXPRESSED SEQUENCE TAGS; HALIOTIS; HALIOTIS ASININA; MOLLUSC SHELLS; ORGANIC SHELLS; PILOT STUDIES; PROTEIN SEQUENCES; PROTEOMIC ANALYSIS; PROTEOMICS; SHELL MATRIX PROTEINS; TRIFLUOROACETIC ACIDS; TROPICAL ABALONE; TRYPSIN; TRYPSIN DIGESTION;

ACETIC ACID; AMINO ACIDS; CHEMICAL ANALYSIS; DEGRADATION; MOLECULAR BIOLOGY; PEPTIDES; SHELLFISH; TISSUE; TUBES (COMPONENTS);

SHELLS (STRUCTURES);

PROTEOME; TRYPSIN;

ACETIC ACID; BIODEGRADATION; DATABASE; ENZYME ACTIVITY; FRACTIONATION; IDENTIFICATION METHOD; MATRIX; PEPTIDE; PROTEIN; PROTEOMICS; SNAIL;

ANIMAL; ANIMAL SHELL; ARTICLE; CHEMISTRY; EXTRACELLULAR MATRIX; METABOLISM; METHODOLOGY; MOLLUSC; PILOT STUDY; PROTEOMICS; SOLUBILITY;

ANIMAL SHELLS; ANIMALS; EXTRACELLULAR MATRIX; MOLLUSCA; PILOT PROJECTS; PROTEOME; PROTEOMICS; SOLUBILITY; TRYPSIN;

HALIOTIDAE; HALIOTIS ASININA; HALIOTIS TUBERCULATA; MOLLUSCA;

EID: 84862501755     PISSN: 14362228     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10126-011-9425-0     Document Type: Article

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