Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum

Malaria Journal

Volumn 11, Issue , 2012, Pages

  Hasenkamp, Sandra ^a   Russell, Karen T ^a   Horrocks, Paul ^a  

^a KEELE UNIVERSITY   (United Kingdom)

Author keywords

Electroporation; Luciferase; Malaria; Transfection efficiency

Indexed keywords

DNA; LUCIFERASE;

ARTICLE; CONTROLLED STUDY; DNA TRANSFECTION; ELECTROPORATION; ERYTHROCYTE; GENE EXPRESSION; GENE SEQUENCE; NONHUMAN; PARASITE DEVELOPMENT; PLASMODIUM FALCIPARUM; QUANTITATIVE ANALYSIS; REPORTER GENE; COMPARATIVE STUDY; EVALUATION; GENETIC TRANSFECTION; GENETICS; METHODOLOGY; MOLECULAR BIOLOGY; PARASITOLOGY;

ELECTROPORATION; GENES, REPORTER; LUCIFERASES; MOLECULAR BIOLOGY; PARASITOLOGY; PLASMODIUM FALCIPARUM; TRANSFECTION;

EID: 84862499525     PISSN: None     EISSN: 14752875     Source Type: Journal    
DOI: 10.1186/1475-2875-11-210     Document Type: Article

References (15)

1. Functional genetics in Apicomplexa: potentials and limits. Limenitakis J, Soldati-Favre D, FEBS Lett 2011 585 1579 10.1016/j.febslet.2011.05.002 21557944
2. AdVancements in transfection technologies for Plasmodium. Balu B, Adamas JH, Int J Parasitol 2007 26 1
3. Manipulating the Plasmodium genome. Carvalho TG, Ménard R, Curr Issues Mol Biol 2005 7 39 15580779
4. Comparison of Plasmdodium falciparum transfection methods. Skinner Adams T, Lawrie PM, Hawthorne PL, Gardiner DL, Trenholme KR, Malar J 2003 2 19 10.1186/1475-2875-2-19 12869208
5. Transfection of Plasmodium falciparum within human red blood cells. Wu Y, Sifri CD, Lei HH, Su XZ, Wellems TE, Proc Natl Acad Sci USA 1995 92 973 10.1073/pnas.92.4.973 7862676
6. Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Fidock DA, Wellems TE, Proc Natl Acad Sci USA 1997 94 10931 10.1073/pnas.94.20.10931 9380737 (Pubitemid 27430838)
7. Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes. Deitsch K, Driskill C, Wellems T, Nucl Acids Res 2001 29 850 10.1093/nar/29.3.850 11160909
8. Validation of a modified method for Bxb1 mycobacteriophage integrase-mediated recombination in Plasmodium falciparum by localization of the H-protein of the glycine cleavage complex to the mitochondrion. Spalding MD, Allary M, Gallagher JR, Prigge ST, Mol Biochem Parasitol 2010 172 156 10.1016/j.molbiopara.2010.04.005 20403390
9. An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum. Hasenkamp S, Wong EH, Horrocks P, Malar J 2012 11 42 10.1186/1475-2875-11-42 22325061
10. Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase. Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR, Fidock DA, Nat Methods 2006 3 615 10.1038/nmeth904 16862136 (Pubitemid 44123428)
11. Synchronization of Plasmodium falciparum erythrocytic stages in culture. Lambros C, Vanderberg JP, J Parasitol 1979 65 418 10.2307/3280287 383936 (Pubitemid 10222999)
12. Effect of var gene disruption on switching in Plasmodium falciparum. Horrocks P, Pinches R, Kyes S, Kriek N, Lee S, Christodoulou Z, Newbold CI, Mol Microbiol 2002 45 1131 10.1046/j.1365-2958.2002.03085.x 12180930 (Pubitemid 35007454)
13. Analysis of the molecular mechanisms governing the stage-specific expression of a prototypical housekeeping gene during intraerythrocytic development of P. falciparum. Wong EH, Hasenkamp S, Horrocks P, J Mol Biol 2011 408 205 10.1016/j.jmb.2011.02.043 21354176
14. Plate-based transfection and culturing technique for genetic manipulation of Plasmodium falciparum. Caro F, Miller MG, DeRisi JL, Malar J 2012 11 22 10.1186/1475-2875-11-22 22257490
15. Plasmodium falciparum: The benefits of shaking. Allen RJW, Kirk K, Mol Biochem Parasitol 2010 169 63 10.1016/j.molbiopara.2009.09.005 19766147